Electron microscopy of neurosecretory nerve fibres in the neural lobe of the embryonic mouse

1974 ◽  
Vol 149 (3) ◽  
Author(s):  
Laila Eurenius ◽  
Rune Jarsk�r
1967 ◽  
Vol 2 (3) ◽  
pp. 349-358
Author(s):  
R. M. EAKIN ◽  
JANE A. WESTFALL ◽  
M. J. DENNIS

The eye of a nudibranch, Hermissenda crassicornis, was studied by light and electron microscopy. Three kinds of cells were observed: large sensory cells, each bearing at one end an array of microvilli (rhabdomere) and at the other end an axon which leaves the eye by the optic nerve; large pigmented supporting cells; and small epithelial cells, mostly corneal. There are five sensory cells, and the same number of nerve fibres in the optic nerve. The receptor cells contain an abundance of small vesicles, 600-800 Å in diameter. The lens is a spheroidal mass of osmiophilic, finely granular material. A basal lamina and a capsule of connective tissue enclose the eye. In some animals the eye is ‘infected’ with very small bodies, 4-5 µ in diameter, thought to be symbionts.


1966 ◽  
Vol 11 (8) ◽  
pp. 845-IN17 ◽  
Author(s):  
S. Miyoshi ◽  
S. Nishijima ◽  
I. Imanishi

1972 ◽  
Vol 11 (3) ◽  
pp. 875-886
Author(s):  
M. BERLINROOD ◽  
S. M. McGEE-RUSSELL ◽  
R. D. ALLEN

Tissue-cultured nerve fibres derived from 3 species of amphibian embryos were composed of bundles of neurites, when viewed by electron microscopy, and contained particles moving in both directions by saltations. Photokymographic analysis of ciné microscopy records taken with Zeiss differential interference optics demonstrated instantaneous particle velocities ranging from 0.15 to 1.8 µm 8-1 (13-155 mm/day) in Rana pipiens cultures; 0.16-0.8 µm s-1 (14-74 mm/day) in Ambystoma tigrinum cultures and 0.6-2.25 µm s-1 (45-194 mm/day) in Xenopus laevis cultures. Measured pathlengths for particles saltations ranged from 4 to 63 µm. No correlations between particle velocity and pathlength were found.


1977 ◽  
Vol 73 (2) ◽  
pp. 197-205 ◽  
Author(s):  
E. L. RODRÍGUEZ ◽  
ECHANDÍA, J. C. CAVICHIA ◽  
E. M. RODRÍGUEZ

SUMMARY The effects of an injection of vinblastine (Vbl) into the median eminence on the structure, fine structure and antidiuretic hormone (ADH) content of the hypothalamo-neurohypophysial system in the rat is reported. The animals were studied on days 3, 8 and 25 after the injection of 1 or 5 mm-Vbl (3 μl). Significant changes were observed only in the 5 mm-Vbl-injected animals. Their median eminence extracts showed a progressive accumulation of ADH whereas ADH depletion occurred in the neural lobe extracts. On day 8 after injection, the animals exhibited strong polidipsia although considerable amounts of ADH still remained within the neural lobe. The ADH content of the plasma samples was consistently below the sensitivity of the method (5 μu.). The light microscopic analysis showed accumulation of Gomori-stainable products in the median eminence and a striking depletion of this material from the neural lobe. Electron microscopy revealed accumulation of neurosecretory vesicles and other inclusions proximal to the site of injection in the median eminence together with some evidence of nerve fibre degeneration. Few neurosecretory terminals were found in the neural lobe of the 8-day experimental rats. They had been engulfed by pituicytes for digestion. Recuperation of the normal ADH content of both median eminence and neural lobe was found to occur on day 25 after the Vbl injection. Simultaneously, the neural lobe refilled with Gomori-positive materials and neurosecretory terminals reappeared. The results suggest (1) reversible blockade of axoplasmic transport at the site of the Vbl injection; (2) reversible degeneration of neurosecretory terminals and (3) reversible blockade of ADH release in the surviving terminals of the neural lobe.


1971 ◽  
Vol 119 (4) ◽  
pp. 534-551 ◽  
Author(s):  
Peter S. Spencer ◽  
A. R. Lieberman

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