Electron-microscopic demonstration of olfactory-marker protein with protein G-gold in freeze-substituted, Lowicryl K11M-embedded rat olfactory-receptor cells

1989 ◽  
Vol 256 (2) ◽  
Author(s):  
BertPh.M. Menco
Keyword(s):  
Olfactory Receptor ◽  
Protein G ◽  
Marker Protein ◽  
Olfactory Marker Protein ◽  
Electron Microscopic ◽  
Olfactory Receptor Cells ◽  
Receptor Cells ◽  
Microscopic Demonstration ◽  
Electron Microscopic Demonstration

Analysis for Olfactory Epithelium using Olfactory Marker Protein on Endoscopically Harvested Middle Turbinates

1996 ◽  
Vol 10 (4) ◽  
pp. 221-224 ◽  
Author(s):  
John F. Biedlingmaier ◽  
Philip J. Whelan
Keyword(s):  
Olfactory Epithelium ◽  
Middle Turbinate ◽  
Sinus Surgery ◽  
Marker Protein ◽  
Olfactory Marker Protein ◽  
Olfactory Cleft ◽  
Olfactory Receptor Cells ◽  
Receptor Cells ◽  
Olfactory Tissue ◽  
Positive Controls

The middle turbinate is thought to play a key role in olfaction, and many surgeons have cautioned against removal of the middle turbinate during endoscopic sinus surgery. We reviewed 110 patients having 198 partial middle turbinate resections and found that only one patient complained of postoperative anosmia (0.9%). To further investigate the presence of olfactory tissue on the middle turbinate, 36 sections from 12 endoscopically resected turbinate specimens were stained for olfactory tissue, using olfactory marker protein (OMP). Cadaveric olfactory cleft specimens served as positive controls. Neither olfactory epithelium nor olfactory receptor cells were identified in the surgical specimens. The clinical rarity of anosmia suggests that partial resections must not adversely affect airflow to the olfactory cleft. The histologic data suggest that conservative partial turbinate resections should not affect olfaction directly, either because olfactory tissue is not removed by this maneuver, or because the amount of olfactory tissue in this segment is minimal.

Ultrastructural aspects of olfactory signal transduction and its development

1994 ◽  
Vol 52 ◽  
pp. 142-143
Author(s):  
Bert Ph. M. Menco
Keyword(s):  
Signal Transduction ◽  
Olfactory Receptor ◽  
Receptor Cell ◽  
Freeze Substitution ◽  
Luminal Surface ◽  
Tissue Sections ◽  
Olfactory Receptor Cells ◽  
Receptor Cells ◽  
Olfactory Signal ◽  
Odor Molecule

Vertebrate olfactory receptor cells are specialized neurons that have numerous long tapering cilia. The distal parts of these cilia line the interface between the external odorous environment and the luminal surface of the olfactory epithelium. The length and number of these cilia results in a large surface area that presumably increases the chance that an odor molecule will meet a receptor cell. Advanced methods of cryoprepration and immuno-gold labeling were particularly useful to preserve the delicate ultrastructural and immunocytochemical features of olfactory cilia required for localization of molecules involved in olfactory signal-transduction. We subjected olfactory tissues to freeze-substitution in acetone (unfixed tissues) or methanol (fixed tissues) followed by low temperature embedding in Lowicryl K11M for that purpose. Tissue sections were immunoreacted with several antibodies against proteins that are presumably important in olfactory signal-transduction.

scholarly journals Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues

PLoS ONE ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. e0116097 ◽  
Author(s):  
NaNa Kang ◽  
Hyerin Kim ◽  
YoonGyu Jae ◽  
NaHye Lee ◽  
Cheol Ryong Ku ◽  
...  
Keyword(s):  
Protein Expression ◽  
Olfactory Receptor ◽  
Marker Protein ◽  
Olfactory Marker Protein
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