Identification of fetal hemoglobin in blood stains by high performance liquid chromatography

1989 ◽  
Vol 102 (7) ◽  
Author(s):  
H. Inoue ◽  
F. Takabe ◽  
Y. Maeno ◽  
M. Iwasa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Blood Stains

Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography

1991 ◽  
Vol 104 (3) ◽  
pp. 127-131 ◽  
Author(s):  
Hiroyuki Inoue ◽  
Fukutaro Takabe ◽  
Mineo Iwasa ◽  
Yoshitaka Maeno
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Simultaneous Estimation

Comparison of an Isoelectric Focusing Technique and High-Performance Liquid Chromatography for Determination of Fetal Hemoglobin Levels

1991 ◽  
Vol 96 (1) ◽  
pp. 109-110 ◽  
Author(s):  
Enid F. Gilbert-Barness ◽  
Katherine S. Kenison ◽  
Earl Shrago ◽  
Terry L. Spennetta ◽  
Gary G. Giulian
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Fetal Hemoglobin

The analysis of romanowsky blood stains by high-performance liquid chromatography

1976 ◽  
Vol 124 (2) ◽  
pp. 287-301 ◽  
Author(s):  
W.W. Dean ◽  
G.J. Lubrano ◽  
H.G. Heinsohn ◽  
M. Stastny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Blood Stains

scholarly journals Transfusion associated peak in Hb HPLC chromatogram – a case report

2012 ◽  
Vol 4 (1) ◽  
pp. e2012006
Author(s):  
Sonal Jain ◽  
Jasmita Dass ◽  
Hara Prasad Pati
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Case Report ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Juvenile Myelomonocytic Leukemia ◽  
Insufficient Information ◽  
Hplc Chromatogram ◽  
Myelomonocytic Leukemia ◽  
Transfusion History

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.

Monoclonal antibody to the gamma chain of human fetal hemoglobin used to develop an enzyme immunoassay.

1989 ◽  
Vol 35 (10) ◽  
pp. 2066-2069 ◽  
Author(s):  
H Moscoso ◽  
M Shyamala ◽  
C R Kiefer ◽  
F A Garver
Keyword(s):  
Monoclonal Antibody ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Gamma Chain ◽  
Cell Hybridization ◽  
Microtiter Plates ◽  
Beta 2 ◽  
Hb Bart's

Abstract A monoclonal antibody (mAb) that recognizes the gamma chain of human fetal hemoglobin (Hb F) has been produced by cell hybridization techniques. The mAb reacts with Hb F (alpha 2 gamma 2), Hb Bart's (gamma 4), and Hb Kenya (gamma-beta hybrid), but does not cross-react with Hb A (alpha 2 beta 2) or Hb A2 (alpha 2 delta 2). We describe a direct enzyme-linked immunoassay (ELISA) for measurement of Hb F, in which hemoglobins from standards or from unknown hemolysates are covalently bound to the wells of microtiter plates. The antigen is quantified by addition of the gamma-specific mAb, followed by anti-mouse IgG conjugated with horseradish peroxidase, and incubation with the substrate, tetramethylbenzidine. Absorbances at 630 nm are directly proportional to the amount of Hb F present in the standards or samples. Results for Hb F in 53 hemolysates agreed well with values obtained by "high-performance" liquid chromatography, RIA, alkali denaturation, and magnetic affinity immunoassay. This ELISA can detect a 0.5% proportion of Hb F in 1 h and offers distinct advantages over other techniques currently in use.

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