Identification of novel human T-cell receptor V? gene segments by the anchored-polymerase chain reaction

1992 ◽  
Vol 36 (5) ◽  
Author(s):  
Claudio Lunardi ◽  
Christopher Marguerie ◽  
AlexK. So
Keyword(s):  
Polymerase Chain Reaction ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Reaction ◽  
Human T Cell ◽  
Polymerase Chain ◽  
V Gene

The use of anchored polymerase chain reaction for the study of large numbers of human T-cell receptor transcripts

1993 ◽  
Vol 30 (13) ◽  
pp. 1143-1150 ◽  
Author(s):  
Laurent Ferradini ◽  
Sergio Roman-Roman ◽  
Orly Azogui ◽  
Catherine Geneve´e ◽  
Sophie Viel ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Reaction ◽  
Human T Cell ◽  
Large Numbers ◽  
Polymerase Chain

scholarly journals Detection of T-cell receptor gamma chain V gene rearrangements using the polymerase chain reaction: application to the study of clonal disease cells in acute lymphoblastic leukemia

Blood ◽  
1991 ◽  
Vol 77 (9) ◽  
pp. 1989-1995 ◽  
Author(s):  
JJ Taylor ◽  
D Rowe ◽  
IK Williamson ◽  
SE Christmas ◽  
SJ Proctor ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
T Cell ◽  
T Cell Receptor ◽  
Lymphoblastic Leukemia ◽  
Cell Receptor ◽  
Chain Reaction ◽  
Gamma Chain ◽  
Cell Clones ◽  
Polymerase Chain

Abstract This report describes the development and characterization of a method for the amplification of rearranged V-J segments of the human T-cell receptor gamma chain (TCRG) locus using an adaptation of the polymerase chain reaction (PCR) technique. The technique uses a single pair of ‘consensus’ primers to amplify rearrangements involving the V gamma I subgroup genes, which are common in malignant cells from acute lymphoblastic leukemia (ALL) patients. Using this method we were able to detect rearrangements in the TCRG locus in disease cells from patients with T-cell ALL (12 of 12), common ALL (10 of 14), and Null cell ALL (2 of 2) at presentation. Monoallelic and biallelic rearrangements involving V gamma I subgroup genes were identified by restriction analysis of PCR products from DNA samples from a T-cell leukemic cell line, T-cell clones, and disease cells from patients with ALL of T-and B-cell lineage at presentation. These results confirmed the presence of cell clones within the presentation samples and, in one case, confirmed the persistence of the original malignant cell clone at relapse. This is a rapid and specific method for the detection and characterization of rearrangements of the TCRG locus without recourse to Southern blotting. Therefore, the PCR technique described herein can provide the basis for the study of clonal evolution and minimal residual disease on a high proportion of patients with ALL.

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