Karyotype analysis of Meloidogyne hapla by 3-D reconstruction of synaptonemal complexes from electron microscopy of serial sections

Chromosoma ◽  
1978 ◽  
Vol 70 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Paul Goldstein ◽  
A. C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Karyotype Analysis ◽  
Meloidogyne Hapla ◽  
Serial Sections ◽  
Synaptonemal Complexes

Karyotype analysis of the plant-parasitic nematode Heterodera glycines by electron microscopy. 1. The diploid

1979 ◽  
Vol 40 (1) ◽  
pp. 171-179
Author(s):  
P. Goldstein ◽  
A.C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Parasitic Nematode ◽  
Heterodera Glycines ◽  
Karyotype Analysis ◽  
Serial Sections ◽  
Synaptonemal Complexes ◽  
3 Dimensional ◽  
Recombination Nodules ◽  
Dimensional Reconstruction ◽  
Plant Parasitic

Heterodera glycines is a diploid amphimictic nematode with n = 9 chromosomes. Nine normal synaptonemal complexes (SC) were detected following 3-dimensional reconstruction of pachytene nuclei from electron microscopy of serial sections. Regions of unique ‘modified synaptonemal complexes’ (MSC) were observed along 2 SCs. These consist of a heterochromatic knob within which the SC appears either disorganized or stacked in layers of lateral elements. Its function is not known. Recombination nodules and ‘cylindrical granular complexes’, were not observed in H. glycines.

Pachytene karyotype analysis of tetraploid Meloidogyne hapla females by electron microscopy

Chromosoma ◽  
1981 ◽  
Vol 84 (3) ◽  
pp. 405-412 ◽  
Author(s):  
Paul Goldstein ◽  
A. C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Karyotype Analysis ◽  
Meloidogyne Hapla ◽  
Pachytene Karyotype

scholarly journals Anatomical basis for cell transplantation into mouse seminiferous tubules

Reproduction ◽  
2012 ◽  
Vol 144 (3) ◽  
pp. 385-392 ◽  
Author(s):  
Unai Silván ◽  
Juan Aréchaga
Keyword(s):  
Electron Microscopy ◽  
Cell Transplantation ◽  
Soft Tissues ◽  
Partial Information ◽  
Three Dimensional ◽  
Seminiferous Tubules ◽  
Serial Sections ◽  
Pathological Conditions ◽  
Anatomical Basis ◽  
Scanning Electron

Cell transplantation into the seminiferous tubules is a useful technique for the study of physiological and pathological conditions affecting the testis. However, the precise three-dimensional organization and, particularly, the complex connectivity of the seminiferous network have not yet been thoroughly characterized. To date, the technical approaches to address these issues have included manual dissection under the stereomicroscope, reconstruction of histological serial sections, and injection of contrast dyes, but all of them have yielded only partial information. Here, using an approach based on the microinjection of a self-polymerizing resin followed by chemical digestion of the surrounding soft tissues, we reveal fine details of the seminiferous tubule scaffold and its connections. These replicas of the testis seminiferous network were studied by scanning electron microscopy. The present results not only establish a morphological basis for more precise microinjection into the mouse seminiferous tubules but also enable a more profound investigation of physiological and embryological features of the testis.

Ultrastructural karyology of Spongospora subterranea (Plasmodiophoromycetes)

1992 ◽  
Vol 70 (6) ◽  
pp. 1228-1233 ◽  
Author(s):  
James P. Braselton
Keyword(s):  
Electron Microscopy ◽  
Single Unit ◽  
Spongospora Subterranea ◽  
Unit Membrane ◽  
Potato Tubers ◽  
Haploid Chromosome Number ◽  
Synaptonemal Complexes ◽  
Transmission Electron ◽  
Host Parasite ◽  
Section Analysis

Sporogenic (cystogenous) stages of development of Spongospora subterranea (Wallroth) Lagerheim f.sp. subterranea Tomlinson infecting potato tubers were examined with transmission electron microscopy. Volume of nuclei in transitional Plasmodia was 28.2 ± 8.3 μm3. Serial section analysis revealed 37 synaptonemal complexes, hence the haploid chromosome number was considered to be 37. Total length of synaptonemal complexes per nucleus was 74.6 ± 1.4 μm, with individual synaptonemal complexes ranging in length from 1.34 ± 0.07 μm to 3.48 ± 0.17 μm. No polycomplexes were observed in transitional nuclei. Electron-opaque thickenings of lateral elements occurred irregularly. Additional ultrastructural features of sporogenic plasmodia included end-to-end paired centrioles defining the poles of the nuclei and a host–parasite boundary of a single unit membrane. Key words: karyotype, Plasmodiophoromycetes, Spongospora, synaptonemal complex.

A systematic approach to reconstructing microcircuitry by electron microscopy of serial sections

1980 ◽  
Vol 2 (1-3) ◽  
pp. 265-293 ◽  
Author(s):  
John K. Stevens ◽  
Thomas L. Davis ◽  
Neil Friedman ◽  
Peter Sterling
Keyword(s):  
Electron Microscopy ◽  
Systematic Approach ◽  
Serial Sections

Rehydration of freeze substituted brain tissue for preembedding immuno-electron microscopy

2021 ◽  
Author(s):  
Linnaea E Ostroff ◽  
Janeth Perez-Garza ◽  
Emily Parrish ◽  
Zachary Deane
Keyword(s):  
Electron Microscopy ◽  
Full Potential ◽  
Serial Sections ◽  
Volume Reconstruction ◽  
Additional Advantage ◽  
Brain Circuits ◽  
Immuno Electron Microscopy ◽  
Neuronal Processes ◽  
Molecular Information ◽  
Antibody Labeling

Electron microscopy (EM) volume reconstruction is a powerful tool for investigating the fundamental structure of brain circuits, but the full potential of this technique is limited by the difficulty of integrating molecular information. High quality ultrastructural preservation is necessary for EM reconstruction, and intact, highly contrasted cell membranes are essential for following small neuronal processes through serial sections. Unfortunately, the antibody labeling methods used to identify most endogenous molecules result in compromised morphology, especially of membranes. Cryofixation can produce superior morphological preservation and has the additional advantage of allowing indefinite storage of valuable samples. We have developed a method based on cryofixation that allows sensitive immunolabeling of endogenous molecules, preserves excellent ultrastructure, and is compatible with high-contrast staining for serial EM reconstruction.

Karyotypic analysis of Polymyxa graminis (Plasmodiophoromycetes) based on serial sections of synaptonemal complexes

1984 ◽  
Vol 62 (11) ◽  
pp. 2414-2416 ◽  
Author(s):  
James P. Braselton
Keyword(s):  
Polymyxa Betae ◽  
Serial Sections ◽  
Haploid Number ◽  
Karyotypic Analysis ◽  
Thin Sections ◽  
Polymyxa Graminis ◽  
Synaptonemal Complexes

Three pachytene nuclei of Polymyxa graminis Ledingham were reconstructed from serial thin sections. Thirty synaptonemal complexes (SCs) were counted, indicating the identical haploid number (30) that was reported for Polymyxa betae. SCs of P. graminis and P. betae were similar in structure, and nuclear volumes and total lengths of SCs per nucleus were not significantly different for the two species.

Ultrastructure of region of a low safety factor in inhomogeneous giant axon of the cockroach

1976 ◽  
Vol 39 (4) ◽  
pp. 900-908 ◽  
Author(s):  
M. Castel ◽  
M. E. Spira ◽  
I. Parnas ◽  
Y. Yarom
Keyword(s):  
Electron Microscopy ◽  
Glial Cells ◽  
Extracellular Space ◽  
Light And Electron Microscopy ◽  
Giant Axon ◽  
Serial Sections ◽  
Giant Axons ◽  
Chemical Synapses ◽  
Cytoplasmic Processes ◽  
Periaxonal Space

1. The structure of the ventral giant axons of the cockroach at the level of ganglion T3 was studied by means of light and electron microscopy. 2. From serial sections and cobalt injections, the axons diameter was found to range between 40 and 60 mum at the caudal end of ganglion T3; toward the center of T3 they narrow to 20-40 mum, and again expand to 30-45 mum anteriorly in ganglion T3. 3. Each giant axon sends off several branches, 1-15 mum in diameter, into the neuropil. The giant axons and the bases of their branches are enveloped by cytoplasmic processes of glial cells. The periaxonal space is about 100-200 A. 4. Distally the branches are devoid of glial envelopes and the extracellular space between the branches and other axonal profiles is about 200 A. Terminals with presumptive chemical synapses on the giant axon branches were found. Clear vesicles, 300-400 A in diameter, are seen clustered together. The width of the supposedly synaptic gap is about 100 A. 5. In some areas the branches and other axonal profiles form close appositions.

A method for the sequential study of synaptonemal complexes by light and electron microscopy

1981 ◽  
Vol 59 (4) ◽  
pp. 419-421 ◽  
Author(s):  
J. Navarro ◽  
F. Vidal ◽  
M. Guitart ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Sequential Study
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