Conventional methods for antimicrobial susceptibility testing of Mycobacterium tuberculosis

2000 ◽  
pp. 133-143 ◽  
Author(s):  
Leonid Heifets
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Conventional Methods

scholarly journals Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa

2004 ◽  
Vol 48 (5) ◽  
pp. 1879-1881 ◽  
Author(s):  
Michael M. Tunney ◽  
Gordon Ramage ◽  
Tyler R. Field ◽  
Thomas F. Moriarty ◽  
Douglas G. Storey
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Excellent Agreement ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Colorimetric Assay ◽  
Antimicrobial Susceptibility Testing ◽  
Tetrazolium Salt ◽  
Xtt Assay ◽  
Conventional Methods

ABSTRACT A colorimetric assay based on the reduction of a tetrazolium salt {2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)} for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h using conventional methods. The data suggests that an XTT-based assay could provide a useful method for rapidly determining the susceptibility of P. aeruginosa to bactericidal antibiotics.

scholarly journals On the Consequences of Poorly Defined Breakpoints for Rifampin Susceptibility Testing of Mycobacterium tuberculosis Complex

2021 ◽  
Vol 59 (4) ◽  
Author(s):  
Claudio U. Köser ◽  
Sophia B. Georghiou ◽  
Thomas Schön ◽  
Max Salfinger
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
World Health ◽  
The European Union ◽  
Reference Standard ◽  
Resistance Mutations ◽  
Content Type ◽  
Indicator Tube

ABSTRACT In a recent report of a systematic review of critical concentrations (CCs), the World Health Organization (WHO) lowered the rifampin (RIF) CC for antimicrobial susceptibility testing (AST) of the Mycobacterium tuberculosis complex using Middlebrook 7H10 medium and the Bactec Mycobacterial Growth Indicator Tube (MGIT) 960 system from 1 to 0.5 μg/ml. The previous RIF CC for 7H10 had been in use for over half a century. Because it had served as the de facto reference standard, it contributed to the endorsement of inappropriately high CCs for other AST methods, including the U.S. Food and Drug Administration (FDA)-approved MGIT system. Moreover, this resulted in confusion about the interpretation of seven borderline resistance mutations in rpoB (i.e., L430P, D435Y, H445L, H445N, H445S, L452P, and I491F). In this issue of the Journal of Clinical Microbiology, Shea et al. (J Clin Microbiol 59:e01885-20, 2021, https://doi.org/10.1128/JCM.01885-20) provide evidence that the CC endorsed by the Clinical and Laboratory Standards Institute for the Sensititre MYCOTB system, which is not FDA approved but is CE-IVD marked in the European Union, is likely also too high. These findings underscore the importance of calibrating AST methods against a rigorously defined reference standard, as recently proposed by the European Committee on Antimicrobial Susceptibility Testing, as well as the value of routine next-generation sequencing for investigating discordant AST results.

scholarly journals Evaluation of the Accelerate PhenoTM system for rapid identification and antimicrobial susceptibility testing of positive blood culture bottles inoculated with primary sterile specimens from patients with suspected severe infections

2021 ◽  
Author(s):  
V Chapot ◽  
L Effenberg ◽  
J Dohmen-Ruetten ◽  
J Buer ◽  
J Kehrmann
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Rapid Identification ◽  
Antimicrobial Susceptibility Testing ◽  
Pathogen Identification ◽  
Severe Infections ◽  
Conventional Methods ◽  
Blood Specimens

The Accelerate PhenoTM system (APS, Accelerate Diagnostics) is approved for the rapid identification and phenotypic antimicrobial susceptibility testing (AST) of microorganisms grown from positive blood cultures inoculated with blood from septic patients. We evaluated the performance for identification and AST from positive blood culture bottles inoculated with primary sterile non-blood specimens from patients with suspected severe infections. One hundred positive blood culture bottles with primary sterile specimens (63 cerebrospinal fluids, 16 ascites, 7 pleural fluids, 4 vitreous fluids, 5 joint aspirates and 5 other aspirates) from 100 patients were included. Pathogen identification was in agreement with conventional methods for 72 of 100 cultures (72%) and for 81 of 112 (72%) pathogens when considering all pathogens, for 72 of 92 (78%) cultures and 81 of 104 (78%) pathogens when considering on-panel pathogens only. Eight of 31 isolates (26%) not identified by APS were pathogens not included in the APS panel. APS and conventional methods accordingly identified all pathogens from two of nine polymicrobial cultures (22%). APS generated antimicrobial resistance results for 57 pathogens of 57 cultures. The overall category agreement between APS and culture based AST was 91.2%, the rates for minor errors 6.9%, major 1.7% and very major errors 0.2%. APS may accelerate pathogen identification and phenotypic AST from positive blood culture bottles inoculated with primary sterile specimens from patients with serious infections, especially for hospitals without on-site microbiology laboratory. However, the inclusion of non-blood specimens with a high likelihood of polymicrobial infections may result in an inferior performance.

scholarly journals Challenging a dogma: antimicrobial susceptibility testing breakpoints for Mycobacterium tuberculosis

2012 ◽  
Vol 90 (9) ◽  
pp. 693-698 ◽  
Author(s):  
Kristian Ängeby ◽  
Pontus Juréen ◽  
Gunnar Kahlmeter ◽  
Sven Hoffner ◽  
Thomas Schön
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing

scholarly journals Verification of Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

2006 ◽  
Vol 44 (5) ◽  
pp. 1921-1921
Author(s):  
L. Hall ◽  
K. A. Doerr ◽  
W. S. Harmsen ◽  
N. L. Wengenack ◽  
G. D. Roberts
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing
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