High frequency somatic embryogenesis and plantlet regeneration from hypocotyl protoplast cultures of Brassica napus

1995 ◽  
Vol 42 (1) ◽  
pp. 113-115 ◽  
Author(s):  
Dwarkesh S. Parihar ◽  
Satish C. Maheshwari ◽  
Paramjit Khurana
Keyword(s):  
Somatic Embryogenesis ◽  
Brassica Napus ◽  
High Frequency ◽  
Plantlet Regeneration

High-frequency plantlet regeneration by somatic embryogenesis from mature zygotic embryos of onion

2015 ◽  
Vol 43 (4) ◽  
pp. 249-260 ◽  
Author(s):  
X Wu ◽  
F Yang ◽  
XC Piao ◽  
KH Li ◽  
ML Lian ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Plantlet Regeneration ◽  
Zygotic Embryos

High frequency somatic embryogenesis, regeneration and correlation of alkaloid biosynthesis with gene expression in Papaver somniferum

2012 ◽  
Vol 68 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Sumya Pathak ◽  
Brij Kishore Mishra ◽  
Prashant Misra ◽  
Pratibha Misra ◽  
Vinod Kumar Joshi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
High Frequency ◽  
Papaver Somniferum ◽  
Alkaloid Biosynthesis

scholarly journals High frequency somatic embryogenesis through leaf explant-derived callus culture in Muscari armeniacum cv. 'Early Giant'

2012 ◽  
Vol 39 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Hyang-Bun Lee ◽  
Su-Min Jeon ◽  
Mi-Young Chung ◽  
Jeung-Sul Han ◽  
Chang-Kil Kim ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Culture ◽  
High Frequency ◽  
Leaf Explant

scholarly journals High-frequency Somatic Embryogenesis from Quiescent Seed Cotyledons of Cucumis melo Cultivars

1993 ◽  
Vol 118 (3) ◽  
pp. 425-432 ◽  
Author(s):  
D.J. Gray ◽  
D.W. McColley ◽  
Michael E. Compton
Keyword(s):  
Somatic Embryogenesis ◽  
Embryo Development ◽  
High Frequency ◽  
Somatic Embryos ◽  
Cucumis Melo ◽  
Sucrose Concentration ◽  
Male Sterile ◽  
Embryo Induction ◽  
Initial Culture ◽  
Dichlorophenoxy Acetic Acid

A protocol for high-frequency somatic embryogenesis in Cucumis melo L. was developed using `Male Sterile A147 as a model cultivar. Basal halves of quiescent seed cotyledons were cultured on embryo induction (EI) medium containing concentration ranges of the auxin 2,4-D and the cytokinins BA, Bin, TDZ, or 2iP before transfer to embryo development (ED) medium. Medium with 2,4-D at 5 mg·liter-1 and TDZ at 0.1 mg·liter-1 was superior, with 49% of explants responding and an average of 3.3 somatic embryos per explant (6.8 somatic embryos per responding explant). More explants produced embryos when incubated on EI medium for 1 or 2 weeks (30% and 33%) than for 3 or 4 weeks or with no induction. However, 2 weeks was 2.9 times better than 1 week in terms of number of embryos per explant. One week of initial culture in darkness, followed by a 16 hour light/8 hour dark photoperiod, produced more responding explants (26%) than two or more weeks in darkness or no dark period at all; but 1 and 2 weeks of darkness resulted in a similar number of embryos per explant (2.1 and 2.8). Sucrose concentration in EI and ED media had a highly significant effect on embryo induction and development. EI medium with 3% sucrose resulted in more embryogenic explants than EI medium with 1.5% or 6% sucrose. However, treatments with 3% sucrose in EI medium and 3% or 6% sucrose in ED medium produced significantly more embryos per explant (8.5 and 11.9) than other treatments. Treatments did not affect embryo induction directly and regeneration per se but, instead, frequency and efficiency of somatic embryo development. The optimal treatments were tested with 51 other commercial varieties. All varieties underwent somatic embryogenesis, exhibiting a response of 5% to 100% explant response and 0.1-20.2 embryos per explant. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine (benzyladenine or BA); N-(2-furanylmethyl)-lH-purin-6-amine (kinetin or BIN); N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron or TDZ); N-(3-methyl-2-butenyl)-lH-purin-6-amine (2iP); (2,4-dichlorophenoxy) acetic acid (2,4-D).

Sign in / Sign up

Export Citation Format

Share Document