Elicitor-mediated induction of phenylalanine ammonia lyase and tryptophan decarboxylase: Accumulation of phenols and indole alkaloids in cell suspension cultures of Catharanthus roseus

1989 ◽  
Vol 18 (1) ◽  
pp. 71-78 ◽  
Author(s):  
H. U. Seitz ◽  
U. Eilert ◽  
V. De Luca ◽  
W. G. W. Kurz
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Phenylalanine Ammonia Lyase ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Tryptophan Decarboxylase

Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus

1981 ◽  
Vol 36 (1-2) ◽  
pp. 40-43 ◽  
Author(s):  
K.-H. Knobloch ◽  
B. Hansen ◽  
J. Berlin
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regulatory Function ◽  
Tryptophan Decarboxylase ◽  
Induction Medium ◽  
Cell Transfer ◽  
Measuring Period

Abstract Recently medium conditions have been developed which stimulate the formation of the indole alkaloid ajmalicine in cell suspension cultures of Catharanthus roseus [6]. When cells were subjected to these conditions the alkaloid accumulation was preceded by a 12-fold increase of the specific activity of tryptophan decarboxylase. The enzyme activity showed a maximum two days after the cell transfer into the induction medium and subsequently declined. In contrast the activity of strictosidine synthase, the enzyme condensing tryptamine and secologanin, was present over the entire measuring period at a constant level. The intracellular content of tryp­tamine and ajmalicine increased during a period of 6 days after cell transfer and reached a plateau after that time. A possible regulatory function of tryptophan decarboxylase in indole alkaloid biosynthesis is discussed.

The relationship between the accumulation of a 28 kd polypeptide and that of indole alkaloids in Catharanthus roseus cell suspension cultures

1997 ◽  
Vol 150 (4) ◽  
pp. 452-457 ◽  
Author(s):  
Sabine Carpin ◽  
Lazhar Ouelhazi ◽  
Mohamed Filali ◽  
Jean-Claude Chénieux ◽  
Marc Rideau ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
The Relationship
Sign in / Sign up

Export Citation Format

Share Document