Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus

2009 ◽  
Vol 55 (12) ◽  
pp. 1397-1402 ◽  
Author(s):  
Paramjit Kaur Bajwa ◽  
Daljit Singh Arora
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Ligninolytic Enzymes ◽  
Culture Conditions ◽  
Malt Extract ◽  
Mineral Salts ◽  
Wide Range ◽  
Extract Medium ◽  
Malt Extract Medium

The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium . Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.

A defined growth medium for the production of chloroperoxidase by Caldariomyces fumago

1981 ◽  
Vol 27 (12) ◽  
pp. 1298-1305 ◽  
Author(s):  
Michael A. Pickard
Keyword(s):  
Enzyme Activity ◽  
Carbon Source ◽  
Growth Medium ◽  
Enzyme Production ◽  
Defined Medium ◽  
Malt Extract ◽  
Caldariomyces Fumago ◽  
High Enzyme ◽  
Extract Medium ◽  
Malt Extract Medium

Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose – malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement of glucose by fructose as the carbon source provided a defined medium which, by comparison with the complex medium, produced the following results with both fungal strains. Chloroperoxidase was produced to similar levels, with maximum production after 6 days rather than 12 days of growth; pigmentation of the medium was reduced by 90% and the pH of the medium remained constant, thus stabilizing enzyme activity. Addition of urea or proline as a nitrogen supplement to nitrate enhanced enzyme production by strain CMI 89362. Comparison of the two strains indicated that CMI 89362 produced higher levels of chloroperoxidase than ATCC 16373.

scholarly journals Lignolytic Enzymes Production from Selected Mushrooms

2015 ◽  
Vol 3 (2) ◽  
pp. 308-313 ◽  
Author(s):  
H.M. Shantaveera Swamy ◽  
Ramalingappa
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Western Ghats ◽  
Agar Plate ◽  
Ligninolytic Enzymes ◽  
Enzyme Assays ◽  
Lignolytic Enzymes ◽  
Plate Assay

In this paper, ligninase enzymes produced by selected mushrooms have been reported. We collected mushrooms from Western Ghats, most of them were edible food. Thirty samples isolated were tested using a plate assay through direct agar plate assay by using ABTS, decolourisation containing the fifteen isolates were able to decolourise the dye, indicating a lignin-degrading ability. Spectrophotometric enzyme assays from all selected isolates were carried out to examine the production of Ligninolytic enzymes (Laccase, lignin peroxidase and manganese peroxidase). Ten selected isolates produced all three kinds of enzymes tested. Lignolytic enzymes are groups of enzymes these are actively involved in bioremediation.Int J Appl Sci Biotechnol, Vol 3(2): 308-313 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12732 

SOME FACTORS AFFECTING THE GERMINATION OF SPORES OF FOMES IGNIARIUS VAR. POPULINUS (NEUMAN) CAMPBELL, AND THE SIGNIFICANCE OF THESE FACTORS IN INFECTION

1958 ◽  
Vol 36 (3) ◽  
pp. 421-437 ◽  
Author(s):  
H. M. Good ◽  
W. Spanis
Keyword(s):  
Amino Acids ◽  
Sugar Content ◽  
Alkaline Media ◽  
Malt Extract ◽  
Factors Affecting ◽  
Extract Medium ◽  
Malt Extract Medium

Spores of F. igniarius collected from different conks at one time, or from one conk at different times of the year, varied significantly in germinability. However, spores collected from one conk within a 3-day period were judged to be sufficiently consistent for use in comparative tests.Spores germinated best on an 8% malt extract medium buffered at pH 4.0. Only a trace of germination occurred on water agar and none occurred on alkaline media. Stimulation by malt extract appeared to be due partly to the sugar content and partly to an unidentified factor.While extracts of the surface wood of aged wounds contained far less of sugars and amino acids than did extracts of fresh sapwood, germination on the former was consistently better. A lower pH appeared to account for most of this advantage but even when the two types of extract were brought to the same pH better germination occurred on the extract of the aged wound.Spores of F. igniarius germinated well at temperatures in the range 20–35 °C. They varied widely in their ability to germinate after storage. Some samples germinated fairly well after 80 days; others failed to germinate after 10 days.These findings are discussed in relation to the problems of infection.

scholarly journals Quantitative Evaluation of the Production of Ligninolytic Enzymes- Lignin Peroxidase and Manganese Peroxidase by P. Sajor Caju During Coir Pith Composting

CORD ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 10 ◽  
Author(s):  
Radhakrishnan S
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Tamil Nadu ◽  
Lignin Content ◽  
Ligninolytic Enzymes ◽  
Oxidative Enzymes ◽  
Aquatic Life ◽  
Coir Pith ◽  
Coir Fibre ◽  
Pleurotus Sajor Caju

Coir is the natural hard fruit fibre extracted from the exocarp of the coconut. The fibre has over 40 percent lignin and is spun into yarn and rope. Coir is used globally for manufacturing floor coverings as home furnishing. The Coir Industry enjoys the status as the largest cottage industry in Kerala giving employment to over a million people, of which 80 percent constitute women. Coir pith is a biomass residue generated during the extraction of coir fibre from coconut husk. Coir pith produced during coir fibre extraction is of environmental concern as its dumping on shore line and leaching of its constituents alter water quality and aquatic life. Management of coir pith is a major problem with all coir industrialists. Hillocks of coir pith accumulate in the vicinities of coir fibre extraction units in Kerala, Tamil Nadu, Andhra Pradesh, Karnataka, and Orissa. These agricultural wastes have traditionally been disposed by burning which resulted in various environmental problems. Therefore, composting is an alternate way to dispose coir pith and is of critical importance. Ligninolytic enzyme production during coir pith composting by Pleurotus sajor caju has been studied in detail. Pleurotus sajor caju produces oxidative enzymes which degrade lignin in the presence of urea as nitrogen source. Substitution of urea with vegetative sources has resulted in the vigorous growth of the mushroom which leads to decreased lignin content and C: N ratio in the biodegraded coir pith. Combination of Azolla and Soya hulls as biological supplements was observed to be the best substitute for lignin peroxidase and manganese peroxidase production. Activity of manganese peroxidase and lignin peroxidase was maximum on the twentieth day of fermentation of coir pith. The level of enzyme activity during biological composting using vegetative sources was compared with the conventional process using urea. The enzyme profile exhibited variation with change in substrate and duration of decomposition. The colonization of Pleurotus sajor caju by its utilization leads to biochemical changes in coir pith converting it into an ideal plant nutrient.

scholarly journals The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME

2021 ◽  
Vol 13 (2) ◽  
pp. 243-249
Author(s):  
Yohanes Bernard Subowo ◽  
Arwan Sugiharto
Keyword(s):  
Palm Oil ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Laccase Activity ◽  
Ligninolytic Enzymes ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Growth Media ◽  
Palm Oil Mill ◽  
Low Activity

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME. 

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