Pachytene karyotype analysis of tetraploid Meloidogyne hapla females by electron microscopy

Chromosoma ◽  
1981 ◽  
Vol 84 (3) ◽  
pp. 405-412 ◽  
Author(s):  
Paul Goldstein ◽  
A. C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Karyotype Analysis ◽  
Meloidogyne Hapla ◽  
Pachytene Karyotype

Karyotype analysis of Meloidogyne hapla by 3-D reconstruction of synaptonemal complexes from electron microscopy of serial sections

Chromosoma ◽  
1978 ◽  
Vol 70 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Paul Goldstein ◽  
A. C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Karyotype Analysis ◽  
Meloidogyne Hapla ◽  
Serial Sections ◽  
Synaptonemal Complexes

Chromosomes and karyotype analysis of a liver fluke, Opisthorchis viverrini, by scanning electron microscopy

2012 ◽  
Vol 61 (3) ◽  
pp. 504-507 ◽  
Author(s):  
Worasak Kaewkong ◽  
Wej Choochote ◽  
Pipatpong Kanla ◽  
Wanchai Maleewong ◽  
Pewpan M. Intapan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Liver Fluke ◽  
Karyotype Analysis ◽  
Opisthorchis Viverrini ◽  
Scanning Electron

Triplo-X hermaphrodite of Caenorhabditis elegans: pachytene karyotype analysis, synaptonemal complexes, and pairing mechanisms

1984 ◽  
Vol 26 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Paul Goldstein
Keyword(s):  
Caenorhabditis Elegans ◽  
Karyotype Analysis ◽  
Real Difference ◽  
X Chromosomes ◽  
Synaptonemal Complexes ◽  
Chromosomal Complement ◽  
Male Line ◽  
Pachytene Karyotype

Pairing of the three X chromosomes in the triplo-X strain of Caenorhabditis elegans occurs at pachytene in a two-by-two fashion such that one bivalent and one univalent are formed. The XX bivalent pairs synchronously with the autosomes and the univalent X remains in a similar chromatic state as the rest of the chromosomal complement. Normal tripartite synaptonemal complexes (SC) are formed between all bivalents. The univalent X lacks a SC and an axial core is not observed. The condensation of the univalent X in the triplo-X is different than in the male where the univalent X is heterochromatic. This real difference in condensation states of the chromatin may explain the fact that the univalent X is maintained in the male line yet it is easily lost in the triplo-X strain.

Karyotype analysis of the plant-parasitic nematode Heterodera glycines by electron microscopy. 1. The diploid

1979 ◽  
Vol 40 (1) ◽  
pp. 171-179
Author(s):  
P. Goldstein ◽  
A.C. Triantaphyllou
Keyword(s):  
Electron Microscopy ◽  
Parasitic Nematode ◽  
Heterodera Glycines ◽  
Karyotype Analysis ◽  
Serial Sections ◽  
Synaptonemal Complexes ◽  
3 Dimensional ◽  
Recombination Nodules ◽  
Dimensional Reconstruction ◽  
Plant Parasitic

Heterodera glycines is a diploid amphimictic nematode with n = 9 chromosomes. Nine normal synaptonemal complexes (SC) were detected following 3-dimensional reconstruction of pachytene nuclei from electron microscopy of serial sections. Regions of unique ‘modified synaptonemal complexes’ (MSC) were observed along 2 SCs. These consist of a heterochromatic knob within which the SC appears either disorganized or stacked in layers of lateral elements. Its function is not known. Recombination nodules and ‘cylindrical granular complexes’, were not observed in H. glycines.

The synaptonemal complexes of Caenorhabditis elegans: pachytene karyotype analysis of hermaphrodites from the recessive him-5 and him-7 mutants

1986 ◽  
Vol 82 (1) ◽  
pp. 119-127
Author(s):  
P. Goldstein
Keyword(s):  
Caenorhabditis Elegans ◽  
X Chromosome ◽  
High Frequency ◽  
Karyotype Analysis ◽  
Nuclear Morphology ◽  
Group V ◽  
Haploid Chromosome Number ◽  
High Incidence ◽  
Physical Position ◽  
Pachytene Karyotype

The him-5 and him-7 mutants (high incidence of males) of Caenorhabditis elegans both showed increased rates of X chromosome non-disjunction (16% and 3%, respectively) but him-7 also had a high frequency of autosomal non-disjunction (34%). Synaptonemal complex (SC) karyotype analysis revealed a haploid chromosome number of six in each strain. Alterations in him-7 nuclear morphology were observed but there were no aberrations in SC structure that could account for the increased frequency of autosomal non-disjunction. However, the frequency of X-chromosome non-disjunction occurred at predicted rates on the basis of the number of disjunction regulator regions (DRRs) present on the SCs. The observation that the levels of X-chromosome non-disjunction were not influenced by the increase in the frequency of autosomal non-disjunction supports the notion that the X chromosome is subject to separate controls during meiosis. The him-7 mutant is nested within the rad-4 map region on linkage group V, however, SC analysis did not reveal the physical position on the chromosome because of synaptic adjustment.

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

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