Evaluation of an enzyme-immunoassay for prostatic acid phosphatase utilizing monoclonal antibodies

Abstract We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.

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Enzyme immunoassay of prostatic acid phosphatase after prostatic examination

Urology ◽

10.1016/0090-4295(88)90430-x ◽

1988 ◽

Vol 32 (5) ◽

pp. 469-473 ◽

Cited By ~ 3

Author(s):

Ayda M.F. El-Shirbiny ◽

P. Shridhar ◽

M.S. Al Adnani ◽

L.K. Suresh

Keyword(s):

Acid Phosphatase ◽

Enzyme Immunoassay ◽

Prostatic Acid Phosphatase

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Experience with a new solid phase enzyme immunoassay for prostatic acid phosphatase

World Journal of Urology ◽

10.1007/bf00326959 ◽

1986 ◽

Vol 4 (4) ◽

pp. 189-192

Author(s):

B. J. Schmitz-Dr�ger ◽

G. Baur ◽

Th. Ebert ◽

G. Pfleiderer ◽

R. Ackermann

Keyword(s):

Acid Phosphatase ◽

Enzyme Immunoassay ◽

Solid Phase ◽

Prostatic Acid Phosphatase

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PURIFICATION AND ENZYME IMMUNOASSAY OF TUMOR MARKERS FOR PROSTATE CANCER: PROSTATIC ACID PHOSPHATASE, PROSTATESPECIFIC ANTIGEN AND CREATINE KINASE BB

The Japanese Journal of Urology ◽

10.5980/jpnjurol1928.75.3_404 ◽

1984 ◽

Vol 75 (3) ◽

pp. 404-413 ◽

Cited By ~ 2

Author(s):

Naoyoshi Morishita ◽

Yuzo Minami ◽

Shigeharu Ogawa ◽

Yutaka Saito

Keyword(s):

Prostate Cancer ◽

Creatine Kinase ◽

Acid Phosphatase ◽

Enzyme Immunoassay ◽

Tumor Markers ◽

Prostatic Acid Phosphatase ◽

Creatine Kinase Bb

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A comparative study of the use of monoclonal antibodies using three different immunohistochemical methods: an evaluation of monoclonal and polyclonal antibodies against human prostatic acid phosphatase.

Journal of Histochemistry & Cytochemistry ◽

10.1177/30.3.7037942 ◽

1982 ◽

Vol 30 (3) ◽

pp. 253-260 ◽

Cited By ~ 45

Author(s):

W Y Naritoku ◽

C R Taylor

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Acid Phosphatase ◽

Polyclonal Antibodies ◽

Prostatic Acid Phosphatase ◽

Mouse Serum ◽

Paraffin Sections ◽

Layer System ◽

Significant Difference ◽

Immunohistochemical Methods

The use of immunohistochemical methods has been advocated for the detection and localization of prostatic acid phosphatase in paraffin sections of human prostate. This article explores the possible advantages of utilizing monoclonal antibodies in this method. Monoclonal antibodies, specific for human prostatic acid phosphatase, were integrated into three different immunohistochemical procedures. In the first method, a three-layer peroxidase-antiperoxidase (PAP) system was employed; the monoclonal antibody was followed by rabbit bridge antibody directed against mouse immunoglobulin and mouse PAP complex. The second method was a three-layer system utilizing biotin-labeled horse anti-mouse antibody as "bridge" antiserum between the primary monoclonal antibody and an avidin-biotin-horseradish peroxidase complex. The third method was a four-layer system; the monoclonal antibody was followed by rabbit anti-mouse serum, swine anti-rabbit immunoglobulin as the bridge antibody and rabbit PAP complex. It was found that some, but not all, monoclonal antibodies can be used for the detection of prostatic acid phosphatase in paraffin sections. The four-layer PAP method was found to be the most sensitive method of the three systems tested; however, the avidin-biotin method required the least amount of time. No significant difference in the quality of staining was observed between monoclonal antibodies and carefully absorbed conventional antiserum.

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