PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5′ -anchored simple sequence repeat (SSR) primers

1996 ◽  
Vol 92-92 (3-4) ◽  
pp. 442-447 ◽  
Author(s):  
Y. M. Charters ◽  
A. Robertson ◽  
M. J. Wilkinson ◽  
G. Ramsay
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Simple Sequence Repeat ◽  
Pcr Analysis ◽  
Sequence Repeat ◽  
Brassica Napus L ◽  
Ssr Primers ◽  
Simple Sequence

PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5?-anchored simple sequence repeat (SSR) primers

1996 ◽  
Vol 92 (3-4) ◽  
pp. 442-447 ◽  
Author(s):  
Y. M. Charters ◽  
A. Robertson ◽  
M. J. Wilkinson ◽  
G. Ramsay
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Simple Sequence Repeat ◽  
Pcr Analysis ◽  
Sequence Repeat ◽  
Brassica Napus L ◽  
Ssr Primers ◽  
Simple Sequence

The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties

2003 ◽  
Vol 106 (6) ◽  
pp. 1091-1101 ◽  
Author(s):  
L. Tommasini ◽  
J. Batley ◽  
G. Arnold ◽  
R. Cooke ◽  
P. Donini ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Stability Testing ◽  
Sequence Repeat ◽  
Brassica Napus L ◽  
Simple Sequence

Divergent patterns of allelic diversity from similar origins: the case of oilseed rape (Brassica napus L.) in China and Australia

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 1-10 ◽  
Author(s):  
S. Chen ◽  
M. N. Nelson ◽  
K. Ghamkhar ◽  
T. Fu ◽  
W. A. Cowling
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Simple Sequence Repeat ◽  
Allelic Diversity ◽  
Brassica Napus L ◽  
Chinese Germplasm ◽  
Interspecific Crossing ◽  
Chinese Group ◽  
Simple Sequence ◽  
Private Alleles

Oilseed rape ( Brassica napus ) in Australia and China have similar origins, with introductions from Europe, Canada, and Japan in the mid 20th century, and there has been some interchange of germplasm between China and Australia since that time. Allelic diversity of 72 B. napus genotypes representing contemporary germplasm in Australia and China, including samples from India, Europe, and Canada, was characterized by 55 polymorphic simple sequence repeat (SSR) markers spanning the entire B. napus genome. Hierarchical clustering and two-dimensional multidimensional scaling identified a Chinese group (China-1) that was separated from a “mixed” group of Australian, Chinese (China-2), European, and Canadian lines. A small group from India was distinctly separated from all other B. napus genotypes. Chinese genotypes, especially in the China-1 group, have inherited unique alleles from interspecific crossing, primarily with B. rapa, and the China-2 group has many alleles in common with Australian genotypes. The concept of “private alleles” is introduced to describe both the greater genetic diversity and the genetic distinctiveness of Chinese germplasm, compared with Australian germplasm, after 50 years of breeding from similar origins.

scholarly journals Genetic Relationships among 10 Prunus Rootstock Species from China, Based on Simple Sequence Repeat Markers

2016 ◽  
Vol 141 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Qijing Zhang ◽  
Dajun Gu
Keyword(s):  
Phylogenetic Analysis ◽  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Banding Patterns ◽  
Simple Sequence Repeat Markers ◽  
Ssr Primers ◽  
Relationship Of ◽  
Simple Sequence

To improve the efficiency of breeding programs for Prunus rootstock hybrids in China, we analyzed the subgenus status and relationship of 10 Chinese rootstock species, by using 24 sets of simple sequence repeat (SSR) primers. The SSR banding patterns and phylogenetic analysis indicated that subgenus Cerasus is more closely related to subgenus Prunophora than to subgenus Amygdalus, and that subgenus Lithocerasus is more closely related to subgenus Prunophora and subgenus Amygdalus than to subgenus Cerasus. In addition, Prunus triloba was more closely related to Prunus tomentosa than to the members of subgenus Amygdalus. Therefore, we suggest that P. tomentosa and P. triloba should be assigned to the same group, either to subgenus Lithocerasus or Prunophora, and we also propose potential parent combinations for future Prunus rootstock breeding.

Genetic diversity of Canadian elite summer rape (Brassica napus L.) cultivars from the pre- to post-canola quality era

2010 ◽  
Vol 90 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Y -B. Fu ◽  
R K Gugel
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Gene Pools ◽  
Sequence Repeat ◽  
Low Erucic Acid ◽  
Simple Sequence ◽  
Canola Quality

The development of canola quality Brassica napus oilseed cultivars was a major achievement of Canadian public oilseed breeding programs. Simple sequence repeat (SSR) markers were applied to assess the genetic diversity of 300 plants representing one landrace introduced from Argentina in 1943, seven Canadian elite cultivars developed and released by Agriculture and Agri-Food Canada since 1954, and two European cultivars that were the source of the low erucic acid and low glucosinolate traits that define canola quality. Application of 22 SSR primer pairs from eight linkage groups detected 88 polymorphic alleles from 33 likely loci. The allelic frequencies in 300 samples ranged from 0.003 to 0.993 and averaged 0.388. The estimates of mean heterozygosity for these cultivars ranged from 0.055 to 0.203 and averaged 0.139. The most SSR variation was detected in the cultivars Argentine, Golden and Oro. A trend of decline in SSR variation was observed over the years of breeding effort. The proportion of total SSR variation residing among the cultivars was 51.4%; between high vs. low erucic acid cultivars 15% and between high vs. low glucosinolate cultivars 21.2%. Pairwise genetic differentiations among these cultivars ranged from 0.140 to 0.819 and averaged 0.500. Cluster analysis revealed that the genetic relationships of these cultivars were consistent with their known pedigrees. These findings are useful for broadening the genetic base of improved B. napus gene pools, selecting genetically diverse genotypes for hybrid combinations, and conserving summer rape germplasm.Key words: Simple sequence repeat, summer rape, Brassica napus, genetic diversity, genetic relationship, genetic structure

Identification and characterization of simple sequence repeat markers from Brassica napus expressed sequences

2007 ◽  
Vol 7 (5) ◽  
pp. 886-889 ◽  
Author(s):  
JACQUELINE BATLEY ◽  
CLARE J. HOPKINS ◽  
NOEL O. I. COGAN ◽  
MELANIE HAND ◽  
ERICA JEWELL ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Identification And Characterization ◽  
Simple Sequence

Genomic instability in sporadic colorectal cancer quantitated by inter-simple sequence repeat PCR analysis

1997 ◽  
Vol 18 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Mark Basik ◽  
Daniel L. Stoler ◽  
Konstantinos C. Kontzoglou ◽  
Miguel A. Rodriguez-Bigas ◽  
Nicholas J. Petrelli ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Genomic Instability ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Pcr Analysis ◽  
Sporadic Colorectal Cancer ◽  
Sequence Repeat ◽  
Simple Sequence
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