Challenges in DNA Extraction from Forensic Samples

2021 ◽  
pp. 1-20
Author(s):  
Anna Barbaro
Keyword(s):  
Dna Extraction ◽  
Forensic Samples

scholarly journals DNA Extraction From Various Forensic Samples Using Automated Extraction Instruments.

2012 ◽  
Vol 17 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Koji Fujii ◽  
Shota Inokuchi ◽  
Tetsushi Kitayama ◽  
Hiroaki Nakahara ◽  
Natsuko Mizuno ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Automated Extraction ◽  
Forensic Samples

DNA Extraction from Forensic Samples Using Chelex

2009 ◽  
Vol 2009 (6) ◽  
pp. pdb.prot5229-pdb.prot5229 ◽  
Author(s):  
J. M. Butler
Keyword(s):  
Dna Extraction ◽  
Forensic Samples

scholarly journals DNA extraction from human saliva deposited on skin and its use in forensic identification procedures

2005 ◽  
Vol 19 (3) ◽  
pp. 216-222 ◽  
Author(s):  
Evelyn Anzai-Kanto ◽  
Mário Hiroyuki Hirata ◽  
Rosario Dominguez Crespo Hirata ◽  
Fabio Daumas Nunes ◽  
Rodolfo Francisco Haltenhoff Melani ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Dna Typing ◽  
Human Saliva ◽  
Forensic Identification ◽  
Practical Case ◽  
Likelihood Ratios ◽  
Criminal Cases ◽  
Dna Recovery ◽  
Forensic Samples ◽  
Dna Collection

Saliva is usually deposited in bite marks found in many homicides, assault and other criminal cases. In the present study, saliva obtained from volunteers was deposited on skin and recovered for DNA extraction and typing in order to evaluate its usefulness for practical case investigation and discuss the contribution of forensic dentistry to saliva DNA typing. Twenty saliva samples were colleted from different donors and used as suspects' samples. Five of these samples were randomly selected and deposited (250 µl) on arm skin. Saliva was collected from skin using the double swab technique. DNA from saliva and skin-deposited saliva samples was extracted by the phenol-chloroform method. DNA samples were amplified by PCR for DNA typing using a set of 15 STRs. The recovery of DNA from saliva deposited in the skin was 14 to 10 times lower than DNA quantity from saliva samples. DNA typing was demonstrated in 4 of 5 deposited saliva samples, the likelihood ratios estimated for these samples based on data of the Brazilian population were 1:11, 1:500, 1:159.140 and 1:153.700.123. Our results indicate that standardized procedures used for DNA collection and extraction from skin-deposited saliva can be used as a method to recover salivary DNA in criminal cases. However, it is important to observe that DNA recovery in forensic samples can be difficult. This study suggests that the analysis of saliva deposited on skin be incorporated into a criminal investigation since it may have great discriminatory power.

DNA Extraction from Blood and Forensic Samples

2011 ◽  
pp. 45-54 ◽  
Author(s):  
Solange Sorçaburu Cigliero ◽  
Elisabetta Edalucci ◽  
Paolo Fattorini
Keyword(s):  
Dna Extraction ◽  
Forensic Samples

scholarly journals Comparison between automated DNA extraction employing the EZ1 platform and manual methods using real forensic samples

2021 ◽  
Vol 10 (1) ◽  
pp. 44-56
Author(s):  
PABLO ABDON DA COSTA FRANCEZ ◽  
CIRO AUGUSTO FERNANDES DE OLIVEIRA PENIDO ◽  
GILCELENE DO SOCORRO MEDEIROS DE BRITO COSTA ◽  
RODRIGO MATOS DE ALMEIDA ◽  
EDUARDA EMANUELLE SILVA PENA ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Forensic Samples ◽  
Manual Methods ◽  
Automated Dna Extraction

Relationship of HLA-DRB1 Alleles and Rheumatoid Arthritis in West South of Iran

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Karim Mowla ◽  
Elham Rajaee M. D. ◽  
Mehrdad Dargahi-MalAmir M. D. ◽  
Neda Yousefinezhad ◽  
Maryam Jamali Hondori
Keyword(s):  
Rheumatoid Arthritis ◽  
Environmental Factors ◽  
Rheumatoid Factor ◽  
Dna Extraction ◽  
Wrist Joint ◽  
Blood Sampling ◽  
Healthy Controls ◽  
Relationship Of ◽  
Synovial Pannus

Background: Rheumatoid arthritis is a systemic multifactor disease that presented with symmetrical polyarthritis more preferably in small wrist joint and ankle. Synovial pannus cause destruction and deformities in joints. The main reason of this disease in unknown, but past researchesshowed that genetically factor play important role beside environmental factors in susceptibility to this entity. Method:100 patients with rheumatoid arthritis diagnosed upon ACR 2010 criteria enrolled study. 92 healthy patents also enrolled DNA studying. of both group was extracted through DNA extraction kits by blood sampling. HLA-DRB1 typing was done by PCR-SSP method. Results: There were no significant differences in HLADRB1 *04, HLADRB1*08 and HLADRB1*11 alleles presentation between patients and healthy controls. Only there were statically significant correlation between HLA-DRB1*08 and Rheumatoid factor positive patents. (P = 0.025).

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