Transcriptional transgene silencing and chromatin components

2000 ◽  
pp. 101-114
Author(s):  
Peter Meyer
Keyword(s):  
Transgene Silencing

scholarly journals Post-transcriptional transgene silencing and consequent tospovirus resistance in transgenic lettuce are affected by transgene dosage and plant development

1996 ◽  
Vol 9 (6) ◽  
pp. 899-909 ◽  
Author(s):  
Sheng-Zhi Pang ◽  
Fuh-Jyh Ja ◽  
Kim Carney ◽  
John Stout ◽  
David M. Tricoli ◽  
...  
Keyword(s):  
Plant Development ◽  
Transgene Silencing ◽  
Transgenic Lettuce

A novel T-DNA vector design for selection of transgenic lines with simple transgene integration and stable transgene expression

2005 ◽  
Vol 32 (8) ◽  
pp. 671 ◽  
Author(s):  
Song Chen ◽  
Christopher A. Helliwell ◽  
Li-Min Wu ◽  
Elizabeth S. Dennis ◽  
Narayana M. Upadhyaya ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Marker Gene ◽  
Direct Repeat ◽  
Transgene Silencing ◽  
Selective Marker ◽  
Hairpin Rna ◽  
Transgenic Lines ◽  
Flanking Sequences ◽  
Dna Vector ◽  
Selection Of

Plants transformed with Agrobacterium frequently contain T-DNA concatamers with direct-repeat (d / r) or inverted-repeat (i / r) transgene integrations, and these repetitive T-DNA insertions are often associated with transgene silencing. To facilitate the selection of transgenic lines with simple T-DNA insertions, we constructed a binary vector (pSIV) based on the principle of hairpin RNA (hpRNA)-induced gene silencing. The vector is designed so that any transformed cells that contain more than one insertion per locus should generate hpRNA against the selective marker gene, leading to its silencing. These cells should, therefore, be sensitive to the selective agent and less likely to regenerate. Results from Arabidopsis and tobacco transformation showed that pSIV gave considerably fewer transgenic lines with repetitive insertions than did a conventional T-DNA vector (pCON). Furthermore, the transgene was more stably expressed in the pSIV plants than in the pCON plants. Rescue of plant DNA flanking sequences from pSIV plants was significantly more frequent than from pCON plants, suggesting that pSIV is potentially useful for T-DNA tagging. Our results revealed a perfect correlation between the presence of tail-to-tail inverted repeats and transgene silencing, supporting the view that read-through hpRNA transcript derived from i / r T-DNA insertions is a primary inducer of transgene silencing in plants.

Different Types of Transgene Silencing in Animals: A Natural Foundation for RNAi Technology

2015 ◽  
Vol 04 (04) ◽  
Author(s):  
Sampada Sontakke ◽  
Kishan K Khetan ◽  
Subhodeep Banerjee ◽  
Tanmoy Mondal
Keyword(s):  
Transgene Silencing ◽  
Rnai Technology ◽  
Different Types ◽  
Natural Foundation

Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance

2004 ◽  
Vol 13 (3) ◽  
pp. 229-242 ◽  
Author(s):  
Li-Jen Liao ◽  
I-Chun Pan ◽  
Yuan-Li Chan ◽  
Yau-Heiu Hsu ◽  
Wen-Huei Chen ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Transgene Silencing ◽  
Cymbidium Mosaic Virus

scholarly journals Transgene silencing: New insights into an old puzzle

1998 ◽  
Vol 23 (5) ◽  
pp. 539-540 ◽  
Author(s):  
Tirumalai R. Raghunand
Keyword(s):  
Transgene Silencing

scholarly journals RNAi Related Mechanisms Affect Both Transcriptional and Posttranscriptional Transgene Silencing in Drosophila

2002 ◽  
Vol 9 (2) ◽  
pp. 315-327 ◽  
Author(s):  
Manika Pal-Bhadra ◽  
Utpal Bhadra ◽  
James A Birchler
Keyword(s):  
Transgene Silencing
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