Genetic analysis of mutants of Salmonella typhimurium deficient in formate dehydrogenase activity

1973 ◽  
Vol 120 (4) ◽  
pp. 337-340 ◽  
Author(s):  
M. C. Pascal ◽  
F. Casse ◽  
M. Chippaux ◽  
M. Lepelletier

Genetics ◽  
1989 ◽  
Vol 123 (4) ◽  
pp. 625-633 ◽  
Author(s):  
M B Schmid ◽  
N Kapur ◽  
D R Isaacson ◽  
P Lindroos ◽  
C Sharpe

Abstract We have isolated 440 mutants of Salmonella typhimurium that show temperature-sensitive growth on complex medium at 44 degrees. Approximately 16% of the mutations in these strains have been mapped to 17 chromosomal locations; two of these chromosomal locations seem to include several essential genes. Genetic analysis of the mutations suggests that the collection saturates the genes readily mutable to a ts lethal phenotype in S. typhimurium. Physiological characteristics of the ts lethal mutants were tested: 6% of the mutants can grow at high temperature under anaerobic conditions, 17% can grow when the medium includes 0.5 M KCl, and 9% of the mutants die after a 2-hr incubation at the nonpermissive temperature. Most ts lethal mutations in this collection probably affect genes required for growth at all temperatures (not merely during high temperature growth) since Tn10 insertions that cause a temperature-sensitive lethal phenotype are rare.





2006 ◽  
Vol 95 (1) ◽  
pp. 199-203 ◽  
Author(s):  
Marion B. Ansorge-Schumacher ◽  
Sonja Steinsiek ◽  
Werner Eberhard ◽  
Nikolaos Keramidas ◽  
Klaus Erkens ◽  
...  


1987 ◽  
Vol 208 (1-2) ◽  
pp. 279-287 ◽  
Author(s):  
John W. Foster ◽  
Elizabeth A. Holley-Guthrie ◽  
Felicity Warren




Microbiology ◽  
1987 ◽  
Vol 133 (11) ◽  
pp. 3071-3080 ◽  
Author(s):  
H. Fujita ◽  
S. Yamaguchi ◽  
T. Taira ◽  
T. Hirano ◽  
T. Iino


Anaerobe ◽  
1995 ◽  
Vol 1 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Tina S. Haynes ◽  
Dwight J. Klemm ◽  
Joseph J. Ruocco ◽  
Larry L. Barton


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