Tryptophan decarboxylase from Catharanthus roseus cell suspension cultures: purification, molecular and kinetic data of the homogenous protein

1984 ◽  
Vol 3 (5) ◽  
pp. 281-288 ◽  
Author(s):  
W. No� ◽  
C. Mollenschott ◽  
J. Berlin
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Kinetic Data ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Tryptophan Decarboxylase

Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus

1981 ◽  
Vol 36 (1-2) ◽  
pp. 40-43 ◽  
Author(s):  
K.-H. Knobloch ◽  
B. Hansen ◽  
J. Berlin
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regulatory Function ◽  
Tryptophan Decarboxylase ◽  
Induction Medium ◽  
Cell Transfer ◽  
Measuring Period

Abstract Recently medium conditions have been developed which stimulate the formation of the indole alkaloid ajmalicine in cell suspension cultures of Catharanthus roseus [6]. When cells were subjected to these conditions the alkaloid accumulation was preceded by a 12-fold increase of the specific activity of tryptophan decarboxylase. The enzyme activity showed a maximum two days after the cell transfer into the induction medium and subsequently declined. In contrast the activity of strictosidine synthase, the enzyme condensing tryptamine and secologanin, was present over the entire measuring period at a constant level. The intracellular content of tryp­tamine and ajmalicine increased during a period of 6 days after cell transfer and reached a plateau after that time. A possible regulatory function of tryptophan decarboxylase in indole alkaloid biosynthesis is discussed.

scholarly journals Biotransformation of Cyclodextrine-Complexed Semisynthetic Betulin Derivatives by Plant Cells

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 743-748
Author(s):  
Suvi Häkkinen ◽  
Heli Nygren ◽  
Natalia Maiorova ◽  
Raisa Haavikko ◽  
Sami Alakurtti ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Water Soluble ◽  
Betulonic Acid ◽  
Poorly Water Soluble ◽  
Betulin Derivatives

AbstractIn this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-soluble substrates. Several new derivatives were found, consisting of oxidized and glycosylated (pentose- and hexose-conjugated) products.

Regioselective hydroxylation of warfarin by cell suspension cultures of Catharanthus roseus

1993 ◽  
Vol 33 (3) ◽  
pp. 599-600 ◽  
Author(s):  
Hiroki Hamada ◽  
Yoshihiro Fuchikami ◽  
Robert L. Jansing ◽  
Laurence S. Kaminsky
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regioselective Hydroxylation

Influence of Medium Composition on the Formation of Secondary Compounds in Cell Suspension Cultures of Catharanthus roseus (L.) G. Don

1980 ◽  
Vol 35 (7-8) ◽  
pp. 551-556 ◽  
Author(s):  
K.-H. Knobloch ◽  
J. Berlin
Keyword(s):  
Phenolic Compounds ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Culture Media ◽  
Sucrose Solution ◽  
Cell Suspension Cultures ◽  
Secondary Compounds ◽  
Suspension Cultures ◽  
Medium Composition ◽  
High Concentrations

Cell suspension cultures of Catharanthus roseus have been subjected to various media condi­tions in order to stimulate the formation of indole alkaloids. High ajmalicine contents (up to 0.5 mg/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volume of a 8% sucrose solution. The alkaloid accumulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level of phenolic compounds was found, whereas growth of the culture was low. The accumulation of both, alkaloids and poly­phenols was stimulated by high concentrations of sucrose and low concentrations of nitrogen con­taining salts and phosphate. When these minerals were added to the sucrose solution in con­centrations commonly used for cell culture media, the accumulation of alkaloids and phenolic compounds was largely suppressed.

Regulation of vinblastine biosynthesis in cell suspension cultures of catharanthus roseus

2012 ◽  
Vol 112 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Zhi-Gang Guo ◽  
Yun Liu ◽  
Mei-Zhen Gong ◽  
Wen Chen ◽  
Wen-Yuan Li
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Cell Suspension Cultures ◽  
Suspension Cultures
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