scholarly journals Antimicrobial susceptibility testing of Mycobacterium tuberculosis complex isolates – the EUCAST broth microdilution reference method for MIC determination

2020 ◽  
Vol 26 (11) ◽  
pp. 1488-1492 ◽  
Author(s):  
Thomas Schön ◽  
Jim Werngren ◽  
Diana Machado ◽  
Emanuele Borroni ◽  
Maria Wijkander ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Mycobacterium Tuberculosis Complex ◽  
Reference Method ◽  
Antimicrobial Susceptibility Testing ◽  
Broth Microdilution ◽  
Tuberculosis Complex

scholarly journals Reliability of the MB/BacT System for Testing Susceptibility of Mycobacterium tuberculosis Complex Isolates to Antituberculous Drugs

2000 ◽  
Vol 38 (2) ◽  
pp. 872-873 ◽  
Author(s):  
Francesca Brunello ◽  
Roberta Fontana
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Mycobacterium Tuberculosis Complex ◽  
Antimicrobial Susceptibility Testing ◽  
Clinical Isolates ◽  
Tuberculosis Complex ◽  
Antituberculous Drugs ◽  
Testing Susceptibility

The susceptibility of 115 Mycobacterium tuberculosiscomplex clinical isolates to isoniazid, streptomycin, ethambutol, and rifampin was assessed by the MB/BacT and BACTEC 460TB systems. The correlation between the two tests was 98.3% for isoniazid, 100% for streptomycin and rifampin, and 95.8% for ethambutol. Turnaround times for antimicrobial susceptibility testing ranged from 5 to 11 days (median, 8.5 days) for MB/BacT and from 4 to 8 days (median, 6 days) for BACTEC 460TB.

scholarly journals Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 975
Author(s):  
Florian Baquer ◽  
Asma Ali Sawan ◽  
Michel Auzou ◽  
Antoine Grillon ◽  
Benoît Jaulhac ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Reference Method ◽  
Antimicrobial Susceptibility Testing ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Broth Microdilution ◽  
Multicenter Studies ◽  
Gradient Diffusion ◽  
Agar Dilution

Antimicrobial susceptibility testing of anaerobes is challenging. Because MIC determination is recommended by both CLSI and EUCAST, commercial broth microdilution and diffusion strip tests have been developed. The reliability of broth microdilution methods has not been assessed yet using the agar dilution reference method. In this work, we evaluated two broth microdilution kits (MICRONAUT-S Anaerobes® MIC and Sensititre Anaerobe MIC®) and one gradient diffusion strip method (Liofilchem®) for antimicrobial susceptibility testing of 47 Clostridiales isolates (Clostridium, Clostridioides and Hungatella species) using the agar dilution method as a reference. The evaluation focused on comparing six antimicrobial molecules available in both microdilution kits. Analytical performances were evaluated according to the Food and Drug Administration (FDA) recommendations. Essential agreements (EA) and categorical agreements (CA) varied greatly according to the molecule and the evaluated method. Vancomycin had values of essential and categorical agreements above 90% for the three methods. The CA fulfilled the FDA criteria for three major molecules in the treatment of Gram-positive anaerobic infections (metronidazole, piperacillin/tazobactam and vancomycin). The highest rate of error was observed for clindamycin. Multicenter studies are needed to further validate these results.

scholarly journals What is the role of the EUCAST reference method for MIC testing of the Mycobacterium tuberculosis complex?

2020 ◽  
Vol 26 (11) ◽  
pp. 1453-1455 ◽  
Author(s):  
Thomas Schön ◽  
Claudio Umberto Köser ◽  
Jim Werngren ◽  
Miguel Viveiros ◽  
Sophia Georghiou ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Reference Method ◽  
Tuberculosis Complex
Sign in / Sign up

Export Citation Format

Share Document