Purification and characterization of N-carbomoyl-l-amino acid amidohydrolase with broad substrate specificity from Alcaligenes xylosoxidans

1995 ◽  
Vol 43 (6) ◽  
pp. 1039-1043 ◽  
Author(s):  
J. Ogawa ◽  
H. Miyake ◽  
S. Shimizu
Keyword(s):  
Amino Acid ◽  
Substrate Specificity ◽  
Purification And Characterization ◽  
Broad Substrate Specificity ◽  
Acid Amidohydrolase

Purification and characterization of a post-proline dipeptidyl aminopeptidase fromStreptococcus cremorisAM2

1990 ◽  
Vol 57 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Mary Booth ◽  
Ide Ni Fhaoláin ◽  
P. Vincent Jennings ◽  
Gerard O'Cuinn
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Substrate Specificity ◽  
Purification And Characterization ◽  
Ph Optimum ◽  
Cheese Ripening ◽  
Streptococcus Cremoris ◽  
Scissile Bond ◽  
Dipeptidyl Aminopeptidase

SummaryThe present study describes the purification of a post-proline dipeptidyl aminopeptidase from the cytoplasm ofStreptococcus cremorisAM2. On the basis of its elution from a calibrated Sephadex G200 column, the enzyme had a molecular weight of 117000 and exhibited a broad pH optimum activity between 6·0 and 9·0. The activity was most comprehensively inhibited by phenylmethylsulphonylfluoride and more modestly inhibited by 1,10-phenanthroline and 8-hydroxyquinoline but not by EDTA. A range of peptides containing either proline or alanine as the penultimate amino acid residue could act as substrates. The presence of proline on the carboxy side of the scissile bond prevented hydrolysis. However the enzyme could release Pro-Pro from Pro-Pro-Gly-Phe-Ser-Pro. The significance of this substrate specificity is considered in the context of removal of either single proline residues or prolylproline sequences from oligopeptides during cheese ripening.

scholarly journals Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells

Plant Science ◽  
2000 ◽  
Vol 157 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Goro Taguchi ◽  
Hirofumi Imura ◽  
Yoshio Maeda ◽  
Ritsuko Kodaira ◽  
Nobuaki Hayashida ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cultured Cells ◽  
Purification And Characterization ◽  
Broad Substrate Specificity
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