Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh

RAPD markers have been used successfully in genetic analysis of several crop plants. This method poses difficulties with a highly heterozygous species such as Theobroma cacao because of the dominant phenotypic expression of bands. A backcross family derived from ctultivars Catongo and Pound 12 was analyzed to determine the efficacy of RAPD markers in analyzing cacao populations. A preliminary screen of the parents and the F1 plant used as the backcross parent was conducted with 180 RAPD primers; of these, 26% were polymorphic and reproducible and produced 104 storable loci. Genomic DNA from 54 individuals of the backcross population was then amplified with these primers; 68.3 % of the loci segregated as expected in a Mendelian fashion. Separation of RAPD fragments on acrylamide revealed an additional polymorphic locus from one primer that was indistinguishable on agarose. The results demonstrated that RAPD markers can be used to study the cacao genome.

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Genetic analysis of honey bee, Apis dorsata populations using random amplified polymorphic DNA (RAPD) markers

Journal of King Saud University - Science ◽

10.1016/j.jksus.2020.10.015 ◽

2021 ◽

Vol 33 (1) ◽

pp. 101218

Author(s):

Samina Qamer ◽

Amal Abdullah Al-Abbadi ◽

Muhmmad Sajid ◽

Farkhanda Asad ◽

Muhammad Fiaz Khan ◽

...

Keyword(s):

Genetic Analysis ◽

Honey Bee ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Apis Dorsata

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Genetic analysis of homokaryons from a basidiome of Laccaria bicolor using random amplified polymorphic DNA (RAPD) markers

Mycological Research ◽

10.1016/s0953-7562(09)81222-7 ◽

1995 ◽

Vol 99 (11) ◽

pp. 1361-1366 ◽

Cited By ~ 20

Author(s):

R.L. Doudrick ◽

V.L. Raffle ◽

C.D. Nelson ◽

G.R. Furnier

Keyword(s):

Genetic Analysis ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Laccaria Bicolor

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EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

FLORA AND FAUNA ◽

10.33451/florafauna.v23i2pp461-467 ◽

2017 ◽

Vol 23 (2) ◽

Cited By ~ 1

Author(s):

SUNITA BORDE ◽

ASAWARI FARTADE ◽

AMOL THOSAR ◽

RAHUL KHAWAL

Keyword(s):

Fresh Water ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Band Pattern ◽

Genomic Variation ◽

Polymorphic Band ◽

Rapd Profile ◽

Band Size ◽

Pcr Product ◽

The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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