HUBUNGAN KEKERABATAN FENETIK 7 SPESIES JERUK DI DATARAN TINGGI BENER MERIAH

The study about “Phenetic Relationship of 7 Species of Orange Plants in BenerMeriah Highlands” was inducted back in August until September 2017. The aim of this study is to describe the morphological characterization of 7 species of orange plants in BenerMeriah highlands and to understand the phenetic relationship of 7 species of oranges plant in BenerMeriah highlands. The method used in this study was observation with the type of mixture between qualitative and quantitative. The subjects of this study are 7 species of orange plants, namely: a) Citrus reticulata; b) Citrus amblycarpa; c) Citrus hystrix; d) Citrus nobilis; e) Citrus maxima; f) Citrus sinensis; and g) Citrus aurantifolia. The parameters of this study were the morphological characteristic of stems, leaves, fruits and flowers. The collected data were analyzed descriptively, where the similarities were measured using Similarity Index (SI) and the dissimilarities were measured using Dissimilarity Index (DI) to classify the total of 7 species of orange plants using cluster analysis. According to the morphological characteristics, it could be concluded that there are several differences and similarities among the 7 species of orange plants found in BenerMeriah Regency. The combination of Citrus hystrix with Citrus aurantifolia have the closest phenetic relationship, which have 66 of SI. The other combinations that also have close phenetic relationship are Citrus hystrix with Citrus maxima and Citrus nobilis with Citrus sinensis. The pheneticrelationship that have the highest DI is the combination of Citrus amblycarpa with Citrus maxima, which have 88 of DI

Analysis of morphological characteristics and phenetic relationship of ebony (Diospyros spp.) in Indonesia

Rindyaastuti R, Hapsati L, Wibowo AT. 2021. Analysis of morphological characteristics and phenetic relationship of ebony (Diospyros spp.) in Indonesia. Biodiversitas 22: 2738-2753. Diospyros L. (Ebenaceae) is an economically important genus that covers over 500 plant species. Members of this group are known to produce hard, dark, high-quality timbers known as ebony trees, while other members are known as persimmon trees. There is limited information on the morphological characters and phenetic relationship of this genus from Indonesian archipelago. In this work, we reported the phenetic clustering of 14 species of Indonesian Diospyros, based on plant habit, stem, leaves, and fruit characters, which was analysed using UPGMA and Jaccard similarity index. The phenetic dendrogram divided the 14 species of Diospyros into two main clusters which separating Subgen. Maba and Eudiospyros. Further, subgen. Maba was clustered into a single cluster while subgen. Eudiospyros was divided into 3 sub-clusters as sister groups. The clustering in Eudiospyros was supported by low SI and bootstrap value, demonstrating the high morphological variation of the subgenus. The sectional separation of Diospyros spp. was considered as paraphyletic. In general, our phenetic clustering exhibited suitability and relevancy with previous classification. Fruit size and trichomes are very important in this phenetic clustering, especially to form Eudiospyros subgenus. Fruit trichomes were also special characters related to biogeographical distribution following the Wallace line theory.

Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java

Lysurus is one of the unique genera in Basidiomycetes. It has a stinky odor and slime on the head. The unusual-shaped makes the species in Lysurus easily to be identified. One of Lysurus had been found in West Java, Indonesia namely L. periphragmoides. The specimen was deposited into Herbarium Bogoriense with code BO 24418. This study aimed to obtain specimens and characterize the Lysurus BO 24418 using morphological characteristics, and analyze the phenetic relationship among Lysurus species. Lysurus BO 24418 has two phases (egg and mushroom). The egg phase is usually hypogeous underground. The mushroom has a head and stem. The head bears the mature spore with slime distribute malodor. The stem has a hollow and spongy texture with a yellowish color. The numerical data of morphological characters of species in Lysurus were analyzed using NTSys ver 2.1 software. Ten characters were used to build a dendrogram using Sequential Agglomerative Hierarchical Nested (SAHN) cluster analysis with Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Phallus indusiatus was selected as an outgroup. The analyses showed the specimen was classified as L. periphragmoides with 100% of similarity coefficient and it was close L. gardneri with 40,4% of similarity coefficient. The characters that cluster among them are stem surface, head type, egg diameter, and spore max length

Morpho-Molecular Study on some Taxa of Apocynaceae Sensu Lato

Apocynaceae sensu lato (s.l.) is treated as distinct family in some taxonomic systems while in others is treated as two separated families viz. Apocynaceae sensu stricto (s.s.) and Asclepiadaceae. So the main objective of the present study was to adopt one of the two views. The morphological characters viz. whole plant, lamina vein architecture and lamina epidermal characters of 20 wild and ornamental species were examined using LM and SEM. The data were numerically analyzed to detect the phenetic relationship among the studied species. DNA barcoding based on the sequencing of rbcL gene was cladistically analyzed to detect the phylogenetic relationship among the studied species. The generated phenogram showed a clear separation of two subseries; one of them belonged to Apocynaceae and the other with the remaining taxa belonging to Asclepiadaceae. The obtained cladogram showed that all of the studied species were divided into four lineages. It is concluded that the phenetic analysis supports the treatment of Apocynaceae s.l. as two distinct families; Apocynaceae s.s. Asclepiadaceae contrary of the phylogenetic one that supports the treatment of Apocynaceae s.s. and Asclepiadaceae as one family (Apocynaceae s.l.) with four subfamilies viz. Apocynoideae, Rauvolfiodeae, Asclepiadoideae and Periplocoideae. © 2021 Friends Science Publishers

VARIASI DAN HUBUNGAN FENETIK AKSESI KUNYIT DI YOGYAKARTA DAN SEKITARNYA

Tujuan penelitian ini adalah menentukan variasi morfologis dan anatomis tiap aksesi Curcuma longa, serta hubungan fenetik pada spesies antar aksesi Curcuma longa. Sebanyak 13 sampel diambil di area Yogyakarta dan sekitarnya, karakter morfologis yang dibandingkan bersumber dari akar, rimpang, daun meliputi bentuk dan ukuran. Karakter anatomis diamati pada daun dan rimpang. Penentuan karakter morfologis berdasarkan Protection of Plant Varieties dan Farmers’ Rights Authority. Hubungan fenetik menggunakan perbandingan karakter morfologis dan anatomis spesies Curcuma longa yang diteliti. Hasil penelitian menunjukkan variasi morfologis Curcuma longa di Yogyakarta dan sekitarnya memiliki perbedaan tinggi tanaman, morfologis daun, serta morfologis akar dan rimpang. Variasi karakter anatomis Curcuma longa memiliki perbedaan ketebalan epidermis daun, ukuran dan densitas stomata, ketebalan berkas pengangkut, ketebalan epidermis dan hipodermis rimpang. Berdasarkan analisis fenetik Curcuma longa di Yogyakarta dan sekitarnya dapat diketahui bahwa hubungan kekerabatan fenetik berdasarkan karakter morfologis terbagi menjadi dua klaster besar dan empat subklaster, sedangkan berdasarkan karakter anatomis terbagi menjadi dua klaster dan enam subklaster.VARIATION AND PHENETIC RELATIONSHIP OF TUMERIC ACCESSIONS IN YOGYAKARTA AND SURROUNDING AREASThe purpose of this study was to determine the morphological and anatomical variations of each accession of Curcuma longa, as well as the phenetic relationship among accessions of Curcuma longa. A total of 13 samples were taken in the Yogyakarta and surrounding areas, the morphological characters compared from roots, rhizomes, leaves include shape and size. The anatomical characters were observed in the leaves and rhizomes. The determination of morphological characters based on Protection of Plant Varieties and Farmers’ Rights Authority. The phenetic relationship used a comparison of the morphological and anatomical characters of the Curcuma longa species studied. The results showed that the morphological variations of Curcuma longa in Yogyakarta and its surroundings had differences in plant height, leaf morphology, and root and rhizome morphology. Variations in anatomical characters of Curcuma longa have differences in leaf epidermis thickness, stomata size and density, carrier bundle thickness, epidermis and rhizome hypodermis thickness. Based on the phenetic analysis of Curcuma longa in Yogyakarta and its surroundings, it shows phenetic relationship based on morphological characters is divided into two large clusters and four sub-clusters, while based on anatomical characters it is divided into two clusters and six sub-clusters.

Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers

Sabdanawaty FP, Purnomo, Daryono BS. 2021. Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers. Biodiversitas 22: 193-198. Api-api (Avicennia spp.) from mangrove groups is rich in economic and ecological benefits. Research on the potential of api-api has been extensively done, but no research is specifically focused on the identification of api-api. This study aims to identify species diversity and phenetic relationships of api-api in Java based on morphological and molecular characters. The morphological analysis was based on 35 characters and referred to the descriptor. The PCA analysis on the morphological characters of Avicennia spp. showed a high diversity. Morphological characters have a high effect on grouping patterns such as habitat, leaf shape, leaf tip, leaf base, and petal color. Molecular observations were done on the polymorphism of DNA bands. The ISSR primers used were ISSR02, ISSR04, and ISSR10. PCR amplification of DNA was separated and visualized using a doc gel electrophoresis. The results showed a high diversity based on the percentage of DNA polymorphism. The research resulted in three species of Avicennia spp. in Java, namely Avicennia officinalis, A. alba, and A. marina. Phenetic relationships between Avicennia spp. form was based on similarity in character, not based on the area of origin. The environmental conditions in this study slightly opposed the character of Avicennia spp., which presumably due to the similarity in various marine areas of Java such as temperature, pH, and the required salinity.

The Phenetic Relationship of Ferns (Polypodiaceae) at the Ascent of Cemoro Kandang, Mount Lawu

The fern is one of the highly diverse vascular plant species, which has a unique characteristic. The closely related at the family level because the genera within each family have some uniformity so that the relationship and groupings cannot be distinguished. One example of a diverse fern family is Polypodiaceae. Consequently, it is necessary to simplify the classification; one of them is through phenetic relationship analysis. This study aimed to analyze the phenetic relationship of the Polypodiaceae ferns in Climbing Cemoro, Mouth Lawu enclosure, Karangayar Regency, based on the similarity index on the dendogram. The data were collected using the exploration method and the sporophyte morphological characters were observed. The observations obtained 13 variations of characters. The relationship analysis was carried out with the hierarchical cluster program using SPSS 23. There were six species found from the Polypodiaceae family, there were including Belvisia mucronata, Goniophlebium sp, Lepisorus sp, Pyrrosia piloselloides, Crypsinus taeniatus and Drynaria sparsisora. The finding suggests that six species are divided into two clusters, the Group I consisting of Goniophlebium sp, Lepisorus sp, Lepisorus sp, and Pyrrosia pilloselloides with a similarity coefficient index of 53% and Group II consisting of Drynaria sparsisora, Goniophlebium sp, and Pyrrosia piloselloides with a similarity index of 46%.

The Diversity And Classification Of Intraspecies Of Gembolo (Dioscorea Bulbifera L.) Based On Morphological Character

Gembolo (Dioscorea bulbifera L.) is a dioecious, annual, herbaceous, climbing plants and has heart-shaped leaves. Gembolo has aerial tubers (bulbil) and main tuber that has irregular shapes, as well as many rough roots at the base of the stem. The purpose of this research is to know the phenetic relationship of the germplasm of Gembolo in Special Region of Yogyakarta based on morphological character. The results of this study are expected to provide information on intraspecies diversity, phenetic relationship and Gembolo distribution. Gembolo plant samples were taken from D.I Yogyakarta. The sample was observed based on the difference of morphological character, so the number of accessions obtained from D.I Yogyakarta could be determined. Morphological characters would be described and characterized to determine the Operational Taxonomic Units (OTU’s). Based on morphological character data, then the grouping analysis was done by grouping analysis method and the dendrogram was formed by the method of Unweighted Pair Group Methods using Arithmetic averages (UPGMA) using Multivariate Statistical Program (MVSP) software version 3.1pc. The results showed that gembolo had morphological variation in tuber shape, stem color, leaf shape, leaf base shape and leaf tip shape. Based on the morphological characters, 2 main groups with the value of similarity index of 62,8% was formed. Group I consisted of four accessions with a similarity value of 85%. Group II consisted of eight accessions with a similarity value of 75%. A high similarity based on the morphological character on gembolo accession caused the gembolo plants in Special Region of Yogyakarta did not vary.