Role of Lignin Peroxidase and Manganese Peroxidase of Phanerochaete Chrysosporium in the Decolorization of Olive Mill Wastewaters

Reduction of H2O2-oxidized manganese peroxidase (MnP), lignin peroxidase and, to some extent, horseradish peroxidase, was studied in the presence of cellobiose oxidase (CbO) and cellobiose. It was found that the reversion rates for MnP compound II and lignin peroxidase compound II back to native enzymes increased significantly in the presence of CbO and cellobiose. However, the reduction of cytochrome c by CbO plus cellobiose was 40 times faster than the reduction of MnP compound II. Also, the lag phase before reversion to the native states decreased for all three peroxidases in the presence of CbO and cellobiose. Active CbO did not repress formation of compounds I or II of the peroxidases, and Mn2+/veratryl alcohol reduced compound II of the peroxidases much more rapidly than did active CbO. This indicates that, in the presence of Mn2+ or veratryl alcohol, MnP and lignin peroxidase can complete their catalytic cycles and function normally without interference from CbO. Without the presence of peroxidase substrates, active CbO reduced compound II of the above peroxidases.

Download Full-text

Evidence for a role of manganese peroxidase in the decolorization of Kraft pulp bleach plant effluent by Phanerochaete chrysosporium: Effects of initial culture conditions on enzyme production

Journal of Biotechnology ◽

10.1016/0168-1656(94)90130-9 ◽

1994 ◽

Vol 37 (3) ◽

pp. 229-234 ◽

Cited By ~ 32

Author(s):

Charles J. Jaspers ◽

Gloria Jimenez ◽

Michel J. Penninckx

Keyword(s):

Phanerochaete Chrysosporium ◽

Manganese Peroxidase ◽

Enzyme Production ◽

Kraft Pulp ◽

Culture Conditions ◽

Initial Culture

Download Full-text

Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus

Canadian Journal of Microbiology ◽

10.1139/w09-094 ◽

2009 ◽

Vol 55 (12) ◽

pp. 1397-1402 ◽

Cited By ~ 2

Author(s):

Paramjit Kaur Bajwa ◽

Daljit Singh Arora

Keyword(s):

Phanerochaete Chrysosporium ◽

Lignin Peroxidase ◽

Manganese Peroxidase ◽

Ligninolytic Enzymes ◽

Culture Conditions ◽

Malt Extract ◽

Mineral Salts ◽

Wide Range ◽

Extract Medium ◽

Malt Extract Medium

The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium . Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.

Download Full-text