Effect of the nitrogen form used in the growth medium (NO3 −, NH4 +) on alkaloid production in Datura stramonium L.

1992 ◽  
Vol 147 (1) ◽  
pp. 79-86 ◽  
Author(s):  
K. Demeyer ◽  
R. Dejaegere
Keyword(s):  
Growth Medium ◽  
Datura Stramonium ◽  
Alkaloid Production ◽  
Nitrogen Form

Influence of minerals and elicitation on Datura stramonium L. tropane alkaloid production: Modelization of the in vitro biochemical response

Plant Science ◽  
2009 ◽  
Vol 177 (2) ◽  
pp. 81-87 ◽  
Author(s):  
R. Amdoun ◽  
L. Khelifi ◽  
M. Khelifi-Slaoui ◽  
S. Amroune ◽  
E.-H. Benyoussef ◽  
...  
Keyword(s):  
Datura Stramonium ◽  
Tropane Alkaloid ◽  
Biochemical Response ◽  
Alkaloid Production

Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium

1991 ◽  
Vol 10 (3) ◽  
Author(s):  
JudithM. Furze ◽  
MichaelJ.C. Rhodes ◽  
AdrianJ. Parr ◽  
RichardJ. Robins ◽  
IanM. Withehead ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Datura Stramonium ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Transformed Root Cultures

Alkaloid Production in Diploid and Autotetraploid Plants of Datura stramonium

2002 ◽  
Vol 40 (8) ◽  
pp. 617-621 ◽  
Author(s):  
Strahil Berkov ◽  
Stefan Philipov
Keyword(s):  
Datura Stramonium ◽  
Alkaloid Production

INTRODUCTORY STUDY FOR THE OPTIMIZATION OF GROWTH AND ALKALOID PRODUCTION BY CELL CULTURES OF DATURA STRAMONIUM L..

1992 ◽  
pp. 210-218
Author(s):  
K. Demeyer ◽  
F. Vanhaste ◽  
H. Van de Velde ◽  
R. Dejaegere
Keyword(s):  
Cell Cultures ◽  
Datura Stramonium ◽  
Alkaloid Production

The Influence of the Ca2+/K+ Balance and Light Energy on Alkaloid Content and Parttioning in Datura stramonium

1997 ◽  
Vol 45 (1) ◽  
pp. 81 ◽  
Author(s):  
K. Demeyer ◽  
R. Dejaegere
Keyword(s):  
Datura Stramonium ◽  
Light Energy ◽  
Alkaloid Content ◽  
Alkaloid Production ◽  
Light Regimes ◽  
Stage Of Development ◽  
Energy Regime ◽  
Thorn Apple

The influence of a varying Ca2+/K+ balance on alkaloid production and accumulation in Datura stramonium var. tatula L. Torr. (thorn-apple) was examined. Plants were grown in culture on two different mineral media, that varied only in the proportions of the two cations. In addition, plants were cultured under two different light regimes to investigate a possible interaction between different ecophysiological parameters. When plants were grown under a higher light energy regime, more alkaloids accumulated in the plants during a certain stage of development, but also a higher proportion was found in fruits and seeds. This was associated with a lower alkaloid content in leaves and stems. The observed effect of the Ca2+/K+ balance on alkaloid content in the leaves, was affected by the different light regimes. An interpretation, including the possible influence on glutamine content, is put forward.

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

Effect of Iron Limitation on the Ultrastructure of Agmenellum Quadruplicatum

1981 ◽  
Vol 39 ◽  
pp. 410-411
Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens
Keyword(s):  
Growth Medium ◽  
Green Alga ◽  
Natural Habitat ◽  
Iron Limitation ◽  
Natural Environments ◽  
Blue Green Alga ◽  
Marine Cyanobacterium ◽  
Natural Systems ◽  
Thin Sectioning ◽  
Agmenellum Quadruplicatum

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.

Use of multicriterial optimization of the growth medium for accumulation of biomass of lactic acid bacteria

2020 ◽  
Vol 26 (4) ◽  
pp. 47-57
Author(s):  
М. Khonkiv ◽  
S. Teterina ◽  
S. Danylenko ◽  
O. Potemska
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Medium ◽  
Multicriterial Optimization

Uso actual de Miyaya (Datura Stramonium) por los Araucanos de Chile

1960 ◽  
Vol 49 (1) ◽  
pp. 37-43
Author(s):  
Carlos A. Munizaga
Keyword(s):  
Datura Stramonium
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