Living Cells in Opto-Electrical Cages Characterisation Manipulation and Force Measurements

2000 ◽  
pp. 261-264 ◽  
Author(s):  
Günter R. Fuhr ◽  
Christoph Reichte
Keyword(s):  
Living Cells ◽  
Force Measurements

scholarly journals Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers

2020 ◽  
Vol 8 ◽  
Author(s):  
Carolin Riesenberg ◽  
Christian Alejandro Iriarte-Valdez ◽  
Annegret Becker ◽  
Maria Dienerowitz ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Receptor Interaction ◽  
Force Measurements

scholarly journals High-speed force spectroscopy: microsecond force measurements using ultrashort cantilevers

2019 ◽  
Vol 11 (5) ◽  
pp. 689-699 ◽  
Author(s):  
Claire Valotteau ◽  
Fidan Sumbul ◽  
Felix Rico
Keyword(s):  
Time Scales ◽  
High Speed ◽  
Protein Unfolding ◽  
Deep Understanding ◽  
Force Spectroscopy ◽  
Md Simulations ◽  
Living Cells ◽  
Force Measurements ◽  
Cellular Mechanics ◽  
Wide Range

Abstract Complete understanding of the role of mechanical forces in biological processes requires knowledge of the mechanical properties of individual proteins and living cells. Moreover, the dynamic response of biological systems at the nano- and microscales span over several orders of magnitude in time, from sub-microseconds to several minutes. Thus, access to force measurements over a wide range of length and time scales is required. High-speed atomic force microscopy (HS-AFM) using ultrashort cantilevers has emerged as a tool to study the dynamics of biomolecules and cells at video rates. The adaptation of HS-AFM to perform high-speed force spectroscopy (HS-FS) allows probing protein unfolding and receptor/ligand unbinding up to the velocity of molecular dynamics (MD) simulations with sub-microsecond time resolution. Moreover, application of HS-FS on living cells allows probing the viscoelastic response at short time scales providing deep understanding of cytoskeleton dynamics. In this mini-review, we assess the principles and recent developments and applications of HS-FS using ultrashort cantilevers to probe molecular and cellular mechanics.

scholarly journals Single Molecule Force Measurements in Living Cells Reveal a Minimally Tensioned Integrin State

ACS Nano ◽  
2016 ◽  
Vol 10 (12) ◽  
pp. 10745-10752 ◽  
Author(s):  
Alice C. Chang ◽  
Armen H. Mekhdjian ◽  
Masatoshi Morimatsu ◽  
Aleksandra Kirillovna Denisin ◽  
Beth L. Pruitt ◽  
...  
Keyword(s):  
Single Molecule ◽  
Living Cells ◽  
Force Measurements

Direct Measurement of Mechanical and Adhesive Properties of Living Cells Using Surface Forces Apparatus

2007 ◽  
Vol 60 (9) ◽  
pp. 638 ◽  
Author(s):  
Xavier Banquy ◽  
Jean-Michel Rabanel ◽  
Patrice Hildgen ◽  
Suzanne Giasson
Keyword(s):  
Single Cells ◽  
Living Cells ◽  
Surface Forces ◽  
Force Measurements ◽  
Surface Forces Apparatus ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Substrate ◽  
Mediate Cell

The adhesive and mechanical properties of living cells assembled into a monolayer on two different substrates were investigated using the surface forces apparatus (SFA) technique. The force measurements allowed elastic and bending moduli of the cells plated on substrates to be determined. The moduli are in good agreement with data reported in the literature for single cells determined using atomic force microscopy. Results confirm that the nature of the cell–substrate interactions can mediate cell mechanical and adhesive properties.

scholarly journals Force measurements with optical tweezers inside living cells

2014 ◽  
Author(s):  
Josep Mas ◽  
Arnau Farré ◽  
Jordi Sancho-Parramon ◽  
Estela Martín-Badosa ◽  
Mario Montes-Usategui
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Force Measurements

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

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