Behaviour of chromosomes in anaphase cells in embryogenic callus cultures of maize (Zea mays L.)

1996 ◽  
Vol 92 (8) ◽  
pp. 982-990 ◽  
Author(s):  
A. Fluminhan ◽  
T. Kameya
Keyword(s):  
Zea Mays ◽  
Embryogenic Callus ◽  
Zea Mays L ◽  
Callus Cultures

Isozymes as biochemical and cytochemical markers in embryogenic callus cultures of maize (Zea mays L.)

1989 ◽  
Vol 8 (2) ◽  
pp. 67-70 ◽  
Author(s):  
P. F. Fransz ◽  
N. C. A. de Ruijter ◽  
J. H. N. Schel
Keyword(s):  
Zea Mays ◽  
Embryogenic Callus ◽  
Zea Mays L ◽  
Callus Cultures ◽  
Cytochemical Markers

Behaviour of chromosomes in anaphase cells in embryogenic callus cultures of maize (Zea mays L.)

1996 ◽  
Vol 92 (8) ◽  
pp. 982-990 ◽  
Author(s):  
A. Fluminhan ◽  
T. Kameya
Keyword(s):  
Zea Mays ◽  
Embryogenic Callus ◽  
Zea Mays L ◽  
Callus Cultures

Regeneration of Zea mays L. from Embryogenic Callus

1985 ◽  
Vol 146 (3) ◽  
pp. 327-334 ◽  
Author(s):  
Kathryn K. Kamo ◽  
Michael R. Becwar ◽  
Thomas K. Hodges
Keyword(s):  
Zea Mays ◽  
Embryogenic Callus ◽  
Zea Mays L

An ultrastructural study on the early development of Zea mays somatic embryos

1991 ◽  
Vol 69 (4) ◽  
pp. 858-865 ◽  
Author(s):  
P. F. Fransz ◽  
J. H. N. Schel
Keyword(s):  
Zea Mays ◽  
Early Development ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Zea Mays L ◽  
Light And Electron Microscopy ◽  
Apical Region ◽  
Basal Region ◽  
Embryogenic Cells

Friable embryogenic callus, obtained from immature embryos of Zea mays L., was cultured on N6 medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, 6 mM proline, and 2% sucrose. Cultured tissue fragments containing several globular embryoids were excised and examined by light and electron microscopy to follow the early development of maize embryoids. The somatic embryos consist of an apical region and a suspensor region. Cells of the apical region are small, cytoplasm rich, and mitotically active. They contain much starch and numerous bundles of microtubules. Suspensor cells are larger and more vacuolated. A high metabolic activity in both cell types is indicated by the presence of many organelles, coated vesicles, and multivesicular bodies. Transition units appear to form intermediate stages between the embryogenic callus cells and the somatic embryo. A transition unit consists of a group of embryogenic cells and shows an apical and a basal region. The unit has many intercellular spaces, and within the cells areas with organelle-free cytosol are frequently observed. Key words: somatic embryogenesis, in vitro culture, ultrastructure, Zea mays L.

SOMATIC EMBRYOGENESIS IN LONG-TERM CALLUS CULTURES OF ZEA MAYS L. (GRAMINEAE)

1984 ◽  
Vol 71 (1) ◽  
pp. 158-161 ◽  
Author(s):  
Vimla Vasil ◽  
Indra K. Vasil ◽  
Chin-yi Lu
Keyword(s):  
Zea Mays ◽  
Somatic Embryogenesis ◽  
Zea Mays L ◽  
Callus Cultures

The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.) germplasm

1985 ◽  
Vol 70 (5) ◽  
pp. 505-509 ◽  
Author(s):  
D. T. Tomes ◽  
O. S. Smith
Keyword(s):  
Zea Mays ◽  
Embryogenic Callus ◽  
Zea Mays L ◽  
Parental Genotype

Genetic variants in progeny of regenerated maize plants

Genome ◽  
1987 ◽  
Vol 29 (6) ◽  
pp. 834-838 ◽  
Author(s):  
Michael Lee ◽  
R. L. Phillips
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Zea Mays L ◽  
Single Gene ◽  
Callus Cultures ◽  
Regenerated Plants ◽  
Two Generations ◽  
Frequent Variant ◽  
Maize Plants ◽  
Regenerated Plant

Tissue culture has been shown to be a method of generating genetic variation in regenerated plants and their progeny for several maize (Zea mays L.) genotypes. The objectives of this study were to (i) estimate the frequency and types of variants arising from maize tissue cultures, (ii) investigate the effect of culture age on the frequency of variants per regenerated plant, and (iii) estimate the frequency of sectoring among regenerated plants of an F3 from Oh43/A188 genetic background that had not been examined previously for genetic stability in culture. Organogenic callus cultures were initiated from immature F3 embryos for several Oh43ms isoline × A188 crosses. Plants were regenerated either 3 to 4 or 8 to 9 months after culture initiation. Progenies of 248 plants regenerated from 74 cultures were scored for kernel, seedling, and other sporophytic variants following one or two generations of self-pollination. The frequency of variants per regenerated plants increased from 0.5 after 3 to 4 months of culture to 1.3 after 8 to 9 months. A total of 44 variant phenotypes were observed. Defective kernels were the most frequent variant. Most variants were inherited as single-gene recessives. Segregation patterns suggested that the ear and tassel of several (40 of 80) self-pollinated, regenerated plants were genetically discordant. Key words: Zea mays L., tissue culture, somaclonal variation, chimera, qualitative variation.

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