Sulphate Assimilation: A Pathway Which Likes to Surprise

2003 ◽  
pp. 87-112 ◽  
Author(s):  
S. Kopriva ◽  
A. Koprivova
Keyword(s):  
Sulphate Assimilation

scholarly journals The basis for an apparent auxotrophy for reduced sulphur metabolites insF−mutants ofAspergillus nidulans

1977 ◽  
Vol 30 (2) ◽  
pp. 207-210 ◽  
Author(s):  
Herbert N. Arst
Keyword(s):  
Aspergillus Nidulans ◽  
Toxic Metabolite ◽  
Cysteine Biosynthesis ◽  
Ofaspergillus Nidulans ◽  
Sulphate Assimilation

SUMMARYsF−mutants ofAspergillus nidulansare subject to toxicity by a metabolite derived from sulphate but are not blocked in cysteine biosynthesis. Bearing in mind the unavoidable presence of sulphate in agar-solidified media, their apparent auxotrophy for thiosulphate, L-cysteine or L-methionine stems from the ability of these reduced sulphur-containing metabolites to repress, directly or indirectly, the syntheses of sulphate permease and the enzymes of sulphate assimilation, thereby preventing synthesis of the toxic metabolite.

scholarly journals A new gene controlling sulphite reductase inAspergillus nidulans

1988 ◽  
Vol 51 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Janina Nadolska-Lutyk ◽  
Andrzej Paszewski
Keyword(s):  
Aspergillus Nidulans ◽  
Alternative Pathway ◽  
Full Activity ◽  
Sulphate Assimilation ◽  
Cysteine Synthesis ◽  
New Gene ◽  
Sulphite Reductase

SummaryA new gene designatedsGwas identified inAspergillus nidulansby mutation affecting the enzyme sulphite reductase and leading to a strong derepression of arylsulphatase and enzymes constituting the alternative pathway of cysteine synthesis. The results indicate that proper physiological functioning of this pathway is strongly dependent on full activity of the sulphate assimilation pathway.

Regulation of sulphate assimilation inSaccharomyces cerevisiae

Yeast ◽  
1996 ◽  
Vol 12 (11) ◽  
pp. 1153-1162 ◽  
Author(s):  
Bun-Ichiro Ono ◽  
Kazuyasu Kijima ◽  
Nobuya Ishii ◽  
Takahiro Kawato ◽  
Akio Matsuda ◽  
...  
Keyword(s):  
Sulphate Assimilation

Effect of humic substances on photosynthetic sulphate assimilation in maize seedlings

1991 ◽  
Vol 71 (2) ◽  
pp. 239-242 ◽  
Author(s):  
M. Ferretti ◽  
C. Passera ◽  
R. Ghisi ◽  
S. Nardi
Keyword(s):  
Organic Carbon ◽  
Protein Content ◽  
Key Words ◽  
Humic Substances ◽  
Protein Level ◽  
Maize Seedlings ◽  
Sulphate Assimilation ◽  
Maize Plants ◽  
Key Words Atp ◽  
Atp Sulphurylase

Maize plants were grown in nutrient solution containing 0, 15, 30, 50 mg organic carbon L−1 of humic substances (HS) (M.W. < 12 KDa) extracted from a cambisol. After 14 d of growth the second and third leaves were collected and analyzed for chlorophyll and protein content and for the activities of ATP sulphurylase (ATP-s) and O-acetylserine sulphydrylase (OAS-s) to evaluate the effect of HS on maize sulphate assimilation pathway. Humic substances induced significant decreases in chlorophyll content, whereas the protein level slightly increased up to 30 mg L−1 of HS. ATP-s and OAS-s were positively affected by HS. The stimulations were different according to the enzyme and leaf investigated. Our results suggest that HS might play an important role in the efficiency of maize plants to assimilate sulphate. Key words: ATP-sulphurylase, chlorophyll, humic substances, maize, O-acetylserine sulphydrilase

scholarly journals The Effect of Introgression of an R2R3 MYB Transcription Factor on Sulphur Metabolism in Brassica oleracea

2020 ◽  
Author(s):  
Mikhaela Neequaye ◽  
Shikha Saha ◽  
Martin Trick ◽  
Burkhard Steuernagel ◽  
Perla Troncoso-Rey ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Oleracea ◽  
Genetic Variant ◽  
Myb Transcription Factor ◽  
Sulphur Metabolism ◽  
Expression Of Genes ◽  
Downstream Effects ◽  
Sulphate Assimilation ◽  
Enhanced Expression ◽  
R2r3 Myb

AbstractBackgroundA diet rich in cruciferous vegetables is reported to have beneficial health effects, partially mediated by 4-methylsulfinylbutyl glucosinolate, or glucoraphanin, which is predominantly found within broccoli (Brassica oleracea var italica). We describe the downstream effects on transcription and metabolism in broccoli following the introgression of a genetic variant of MYB28 into broccoli from a wild Brassica relative which has previously been associated with enhancement of glucoraphanin.ResultsWhole genome sequencing, RNA expression and metabolite analyses were used to characterise the consequences of the introgression of either one or two copies of a genetic variant of the MYB28 transcription factor into a commercial broccoli genetic background. The introgression of the variant of MYB28 resulted in enhanced expression of genes involved in primary sulphate assimilation, sulphur metabolism and aliphatic glucosinolate biosynthesis, and enhanced accumulation of 4-methylsulphinyl butyl glucosinolate in florets. Other changes in transcription that may be related to non-targeted introgression events are reported. There were no consistent effects upon sulphur metabolites pools, apart from methionine-derived glucosinolates.ConclusionThis study illustrates the downstream effects on transcription and metabolism of the introgression of a genetic variant of MYB28 from a wild species into a commercial broccoli genotype.

Genetics of sulphate assimilation inSchizosaccharomyces Pombe

2002 ◽  
Vol 49 (2-3) ◽  
pp. 279-283 ◽  
Author(s):  
T. Simonics ◽  
Luca Bánszky ◽  
Anna Maráz
Keyword(s):  
Sulphate Assimilation

scholarly journals Effects of adenine nucleotides and phosphate on adenosine triphosphate sulphurylase from Anabaena cylindrica

1977 ◽  
Vol 164 (1) ◽  
pp. 161-167 ◽  
Author(s):  
S K Sawhney ◽  
D J D Nicholas
Keyword(s):  
Enzyme Activity ◽  
Energy Metabolism ◽  
Adenosine Triphosphate ◽  
Adenine Nucleotides ◽  
Anabaena Cylindrica ◽  
Sulphate Assimilation ◽  
Atp Sulphurylase

Production of adenosine 5'-[35S]sulphatophosphate by a partially purified ATP sulphurylase from Anabaena cylindrica was inhibited by AMP, ADP and P1. Decreases in enzyme activity in the presence of these inhibitors were reversed by increasing the concentrations of ATP. The adenine nucleotides inhibited the enzyme competitively with respect to ATP. In the presence of P1, ATP showed a positive co-operative effect on enzyme activity. The inhibition by P1 was enhanced by increasing concentrations of MG2+. The effects of the adenine nucleotides and the interaction of P1 and Mg2+ on ATP sulphurylase activity are discussed in relation to the regulation of sulphate assimilation via the energy metabolism of the alga.

Role of the sulphate assimilation pathway in utilization of glutathione as a sulphur source bySaccharomyces cerevisiae

Yeast ◽  
1999 ◽  
Vol 15 (14) ◽  
pp. 1449-1457 ◽  
Author(s):  
Tsuyoshi Miyake ◽  
Hiroyuki Sammoto ◽  
Munetoshi Kanayama ◽  
Ken-ichi Tomochika ◽  
Sumio Shinoda ◽  
...  
Keyword(s):  
Sulphur Source ◽  
Sulphate Assimilation

scholarly journals Flux control of sulphate assimilation inArabidopsis thaliana: adenosine 5′-phosphosulphate reductase is more susceptible than ATP sulphurylase to negative control by thiols

2002 ◽  
Vol 31 (6) ◽  
pp. 729-740 ◽  
Author(s):  
Pierre Vauclare ◽  
Stanislav Kopriva ◽  
David Fell ◽  
Marianne Suter ◽  
Liliane Sticher ◽  
...  
Keyword(s):  
Negative Control ◽  
Flux Control ◽  
Sulphate Assimilation ◽  
Atp Sulphurylase
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