scholarly journals Transfusion associated peak in Hb HPLC chromatogram – a case report

2012 ◽  
Vol 4 (1) ◽  
pp. e2012006
Author(s):  
Sonal Jain ◽  
Jasmita Dass ◽  
Hara Prasad Pati
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Case Report ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Juvenile Myelomonocytic Leukemia ◽  
Insufficient Information ◽  
Hplc Chromatogram ◽  
Myelomonocytic Leukemia ◽  
Transfusion History

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.

Fixed eruption due to quinine in tonic water: A case report with high-performance liquid chromatography and ultraviolet A analyses

2013 ◽  
Vol 40 (8) ◽  
pp. 629-631 ◽  
Author(s):  
Aoi Ohira ◽  
Sayaka Yamaguchi ◽  
Takuya Miyagi ◽  
Yu-ichi Yamamoto ◽  
Satoshi Yamada ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Case Report ◽  
High Performance ◽  
Ultraviolet A ◽  
Tonic Water

Comparison of an Isoelectric Focusing Technique and High-Performance Liquid Chromatography for Determination of Fetal Hemoglobin Levels

1991 ◽  
Vol 96 (1) ◽  
pp. 109-110 ◽  
Author(s):  
Enid F. Gilbert-Barness ◽  
Katherine S. Kenison ◽  
Earl Shrago ◽  
Terry L. Spennetta ◽  
Gary G. Giulian
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Fetal Hemoglobin

Analysis and Identification of Astaxanthin and its Carotenoid Precursors from Xanthophyllomyces dendrorhous by High-Performance Liquid Chromatography

2010 ◽  
Vol 65 (7-8) ◽  
pp. 489-494 ◽  
Author(s):  
Mingbo Lu ◽  
Yang’e Zhang ◽  
Chunfang Zhao ◽  
Pengpeng Zhou ◽  
Longjiang Yu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Absorption Spectra ◽  
Mobile Phase ◽  
High Performance ◽  
Biosynthetic Pathway ◽  
Hplc Method ◽  
Xanthophyllomyces Dendrorhous ◽  
Hplc Chromatogram ◽  
Carotenoid Pathway

This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and UV-vis absorption spectra. Peaks showed in the HPLC chromatogram are identified as carotenoids in the monocyclic biosynthetic pathway or their derivatives. In the monocyclic carotenoid pathway, 3,3’-dihydroxy- β,ψ-carotene-4,4’-dione (DCD) is produced through γ-carotene and torulene.

scholarly journals COLCHICINA: INTOXICAÇÃO E ÓBITO, RELATO DE CASO

2004 ◽  
Vol 5 (1) ◽  
Author(s):  
P.M.K.C. GABRIEL ◽  
M.M. GABRIEL ◽  
J. ESCORSIN NETO ◽  
M. LOPES
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Case Report ◽  
High Performance ◽  
High Concentration ◽  
Emergency Ward ◽  
Death Case ◽  
Analytical Results ◽  
Mean Concentration

B. I., de 67 anos, foi internado em um hospital no interior do Estado do Paraná, apresentando dor abdominal, vômitos e diarréia intensa, vindo a falecer após 4h de internamento. Após investigação do médico sanitarista, constatou-se que a vítima havia começado tratamento com cápsulas manipuladas com o princípio ativo “colchicina”. Análise por cromatografia líquida de alta resolução (HPLC) indicou positividade para colchicina nas cápsulas, com concentração média de 63,48mg e negativo no material biológico da vítima. A intoxicação por colchicina foi confirmada através dos exames anátomo patológico, sintomatologia apresentada no período de internamento e a alta dosagem de colchicina encontrada nas cápsulas manipuladas e ainda disponíveis. COLCHICINE: INTOXICATION AND DEATH, CASE REPORT Abstract B. I., sixty-seven years old, was taken to a hospital with abdominal cramps, vomiting and intense diarrhoea; he died four hours after being admitted in the emergency ward. After some medical investigation, it became known that the patient had started a treatment with colchicine in capsules prepared by a pharmacist. The capsules and the autopsy materials were analysed by High Performance Liquid Chromatography (HPLC) and the analytical results for the capsules were positive for colchicine with mean concentration of 63,48mg/capsule; the analytical results for the autopsy materials were negative for colchicine. The fatal colchicine intoxication was confirmed by the anatomo-pathological exams carried out in the autopsy materials, by the sintomatology presented by the patient during his staying in the hospital and by the high concentration of colchicine found in the still remaining capsules.

Monoclonal antibody to the gamma chain of human fetal hemoglobin used to develop an enzyme immunoassay.

1989 ◽  
Vol 35 (10) ◽  
pp. 2066-2069 ◽  
Author(s):  
H Moscoso ◽  
M Shyamala ◽  
C R Kiefer ◽  
F A Garver
Keyword(s):  
Monoclonal Antibody ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fetal Hemoglobin ◽  
Gamma Chain ◽  
Cell Hybridization ◽  
Microtiter Plates ◽  
Beta 2 ◽  
Hb Bart's

Abstract A monoclonal antibody (mAb) that recognizes the gamma chain of human fetal hemoglobin (Hb F) has been produced by cell hybridization techniques. The mAb reacts with Hb F (alpha 2 gamma 2), Hb Bart's (gamma 4), and Hb Kenya (gamma-beta hybrid), but does not cross-react with Hb A (alpha 2 beta 2) or Hb A2 (alpha 2 delta 2). We describe a direct enzyme-linked immunoassay (ELISA) for measurement of Hb F, in which hemoglobins from standards or from unknown hemolysates are covalently bound to the wells of microtiter plates. The antigen is quantified by addition of the gamma-specific mAb, followed by anti-mouse IgG conjugated with horseradish peroxidase, and incubation with the substrate, tetramethylbenzidine. Absorbances at 630 nm are directly proportional to the amount of Hb F present in the standards or samples. Results for Hb F in 53 hemolysates agreed well with values obtained by "high-performance" liquid chromatography, RIA, alkali denaturation, and magnetic affinity immunoassay. This ELISA can detect a 0.5% proportion of Hb F in 1 h and offers distinct advantages over other techniques currently in use.

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