Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance

1986 ◽  
Vol 5 (3) ◽  
pp. 212-214 ◽  
Author(s):  
J. P. Helgeson ◽  
G. J. Hunt ◽  
Geraldine T. Haberlach ◽  
Sandra Austin
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Resistance Gene ◽  
Somatic Hybrids ◽  
Leaf Roll ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Solanum Brevidens ◽  
Potato Leaf

Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding

2015 ◽  
Vol 123 (3) ◽  
pp. 579-589 ◽  
Author(s):  
Poonam Chandel ◽  
Jagesh Kumar Tiwari ◽  
Nilofer Ali ◽  
Sapna Devi ◽  
Shashi Sharma ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Somatic Hybrids ◽  
Resistance Breeding ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Potential Sources

scholarly journals Performance of Transgenic Potato Containing the Late Blight Resistance Gene RB

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Dennis A. Halterman ◽  
Lara Colton Kramer ◽  
Susan Wielgus ◽  
Jiming Jiang
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Transgenic Potato ◽  
Field Trials ◽  
Blight Resistance ◽  
Solanum Bulbocastanum ◽  
Wild Potato Species ◽  
Foliar Resistance ◽  
In The Wild

Late blight of potato, caused by Phytophthora infestans, is one of the most devastating diseases of potato. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum through map-based cloning. The full-length gene coding sequence, including the open reading frame and promoter, has been integrated into cultivated potato (S. tuberosum) using Agrobacterium-mediated transformation. RB-containing transgenic plants were challenged with P. infestans under optimal late blight conditions in greenhouse experiments. All transgenic lines containing RB exhibited strong foliar resistance. Field-grown transgenic tubers also were tested for resistance to P. infestans. In contrast to the foliar resistance phenotype, RB-containing tubers did not exhibit increased resistance. Two years of field trials were used to ascertain whether the presence of RB had any effect on tuber yield. We were unable to detect any significant effect on tuber size or yield after addition of the resistance gene to several S. tuberosum cultivars.

Evaluation of the RPi-ber late blight resistance gene for tuber resistance in the field and laboratory

2011 ◽  
Vol 130 (4) ◽  
pp. 464-468 ◽  
Author(s):  
Hilary Mayton ◽  
Gilda Rauscher ◽  
Ivan Simko ◽  
William E. Fry
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Tuber Resistance

scholarly journals Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background

2007 ◽  
Vol 115 (1) ◽  
pp. 101-112 ◽  
Author(s):  
J. Śliwka ◽  
H. Jakuczun ◽  
R. Lebecka ◽  
W. Marczewski ◽  
C. Gebhardt ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Late Blight Resistance ◽  
Wild Relatives ◽  
Blight Resistance ◽  
Cultivated Potato ◽  
Plant Maturity

scholarly journals Higher Copy Numbers of the Potato RB Transgene Correspond to Enhanced Transcript and Late Blight Resistance Levels

2009 ◽  
Vol 22 (4) ◽  
pp. 437-446 ◽  
Author(s):  
James M. Bradeen ◽  
Massimo Iorizzo ◽  
Dimitre S. Mollov ◽  
John Raasch ◽  
Lara Colton Kramer ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Late Blight ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Rna Silencing ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Transcript Levels ◽  
Cultivated Potato ◽  
Copy Numbers

Late blight of potato ranks among the costliest of crop diseases worldwide. Host resistance offers the best means for controlling late blight, but previously deployed single resistance genes have been short-lived in their effectiveness. The foliar blight resistance gene RB, previously cloned from the wild potato Solanum bulbocastanum, has proven effective in greenhouse tests of transgenic cultivated potato. In this study, we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions. In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides. RB copy numbers and transcript levels were estimated with transgene-specific assays. Disease resistance was enhanced as copy numbers and transcript levels increased. The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels. Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested. We conclude that even in these ultra–high copy number lines, innate RNA silencing mechanisms have not been fully activated. Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing. Strategies for the deployment of RB are discussed in light of the current research.

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