Lipase activity of thermophilic bacteria from icelandic hot springs

1993 ◽  
Vol 15 (4) ◽  
pp. 361-366 ◽  
Author(s):  
Sj�fn Sigurg�slad�ttir ◽  
Malta Konr��sd�ttir ◽  
�sbj�rn J�nsson ◽  
Jakob K. Kristj�nsson ◽  
Einar Matthiasson
2017 ◽  
Vol 8 ◽  
pp. 19-28
Author(s):  
ASHISH DHYANI ◽  
RITU GURURANI ◽  
SAMY A. SELIM ◽  
PRIYANKA ADHIKARI ◽  
AVINASH SHARMA ◽  
...  

Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.


2011 ◽  
Vol 22 ◽  
pp. S88
Author(s):  
Kadriye İnan ◽  
Müslüm Tokgöz ◽  
Sabriye Çanakçi ◽  
Ali Osman Beldüz

2018 ◽  
Vol 44 (1) ◽  
pp. 75-85
Author(s):  
S. A. Alrumman ◽  
Y. S. Mostafa ◽  
Shekha T. S. Al-Qahtani ◽  
T. Sahlabji ◽  
T. H. Taha

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Kenny Lischer ◽  
ANANDA BAGUS RICHKY DIGDAYA PUTRA ◽  
Brian Wirawan Guslianto ◽  
Forbes Avila ◽  
Sarah Grace Sitorus ◽  
...  

Abstract. Lischer K, Putra ABRD, Guslianto BW, Avilla F, Sitorus SG, Nugraha Y, Sarmoko. 2020. Short Communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia. Biodiversitas 21: 5474-5481. Indonesia is an archipelagic country located in the pacific ring of fire, and is estimated to cause numerous hot springs spread across the country. In addition, small living microbes have been explored in these locations since 1985. These microbes possess the ability to survive in areas with high temperature (more than 40oC-90oC), and are therefore termed thermophiles. Hence, massive explorations have been conducted on Java island and other unexplored areas at Sumatra to Papua in New Guinea islands. Moreover, a total of 71 hot springs characterized by the presence of thermophilic bacteria have been explored in Indonesia. These investigations ensue with various approaches, including through conventional and microbiological, 16S rRNA, as well as whole-genome sequencing methods. In addition to species exploration, the application of thermophiles has become a topic of interest from 1999, especially based on thermostable enzymes with the capacity to maintain activity at high-temperature conditions. These include amylase, protease, lipase, xylanase, esterase, and cellulase as the most common isolated form, which indicates the existence of significant extractable potentials. Hence, there is a need for further research in terms of both exploration and application purposes.


2009 ◽  
Vol 79 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Ahmet Adiguzel ◽  
Hakan Ozkan ◽  
Ozlem Baris ◽  
Kadriye Inan ◽  
Medine Gulluce ◽  
...  

1972 ◽  
Vol 36 (13) ◽  
pp. 2357-2366 ◽  
Author(s):  
Takashi SAIKI ◽  
Ryohei KIMURA ◽  
Kei ARIMA

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