Natural populations of Drosophila simulans show great uniformity of the mitochondrial DNA restriction map

Genetica ◽  
1988 ◽  
Vol 77 (2) ◽  
pp. 133-136 ◽  
Author(s):  
L. Nigro
1991 ◽  
Vol 57 (2) ◽  
pp. 123-126 ◽  
Author(s):  
Etsuko T. Matsuura ◽  
Haruyo Fukuda ◽  
Sadao I. Chigusa

SummaryMitochondrial DNA (mtDNA) variation in Drosophila simulans was studied to determine whether the cytoplasmic state of mtDNA heteroplasmy persists in natural populations in Réunion. For this purpose, 172 isofemale lines, newly collected from two local populations, were examined, among which three types of mtDNA (siII, siIII and siIII′) were found, based on the Hpa II restriction pattern. Ten of the lines were heteroplasmic for a combination of siII and siIII, as determined by autoradiography. The same type of heteroplasmy had been noted in one of the two local populations 8 years before (Satta et al. 1988). The present results suggest that the heteroplasmic state occurs recurrently in natural populations of D. simulans in Réunion.


1994 ◽  
Vol 40 (8) ◽  
pp. 612-621 ◽  
Author(s):  
János Varga ◽  
Ferenc Kevei ◽  
Aldwin Vriesema ◽  
Fons Debets ◽  
Zofia Kozakiewicz ◽  
...  

The mitochondrial DNAs (mtDNAs) and the ribosomal repeat unit (ribosomal DNA, rDNA) of black Aspergillus isolates collected in various parts of the world were examined. Wide-ranging mtDNA variation was observed in natural populations of the Aspergillus niger aggregate. Most isolates were classifiable as A. niger or Aspergillus tubingensis according to their rDNA and mtDNA patterns. The mtDNA variation was distributed unevenly in the populations studied. The mtDNAs of most of the isolates collected in Australia were of the A. tubingensis type, with an unexpectedly high degree of variation, while the rDNA of these isolates exhibited the same A. tubingensis pattern as that of isolates from other locations. Some other local populations displayed very little polymorphism in their mtDNA and rDNA. Hybridization experiments in which cloned A. niger and Aspergillus nidulans mtDNA fragments were used revealed that the two main mtDNA groups corresponding to A. niger and A. tubingensis are more distantly related than concluded earlier. Six of the 13 Brazilian isolates examined exhibited mtDNA and rDNA types different from those of all the other strains and could not be classified into the above species. Classical taxonomic examination of these strains is in progress.Key words: Aspergillus niger aggregate, mitochondrial DNA, RFLP.


Genome ◽  
1989 ◽  
Vol 32 (2) ◽  
pp. 340-342 ◽  
Author(s):  
William S. Davidson ◽  
Tim P. Birt ◽  
John M. Green

A restriction map of Atlantic salmon mitochondrial DNA was constructed. The smallest XbaI fragment of the salmon mitochondrial genome was cloned and subjected to partial DNA sequence analysis. This fragment contains the genes for ATPase 6 and cytochrome oxidase III. The putative organisation of the mitochondrial genome relative to the physical map is shown.Key words: salmonid, mitochondrial DNA, restriction map, sequence analysis.


1994 ◽  
Vol 32 (1-2) ◽  
pp. 25-33 ◽  
Author(s):  
Bruce A. McPheron ◽  
Gail E. Gasparich ◽  
Ho-Yeon Han ◽  
Gary J. Steck ◽  
Walter S. Sheppard

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