Chromosomal proteins of Drosophila melanogaster and an approach for their localisation on polytene chromosomes

Chromosoma ◽  
1977 ◽  
Vol 63 (1) ◽  
pp. 57-78 ◽  
Author(s):  
R. J. Hill ◽  
Fujiko Watt
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosomes ◽  
Chromosomal Proteins

scholarly journals Chromosomal Distribution of Transposable Elements in Drosophila melanogaster Test of the Ectopic Recombination Model for Maintenance of Insertion Site Number

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 197-204
Author(s):  
Christine Hoogland ◽  
Christian Biémont
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Elements ◽  
Dna Content ◽  
Recombination Rate ◽  
Alternative Hypothesis ◽  
Insertion Site ◽  
Polytene Chromosomes ◽  
Negative Relationship ◽  
Site Occupancy ◽  
Site Number

Abstract Data of insertion site localization and site occupancy frequency of P, hobo, I, copia, mdg1, mdg3, 412, 297, and roo transposable elements (TEs) on the polytene chromosomes of Drosophila melanogaster were extracted from the literature. We show that TE insertion site number per chromosomal division was significantly correlated with the amount of DNA. The insertion site number weighted by DNA content was not correlated with recombination rate for all TEs except hobo, for which a positive correlation was detected. No global tendency emerged in the relationship between TE site occupancy frequency, weighted by DNA content, and recombination rate; a strong negative correlation was, however, found for the 3L arm. A possible dominant deleterious effect of chromosomal rearrangements due to recombination between TE insertions is thus not the main factor explaining the dynamics of TEs, since this hypothesis implies a negative relationship between recombination rate and both TE insertion site number and site occupancy frequency. The alternative hypothesis of selection against deleterious effects of insertional mutations is discussed.

Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes

Chromosoma ◽  
1981 ◽  
Vol 82 (2) ◽  
pp. 205-216 ◽  
Author(s):  
F. Scalenghe ◽  
E. Turco ◽  
J. E. Edström ◽  
V. Pirrotta ◽  
M. Melli
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosomes ◽  
Specific Region

Ultrastructure of polytene chromosomes of Drosophila isolated by microdissection

1980 ◽  
Vol 45 (1) ◽  
pp. 15-30
Author(s):  
M.R. Mott ◽  
E.J. Burnett ◽  
R.J. Hill
Keyword(s):  
Electron Microscope ◽  
Polytene Chromosomes ◽  
Ultrastructural Level ◽  
Chromosomal Proteins ◽  
Linear Arrays ◽  
Ribonucleoprotein Particles ◽  
Acid Protein

Drosophila polytene chromosomes prepared by a new micromanipulative procedure, which avoids acid squashing, have been examined at the ultrastructural level in the electron microscope. Puffs at 2B, 68C, 74EF, 75B and 85EF, have been examined in some detail, along with the chromocentre and various interbands. The ultrastructure of these chromosomes, which have never been exposed to acid protein denaturants, compares favourably with that of classical acid-fixed specimens. Ribonucleoprotein particles in puffs are seen to be organized in linear arrays and evidence is adduced for looped transcription units. Particles with characteristic sizes and morphologies are observed near the chromocentre, in puffs and in interbands. In interbands RNP particles and ‘superbead’-like chromatin particles may be distinguished. Drosophila polytene chromosomes isolated by micro-manipulation should prove useful for the localization of native chromosomal proteins at an ultrastructural level.

A cDNA from Drosophila melanogaster encodes a lamin C-like intermediate filament protein

1993 ◽  
Vol 104 (4) ◽  
pp. 1263-1272 ◽  
Author(s):  
C.A. Bossie ◽  
M.M. Sanders
Keyword(s):  
Drosophila Melanogaster ◽  
Intermediate Filament ◽  
Blot Analysis ◽  
Polytene Chromosomes ◽  
Intermediate Filament Protein ◽  
Chromosome 2 ◽  
Cross Hybridization ◽  
Intermediate Filament Proteins ◽  
Nuclear Lamins ◽  
Filament Protein

A novel intermediate filament cDNA, pG-IF, has been isolated from a Drosophila melanogaster embryonic expression library screened with a polyclonal antiserum produced against a 46 kDa cytoskeletal protein isolated from Kc cells. This 46 kDa protein is known to be immunologically related to vertebrate intermediate filament proteins. The screen resulted in the isolation of four different cDNA groups. Of these, one has been identified as the previously characterized Drosophila nuclear lamin cDNA, Dm0, and a second, pG-IF, demonstrates homology to Dm0 by cross hybridization on Southern blots. DNA sequence analysis reveals that pG-IF encodes a newly identified intermediate filament protein in Drosophila. Its nucleotide sequence is highly homologous to nuclear lamins with lower homology to cytoplasmic intermediate filament proteins. pG-IF predicts a protein of 621 amino acids with a predicted molecular mass of 69,855 daltons. In vitro transcription and translation of pG-IF yielded a protein with a SDS-PAGE estimated molecular weight of approximately 70 kDa. It contains sequence principles characteristic of class V intermediate filament proteins. Its near neutral pI (6.83) and the lack of a terminal CaaX motif suggests that it may represent a lamin C subtype in Drosophila. In situ hybridization to polytene chromosomes detects one band of hybridization on the right arm of chromosome 2 at or near 51A. This in conjunction with Southern blot analysis of various genomic digests suggests one or more closely placed genes while Northern blot analysis detects two messages in Kc cells.

scholarly journals Association of a protease with polytene chromosomes of Drosophila melanogaster.

1980 ◽  
Vol 87 (2) ◽  
pp. 415-419 ◽  
Author(s):  
J Cavagnaro ◽  
D A Pierce ◽  
J C Lucchesi ◽  
C B Chae
Keyword(s):  
Acetic Acid ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Protease Inhibitors ◽  
Polytene Chromosomes ◽  
Fixation Procedure ◽  
Apparent Effect ◽  
Chloromethyl Ketone ◽  
Diisopropyl Fluorophosphate ◽  
Uniform Labeling

Incubation of Drosophila salivary glands with radioactive diisopropyl fluorophosphate results in the uniform labeling of polytene chromosomes. Extensive labeling is seen only when chromosome squashes are prepared by a formaldehyde fixation procedure and not by standard acetic acid techniques. The labeling is inhibited in the presence of tosylphenylalanine chloromethyl ketone and phenylmethane sulfonylfluoride but not by tosyllysine chloromethyl ketone, suggesting that a chymotrypsin-like serine protease is associated with the chromosomes. Protease inhibitors show no apparent effect on heat-shock specific puffing.

Interbands behave as decompacted autonomous units in Drosophila melanogaster polytene chromosomes

Genetica ◽  
2007 ◽  
Vol 132 (3) ◽  
pp. 267-279 ◽  
Author(s):  
Valery F. Semeshin ◽  
Sergey A. Demakov ◽  
Victor V. Shloma ◽  
Tatyana Yu. Vatolina ◽  
Andrey A. Gorchakov ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Polytene Chromosomes

scholarly journals PROPERTIES AND EVOLUTIONARY POTENTIAL OF NEWLY INDUCED TANDEM DUPLICATIONS IN DROSOPHILA MELANOGASTER

Genetics ◽  
1982 ◽  
Vol 102 (1) ◽  
pp. 75-89
Author(s):  
Paul A Roberts ◽  
David J Broderick
Keyword(s):  
Drosophila Melanogaster ◽  
Linkage Disequilibrium ◽  
Tandem Duplication ◽  
Polytene Chromosomes ◽  
Primary Source ◽  
Evolutionary Potential ◽  
X Ray ◽  
Fitness Effects ◽  
New Genes ◽  
Tandem Duplications

ABSTRACT Most of some 33 X-ray-induced duplications recovered as Suppressors of Minute loci proved to be direct tandem duplications. When heterozygous, most duplications were crossover suppressors, and duplications of short to moderate size did not reduce the fitness of their bearers. Crossover suppression by tandem duplication may be attributed to intrastrand foldbacks of the type regularly seen in somatic polytene chromosomes. As a consequence, linkage disequilibrium between duplicated elements and normal chromosomes should be more profound than has been supposed. Tandem duplications appear to be predisposed by reason of frequency of generation, crossover suppression and fitness effects to serve as the primary source of new genes.

Absorption spectrum of Feulgen-stained polytene chromosomes of Drosophila melanogaster

1981 ◽  
Vol 68 (2) ◽  
pp. 248-253 ◽  
Author(s):  
Maria Luiza S. Mello ◽  
Shirlei M. Recco
Keyword(s):  
Absorption Spectrum ◽  
Drosophila Melanogaster ◽  
Polytene Chromosomes

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes

1988 ◽  
Vol 8 (5) ◽  
pp. 1877-1886
Author(s):  
B M Benton ◽  
S Berrios ◽  
P A Fisher
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Transcriptional Regulation ◽  
Salivary Gland ◽  
Indirect Immunofluorescence ◽  
Polytene Chromosomes ◽  
Nuclear Interior ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Larval Salivary Gland

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

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