Relationship of HLA-DRB1 Alleles and Rheumatoid Arthritis in West South of Iran

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Karim Mowla ◽  
Elham Rajaee M. D. ◽  
Mehrdad Dargahi-MalAmir M. D. ◽  
Neda Yousefinezhad ◽  
Maryam Jamali Hondori
Keyword(s):  
Rheumatoid Arthritis ◽  
Environmental Factors ◽  
Rheumatoid Factor ◽  
Dna Extraction ◽  
Wrist Joint ◽  
Blood Sampling ◽  
Healthy Controls ◽  
Relationship Of ◽  
Synovial Pannus

Background: Rheumatoid arthritis is a systemic multifactor disease that presented with symmetrical polyarthritis more preferably in small wrist joint and ankle. Synovial pannus cause destruction and deformities in joints. The main reason of this disease in unknown, but past researchesshowed that genetically factor play important role beside environmental factors in susceptibility to this entity. Method:100 patients with rheumatoid arthritis diagnosed upon ACR 2010 criteria enrolled study. 92 healthy patents also enrolled DNA studying. of both group was extracted through DNA extraction kits by blood sampling. HLA-DRB1 typing was done by PCR-SSP method. Results: There were no significant differences in HLADRB1 *04, HLADRB1*08 and HLADRB1*11 alleles presentation between patients and healthy controls. Only there were statically significant correlation between HLA-DRB1*08 and Rheumatoid factor positive patents. (P = 0.025).

scholarly journals Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

2015 ◽  
Vol 84 (1) ◽  
pp. 34-40
Author(s):  
Anna Olewicz-Gawlik ◽  
Izabela Korczowska-Łącka ◽  
Paweł Hrycaj
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Acute Phase Proteins ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Male And Female ◽  
Leukocyte Extravasation

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

scholarly journals Cytotoxic T lymphocyte-Associated Antigen +49G Variant Confers Risk for Anti-CCP- and Rheumatoid Factor-Positive Type of Rheumatoid Arthritis Only in Combination with CT60∗G Allele

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Bernadett Farago ◽  
Peter Kisfali ◽  
Lili Magyari ◽  
Noemi Polgar ◽  
Bela Melegh
Keyword(s):  
Rheumatoid Arthritis ◽  
Regression Analysis ◽  
Rheumatoid Factor ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Healthy Controls ◽  
Predisposing Factor ◽  
Positive Type ◽  
Allelic Combination

Controversial observations have been published on the association of the cytotoxic T lymphocyte associated antigen gene's variants with rheumatoid arthritis (RA). After genotyping 428 patients and 230 matched controls, the prevalence of theCT60∗G allele was more frequent in RF- and/or anti-CCP-seropositive RApatients, compared to the healthy controls (P<.001). Regression analysis revealed that theCT60∗G allele is a possible predisposing factor for RA in these subgroups. No accumulation of the+49∗G allele was found among patients, and this variant was not found to correlate with RA. Assaying the possible genotype variations, the+49∗G-CT60∗G allelic combination was accumulated in seropositive RA-subtypes, and was associated with the risk of RA (OR=1.73,P=.001for the whole RA-population). Although the+49∗G allele did not mean a predisposition to RA alone, in combination withCT60∗G it, also conferred risk, suggesting that the+49A/Gvariant is associated with the risk of RA only in certain haplotypes.

Relationship of Rheumatoid Factor to Lung Diffusion Capacity in Smoking and Nonsmoking Patients with Rheumatoid Arthritis

1995 ◽  
Vol 24 (2) ◽  
pp. 119-120 ◽  
Author(s):  
R. Luukkainen ◽  
M. Saltyshev ◽  
R. Pakkasela ◽  
E. Nordqvist ◽  
H. Huhtala ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Lung Diffusion ◽  
Diffusion Capacity ◽  
Relationship Of

scholarly journals Immunodiagnostic Significance of Anti-RA33 Autoantibodies in Saudi Patients with Rheumatoid Arthritis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jamil A. Al-Mughales
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Primary Objective ◽  
The Other ◽  
Healthy Controls ◽  
Connective Tissue Disorders ◽  
Predictive Values ◽  
Healthy Control ◽  
Controlled Clinical Study ◽  
Saudi Patients

The primary objective of this study was to evaluate and compare the immunodiagnostic significance and utility of anti-RA33 with anti-CCP, RF, and CRP in Saudi patients with rheumatoid arthritis.Methods. This was a prospective controlled clinical study conducted at King Abdul Aziz University Tertiary Medical Centre. The sera of 41 RA patients, 31 non-RA patients, and 29 healthy controls were collected. Anti-RA33 and anti-CCP were measured using commercially available ELISA principle kits. RF and CRP were measured using nephelometry.Results. Anti-RA33 antibodies had the lowest positive and negative predictive values and showed a sensitivity of 7.32% with 95.12% specificity. Of the other three markers (including anti-CCP antibodies, CRP, and RF), only anti-CCP showed specificity of 90.46% with sensitivity of 63.41% compared to non-RA patients + healthy control. There was a significant correlation with rheumatoid factor positivity with anti-CCP. With respect to CRP, a notable correlation was seen only with anti-RA33.Conclusion. Compared to rheumatoid factor, anti-CCP antibodies, and C-reactive proteins, the anti-RA33 autoantibodies seem to be not representing as an important additional immunodiagnostic marker in Saudi patients with established RA. RA33 may have more interest in early RA or less severe RA and other systemic connective tissue disorders.

scholarly journals Abberant Immunoglobulin G Glycosylation in Rheumatoid Arthritis by LTQ-ESI-MS

2020 ◽  
Vol 21 (6) ◽  
pp. 2045 ◽  
Author(s):  
Zhipeng Su ◽  
Qing Xie ◽  
Yanping Wang ◽  
Yunsen Li
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immunoglobulin G ◽  
Ion Trap ◽  
Ionization Mass ◽  
Healthy Controls ◽  
Esi Ms ◽  
Aberrant Glycosylation ◽  
Detection And Identification ◽  
Highly Sensitive

Aberrant glycosylation has been observed in many autoimmune diseases. For example, aberrant glycosylation of immunoglobulin G (IgG) has been implicated in rheumatoid arthritis (RA) pathogenesis. The aim of this study is to investigate IgG glycosylation and whether there is an association with rheumatoid factor levels in the serum of RA patients. We detected permethylated N-glycans of the IgG obtained in serum from 44 RA patients and 30 healthy controls using linear ion-trap electrospray ionization mass spectrometry (LTQ-ESI-MS), a highly sensitive and efficient approach in the detection and identification of N-glycans profiles. IgG N-glycosylation and rheumatoid factor levels were compared in healthy controls and RA patients. Our results suggested that total IgG purified from serum of RA patients shows significantly lower galactosylation (p = 0.0012), lower sialylation (p < 0.0001) and higher fucosylation (p = 0.0063) levels compared with healthy controls. We observed a positive correlation between aberrant N-glycosylation and rheumatoid factor level in the RA patients. In conclusion, we identified aberrant glycosylation of IgG in the serum of RA patients and its association with elevated levels of rheumatoid factor.

scholarly journals Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection

Medicina ◽  
2020 ◽  
Vol 56 (10) ◽  
pp. 545
Author(s):  
Khai-Jing Ng ◽  
Hui-Chun Yu ◽  
Hsien-Yu Huang Tseng ◽  
Chia-Wen Hsu ◽  
Ming-Chi Lu
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Linear Regression Analysis ◽  
Elevated Serum ◽  
Multiple Linear Regression Analysis ◽  
Healthy Controls ◽  
Active Infection ◽  
The Difference ◽  
Relationship Of ◽  
The Relationship

Background and objectives: To investigate the serum procalcitonin (PCT) levels among patients with rheumatoid arthritis (RA) without active infection compared with healthy controls and to understand the relationship of PCT with RA disease activity, and treatment received by patients. Materials and Methods: Patients aged 20 years and above with clinician-confirmed diagnosis of RA and healthy volunteers were included during regular outpatient visits, and those with active infection symptoms and signs were excluded. RA disease activity was measured using the Disease Activity Score-28 for Rheumatoid Arthritis with erythrocyte sedimentation rate (DAS28-ESR). Medications received by the patients were also recorded. Results: A total of 623 patients with RA and 87 healthy subjects were recruited in this study. The mean PCT were significantly higher in patients with RA (6.90 ± 11.81 × 10−3 ng/mL) compared with healthy controls (1.70 ± 6.12 × 10−3 ng/mL) (p < 0.001) and the difference remained statistically significant after adjusting for age and sex. In addition, multiple linear regression analysis showed that a lower rank-transformed PCT serum level was significantly correlated with the use of biologics (p = 0.017) and a high DAS28-ESR score (p = 0.028) in patients with RA. Conclusion: Patients with RA have a significantly higher serum PCT levels compared with healthy controls. The use of biologics and an active RA disease activity were associated with a lower level of PCT in patients with RA. Further investigation is required to determine the optimal cutoff value of PCT among patients with RA and its association with disease activity and biologic usage.

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