A physical map of wheat chloroplast DNA showing the location of the structural genes for the ribosomal RNAs and the large subunit of ribulose 1,5-bisphosphate carboxylase

1981 ◽  
Vol 183 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Catherine M. Bowman ◽  
Barbara Koller ◽  
Hajo Delius ◽  
Tristan A. Dyer
Keyword(s):  
Chloroplast Dna ◽  
Physical Map ◽  
Large Subunit ◽  
Structural Genes ◽  
Ribosomal Rnas ◽  
Bisphosphate Carboxylase

Daffodil Chromoplast DNA: Comparison with Chloroplast DNA, Physical Map, and Gene Localization

1987 ◽  
Vol 42 (1-2) ◽  
pp. 118-122 ◽  
Author(s):  
Paul Hansmann
Keyword(s):  
Chloroplast Dna ◽  
Physical Map ◽  
Large Subunit ◽  
Plastid Dna ◽  
Cytochrome F ◽  
Restriction Endonucleases ◽  
Gene Localization ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Ribulose Bisphosphate Carboxylase Oxygenase

Abstract In daffodil, development of chloroplasts or chromoplasts is not accompanied by plastid DNA modification. This has been shown by comparing the fragment pattern of different restriction endonucleases, and by the lack of methylation of CCGG sequences. A physical map has been constructed for the plastome using the restriction endonucleases Sal I, Pst I, and Bgl I. The fragments containing the genes for the large subunit of ribulose bisphosphate carboxylase/oxygenase (rbcL), the alpha, beta, and epsilon subunits of the ATP synthase complex (atpA , atpB, atpE ), cytochrome f(petA ), and for the 51 kDa chlorophyll apoprotein of photosystem II (psbB) have been identified. The respective gene locations correspond to those on spinach chloroplast DNA.

scholarly journals LINKAGE OF A KNOWN CHLOROPLAST GENE MUTATION TO THE UNIPARENTAL GENOME OF CHLAMYDOMONAS REINHARDII

Genetics ◽  
1983 ◽  
Vol 105 (3) ◽  
pp. 559-579
Author(s):  
Laurens J Mets ◽  
Lois J Geist
Keyword(s):  
Chloroplast Dna ◽  
Physical Map ◽  
Large Subunit ◽  
Physical Structure ◽  
Ribulose Bisphosphate Carboxylase ◽  
Equal Opportunities ◽  
Ribulose Bisphosphate ◽  
Bisphosphate Carboxylase ◽  
Chlamydomonas Reinhardii ◽  
Gene Map

ABSTRACT Data are presented that associate three new markers with the uniparental linkage group in Chlamydomonas reinhardii. One of these, mutant 10-6C, is a genetic marker for the structural gene of the large subunit of ribulose bisphosphate carboxylase. These results provide the first direct link between the uniparental gene map and the physical map of chloroplast DNA. The other two markers, Dr2 (DCMU resistant) and 8-36C (deficient in photosystem II activity), map to a single locus. The data suggest that mixing in zygotic chloroplasts may not be complete so that input genomes do not have equal opportunities to recombine. The data are not compatible with simple linear or circular maps but can be explained on the basis of the known physical structure of chloroplast DNA.

THE NATURE OF NUCLEOTIDE SEQUENCE DIVERGENCE BETWEEN BARLEY AND MAIZE CHLOROPLAST DNA

Genetics ◽  
1984 ◽  
Vol 106 (4) ◽  
pp. 735-749
Author(s):  
Gerard Zurawski ◽  
Michael T Clegg ◽  
Anthony H D Brown
Keyword(s):  
Chloroplast Dna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Leader Region ◽  
Coding Region ◽  
Nucleotide Substitutions ◽  
Bisphosphate Carboxylase ◽  
Noncoding Regions ◽  
The Third ◽  
Maize Chloroplast

ABSTRACT Analysis of a 2175-base pair (bp) SmaI-HindIII fragment of barley chloroplast DNA revealed that rbcL (the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase) and atpB (the gene for the β subunit of ATPase) are transcribed divergently and are separated by an untranscribed region of 155-166 bp. The rbcL mRNA has a 320-residue untranslated leader region, whereas the atpB mRNA has a 296- to 309-residue leader region. The sequence of these regions, together with the initial 113 bp of the atpB-coding region and the initial 1279 bp of the rbcL-coding region, is compared with the analogous maize chloroplast DNA sequences. Two classes of nucleotide differences are present, substitutions and insertions/deletions. Nucleotide substitutions show a 1.9-fold bias toward transitions in the rbcL-coding region and a 1.5-fold bias toward transitions in the noncoding region. The level of nucleotide substitutions between the barley and maize sequences is about 0.065/bp. Seventy-one percent of the substitutions in the rbcL-coding region are at the third codon position, and 95% of these are synonymous changes. Insertion/deletion events, which are confined to the noncoding regions, are not randomly distributed in these regions and are often associated with short repeated sequences. The extent of change for the noncoding regions (about 0.093 events/bp) is less than the extent of change at the third codon positions in the rbcL-coding region (about 0.135 events/bp), including insertion/delection events. Limited sequence analysis of the analogous DNA from a wild line (Hordeum spontaneum) and a primitive Iranian barley (H. vulgare) suggested a low rate of chloroplast DNA evolution. Compared to spinach chloroplast DNA, the barley rbcL-atpB untranslated region is extremely diverged, with only the putative rbcL promoters and ribosome-binding site being extensively conserved.

Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA

Cell ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 1007-1014 ◽  
Author(s):  
David M. Lonsdale ◽  
Tony P. Hodge ◽  
Christopher J. Howe ◽  
David B. Stem
Keyword(s):  
Mitochondrial Dna ◽  
Chloroplast Dna ◽  
Large Subunit ◽  
Subunit Gene ◽  
Bisphosphate Carboxylase

scholarly journals Chloroplast Genome Evolution in the Genus Avena

Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 613-621
Author(s):  
Koji Murai ◽  
Koichiro Tsunewaki
Keyword(s):  
Chloroplast Genome ◽  
Restriction Site ◽  
Physical Map ◽  
Fragment Size ◽  
Large Subunit ◽  
Single Copy ◽  
Rbcl Gene ◽  
Type I ◽  
Bisphosphate Carboxylase ◽  
Chloroplast Genomes

ABSTRACT The genus Avena contains five different chloroplast genomes, I-V. A physical map of chloroplast (ct) DNA of Avena sativa (type I chloroplast genome) was constructed using three restriction endonucleases, PstI, SalI and SmaI. This genome is ca. 135.5 kbp in size, and contains two inverted repeats of ca. 22.5 kbp each, separated by a large (ca. 79.0 kbp) and small (ca. 12.5 kbp) single copy region. The rbcL gene which codes for the large subunit of ribulose 1,5-bisphosphate carboxylase, was located in the map. Restriction fragment patterns of all five chloroplast genomes were compared, and among them five fragment size and five restriction site mutations were disclosed. Four site mutations were found in two or more chloroplast genomes, the other site and five fragment size mutations were specific to one or another of the chloroplast genomes. A dendrogram showing phylogenetic relationships among the five chloroplast genomes, based on the distribution of the common and specific mutations among them, indicates that chloroplast genome divergence characterized by three restriction site mutations occurred first between two diploid groups, each carrying A and C genome (nuclear), respectively, followed by further speciation in each group.

scholarly journals The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA

1981 ◽  
Vol 9 (14) ◽  
pp. 3251-3270 ◽  
Author(s):  
Gerard Zurawski ◽  
Brigitte Perrot ◽  
Warwick Bottomley ◽  
Paul R. Whitfeld
Keyword(s):  
Chloroplast Dna ◽  
Large Subunit ◽  
Spinach Chloroplast ◽  
Bisphosphate Carboxylase
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