Genetic analysis of stunted growth by nuclear-cytoplasmic interaction in interspecific hybrids of Capsicum by using RAPD markers

Low-density randomly amplified polymorphic DNA (RAPD) markers of sweetpotato [Ipomoea batatus (L.) Lam.; 2n = 6x = 90] were constructed from 76 pseudotestcross progenies obtained from `Vardaman' × `Regal'. Of 460 primers, 84 generating 196 well-resolved repeatable markers were selected for genetic analysis. `Vardaman' and `Regal' testcross progenies were analyzed for segregation and linkages of RAPD markers. Type of polyploidy, autopolyploidy, or allopolyploidy is uncertain in sweetpotato and was examined in this study using the ratio of nonsimplex to simplex RAPD markers and the ratio of simplex RAPD marker pairs linked in repulsion to coupling. Both measures indicated autopolyploidy. Low-density RAPD linkage maps of `Vardaman' and `Regal' were constructed from simplex RAPD marker linkage analysis. Duplex and triplex markers were then mapped manually into the simplex marker map. Homologous linkage groups were identified using nonsimplex RAPD markers and three homologous groups were found in each of the parent maps. Use of nonsimplex markers increased mapping efficiency. The `Vardaman' map had a predicted coverage of 10.5% at a 25-cM interval of the genome size of 5024 cM. In `Regal', genome coverage was estimated to be 5.6% at a 25-cM interval of the genome size of 6560 cM. Therefore, average chromosome length was ≈56 to 73 cM.

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Inheritance of Random Amplified Polymorphic DNA (RAPD) Markers in Theobroma cacao L.

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.120.4.681 ◽

1995 ◽

Vol 120 (4) ◽

pp. 681-686 ◽

Cited By ~ 9

Author(s):

C.M. Ronning ◽

R.J. Schnell ◽

D.N. Kuhn

Keyword(s):

Genetic Analysis ◽

Theobroma Cacao ◽

Genomic Dna ◽

Rapd Markers ◽

Polymorphic Locus ◽

Random Amplified Polymorphic Dna ◽

Phenotypic Expression ◽

Backcross Population ◽

Backcross Family ◽

Theobroma Cacao L

RAPD markers have been used successfully in genetic analysis of several crop plants. This method poses difficulties with a highly heterozygous species such as Theobroma cacao because of the dominant phenotypic expression of bands. A backcross family derived from ctultivars Catongo and Pound 12 was analyzed to determine the efficacy of RAPD markers in analyzing cacao populations. A preliminary screen of the parents and the F1 plant used as the backcross parent was conducted with 180 RAPD primers; of these, 26% were polymorphic and reproducible and produced 104 storable loci. Genomic DNA from 54 individuals of the backcross population was then amplified with these primers; 68.3 % of the loci segregated as expected in a Mendelian fashion. Separation of RAPD fragments on acrylamide revealed an additional polymorphic locus from one primer that was indistinguishable on agarose. The results demonstrated that RAPD markers can be used to study the cacao genome.

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Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132010000200017 ◽

2010 ◽

Vol 53 (2) ◽

pp. 375-387 ◽

Cited By ~ 8

Author(s):

Luciana do Valle Rego Oliveira ◽

Ricardo Tadeu de Faria ◽

Claudete de Fátima Ruas ◽

Paulo Maurício Ruas ◽

Melissa de Oliveira Santos ◽

...

Keyword(s):

Molecular Markers ◽

Genetic Variability ◽

Genetic Analysis ◽

Genetic Relationship ◽

Genetic Similarity ◽

Core Collection ◽

Rapd Markers ◽

Dna Amplification ◽

Breeding Programs

In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.

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