Healthcare associated infection is one of the common infection that happens in Indonesia. One form control to prevent
healthcare associated infection is the sterilization process of the materials and medical instruments that used for taking care
of patients. At the private hospital whereas a place of research, there’s never been done the study of sterility test for reusable
instrument with pouches, based on previous studies showed that 8 sets from 40 sets of reusable instrument with linen were
not sterile moreover there were positively influence from the amount of time to the sterility of reusable instrument. The
purpose of these studies was to determining the relationship between a long storage time and the sterility of reusable
instruments with pouches. The method that used in this study was the sterility testing of reusable instrument with pouches
which were stored in a central operations room storage with a long storage time of 1 and 2 months. From 30 reusable
instruments with pouches which were stored for nine months there were 5 instruments were not sterile. The results of
statistic analysis showed that the amount of storage time not significantly associated to the sterility of reusable instrument
with pouches in the operating room central storage space.