Photochrome-mediated regulation of messenger RNAs for the small subunit of ribulose 1, 5-bisphosphate carboxylase and the light-harvesting chlorophyll a/b-protein in Lemna gibba

1981 ◽  
Vol 1 (1) ◽  
pp. 35-51 ◽  
Author(s):  
Elaine M. Tobin
Keyword(s):  
Chlorophyll A ◽  
Light Harvesting ◽  
Small Subunit ◽  
Lemna Gibba ◽  
Messenger Rnas ◽  
Bisphosphate Carboxylase ◽  
Ribulose 1

scholarly journals Light-regulated gene expression during maize leaf development.

1984 ◽  
Vol 98 (2) ◽  
pp. 558-564 ◽  
Author(s):  
T Nelson ◽  
M H Harpster ◽  
S P Mayfield ◽  
W C Taylor
Keyword(s):  
Gene Expression ◽  
Chlorophyll A ◽  
Phosphoenolpyruvate Carboxylase ◽  
Leaf Development ◽  
Light Harvesting ◽  
Messenger Rnas ◽  
Bisphosphate Carboxylase ◽  
Maize Leaf ◽  
Protein Gel ◽  
Regulated Gene Expression

We have established schedules of expression during maize leaf development in light and darkness for the messenger RNAs (mRNAs) and polypeptides for ribulose 1,5-bisphosphate carboxylase (RuBPCase) subunits, phosphoenolpyruvate carboxylase (PEPCase), and the light-harvesting chlorophyll a/b-binding protein (LHCP). Levels of mRNAs were measured by hybridization with cloned probes, and proteins were measured by immunodetection on protein gel blots. The initial synthesis in leaves of all four mRNAs follows a light-independent schedule; illumination influences only the level to which each mRNA accumulates. The synthesis of RuBPCase small and large subunits and of PEPCase polypeptides also follows a light-independent schedule which is modified quantitatively by light. However, the accumulation of LHCP polypeptides absolutely requires illumination. The accumulation of each protein closely follows the accumulation of its mRNA during growth in light. Higher ratios of PEPCase and RuBPCase protein to mRNA occur during dark growth.

Sign in / Sign up

Export Citation Format

Share Document