A new approach for mapping quantitative trait loci using complete genetic marker linkage maps

1994 ◽  
Vol 89 (5) ◽  
pp. 535-539 ◽  
Author(s):  
W. R. Wu ◽  
W. M. Li
2003 ◽  
Vol 128 (4) ◽  
pp. 508-514 ◽  
Author(s):  
Courtney A. Weber ◽  
Gloria A. Moore ◽  
Zhanao Deng ◽  
Fred G. Gmitter

Mapping quantitative trait loci (QTL) associated with freeze tolerance was accomplished using a Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. F1 pseudo-testcross population. A progeny population of 442 plants was acclimated and exposed to temperatures of -9 °C and -15 °C in two separate freeze tests. A subpopulation of 99 progeny was genotyped for random amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR), and sequence tagged site (STS) markers to produce a linkage map for each parent. Potential QTL were identified by interval mapping, and their validity was corroborated with results from means comparison (t test), one-way analysis of variance (F test), and bulked segregant analysis (BSA). Multiple analytical methods provided evidence supporting putative QTL and decreased the probability of missing significant QTL associated with freeze tolerance. QTL with a large effect on freeze tolerance were located on both the Citrus and Poncirus linkage maps. In addition, clusters of markers with significantly different means between marker present and absent classes indicating minor QTL that contribute smaller effects on the level of tolerance were found on the linkage maps of both species.


Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1439-1444
Author(s):  
Long-Xi Yu ◽  
Fan Zhang ◽  
Cesar Medina Culma ◽  
Sen Lin ◽  
Yi Niu ◽  
...  

Verticillium wilt (VW) of alfalfa is a devastating disease that causes forage yield reductions of up to 50% in the northern United States and Canada. The most effective method for controlling the disease is through the development and use of resistant varieties. To identify quantitative trait loci (QTL) for VW resistance in alfalfa, we used a full-sib population segregating for VW resistance. High-density linkage maps for both resistant and susceptible parents were constructed using single-dose alleles of single-nucleotide polymorphism markers generated by genotyping-by-sequencing. Five QTL associated with VW resistance were identified and they were in four linkage groups (4D, 6B, 6D, and 8C). Of those, three QTL (qVW-6D-1, qVW-6D-2, and qVW-8C) had higher logarithm of odds. Two putative candidates of nucleotide-binding site leucine-rich repeat disease resistance genes were identified in the QTL intervals of qVW-6D-2 and qVW-8C, respectively. The result agreed with our previous studies, in which similar resistance loci were identified in an association panel using genome-wide association. The results provide insight into the quantitative resistance to VW in alfalfa. The resistance loci and closely linked markers identified in the present study can be used in developing new alfalfa varieties with enhanced resistance to VW.


2010 ◽  
Vol 7 (1) ◽  
pp. 153-167 ◽  
Author(s):  
Flavia M. Moreira ◽  
Alberto Madini ◽  
Rosanna Marino ◽  
Luca Zulini ◽  
Marco Stefanini ◽  
...  

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Krishna K. Gali ◽  
Yong Liu ◽  
Anoop Sindhu ◽  
Marwan Diapari ◽  
Arun S. K. Shunmugam ◽  
...  

1990 ◽  
Vol 79 (5) ◽  
pp. 583-592 ◽  
Author(s):  
S. J. Knapp ◽  
W. C. Bridges ◽  
D. Birkes

2012 ◽  
Vol 50 (08) ◽  
Author(s):  
R Hall ◽  
R Müllenbach ◽  
S Huss ◽  
R Alberts ◽  
K Schughart ◽  
...  

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