Heat shock induced phenocopies of dominant mutants of the bithorax complex in Drosophila melanogaster

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

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Ecdysteroid-regulated heat-shock gene expression during Drosophila melanogaster development

Developmental Biology ◽

10.1016/0012-1606(86)90263-0 ◽

1986 ◽

Vol 115 (2) ◽

pp. 434-438 ◽

Cited By ~ 38

Author(s):

Steven R. Thomas ◽

Judith A. Lengyel

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Heat Shock ◽

Heat Shock Gene ◽

Shock Gene ◽

Heat Shock Gene Expression

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Digitonin treatment activates specific genes including the heat-shock genes in salivary glands of Drosophila melanogaster

Developmental Biology ◽

10.1016/0012-1606(88)90440-x ◽

1988 ◽

Vol 130 (1) ◽

pp. 348-355 ◽

Cited By ~ 5

Author(s):

Maroko Myohara ◽

Masukichi Okada

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Salivary Glands ◽

Heat Shock Genes

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Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

Molecular and Cellular Biology ◽

10.1128/mcb.9.1.332 ◽

1989 ◽

Vol 9 (1) ◽

pp. 332-335 ◽

Cited By ~ 7

Author(s):

S E Kelly ◽

I L Cartwright

Keyword(s):

Drosophila Melanogaster ◽

Heat Shock ◽

Heat Shock Protein ◽

Intergenic Region ◽

Small Heat Shock Protein ◽

Dnase I ◽

Developmentally Regulated ◽

Regulatory Interactions ◽

Chromatin Architecture ◽

Heat Shock Protein Genes

Alterations in the pattern of DNase I hypersensitivity were observed on ecdysterone-stimulated transcription of Drosophila melanogaster small heat shock protein genes. Perturbations were induced near hsp27 and hsp22, coupled with an extensive domain of chromatin unfolding in the intergenic region between hsp23 and the developmentally regulated gene 1. These regions represent candidates for ecdysterone regulatory interactions.

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The origin of pattern duplications in segment polarity mutants of Drosophila melanogaster

Development ◽

10.1242/dev.87.1.129 ◽

1985 ◽

Vol 87 (1) ◽

pp. 129-135

Author(s):

Alfonso Martinez-Arias ◽

Philip W. Ingham

Keyword(s):

Drosophila Melanogaster ◽

Bithorax Complex ◽

Anterior Compartment ◽

Larval Cuticle ◽

Segment Polarity ◽

Reversed Polarity ◽

Segmental Identity

Mutations of the segment polarity group in Drosophila melanogaster produce additional denticles with reversed polarity in every segment of the larval cuticle. We have investigated the effect of mutations in different elements of the bithorax complex on the segmental identity of these additional pattern elements. Our results suggest that they are derived, primarily, from the anterior compartment of each segment.

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The pattern of campaniform sensilla on the wing and haltere of Drosophila melanogaster and several of its homeotic mutants

Development ◽

10.1242/dev.71.1.41 ◽

1982 ◽

Vol 71 (1) ◽

pp. 41-61

Author(s):

Eric S. Cole ◽

John Palka

Keyword(s):

Drosophila Melanogaster ◽

Campaniform Sensilla ◽

Bithorax Complex ◽

Homeotic Transformation ◽

Anterior Compartment

A detailed mapping and description of campaniform sensilla on the wing and haltere of Drosophila melanogaster is provided. Six types of sensilla are distinguished. Similarities in the pattern of their distribution on the dorsal and ventral surfaces of each appendage, as well as between the wing and haltere, are apparent. These data are used to assess the quality of homeotic transformation in several mutants of the bithorax complex in which the halteres are transformed into wings. Flies homozygous for abxbx3pbx produce a complete inventory of wing sensilla on the homeotic appendage. In abx, bx3 and bx3pbx homozygotes the transformation of haltere into wing is incomplete, and each mutant shows characteristic fields of haltere and wing sensilla. It appears that specific regions of the anterior haltere compartment require different combinations of mutant alleles to produce a distinct homeotic transformation. Furthermore, the pbx mutation appears to influence expression of the bx3 mutation within the anterior compartment.

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