Host adaptation in the anther smut fungus Ustilago violacea (Microbotryum violaceum): infection success, spore production and alteration of floral traits on two host species and their F1-hybrid

Oecologia ◽  
1996 ◽  
Vol 107 (3) ◽  
pp. 307-320 ◽  
Author(s):  
Arjen Biere ◽  
Sonja Honders
Keyword(s):  
Host Species ◽  
Host Adaptation ◽  
Spore Production ◽  
Smut Fungus ◽  
Floral Traits ◽  
Microbotryum Violaceum ◽  
F1 Hybrid ◽  
Ustilago Violacea ◽  
Anther Smut

scholarly journals Mating System of the Anther Smut Fungus Microbotryum violaceum: Selfing under Heterothallism

2008 ◽  
Vol 7 (5) ◽  
pp. 765-775 ◽  
Author(s):  
Tatiana Giraud ◽  
Roxana Yockteng ◽  
Manuela López-Villavicencio ◽  
Guislaine Refrégier ◽  
Michael E. Hood
Keyword(s):  
Mating System ◽  
Smut Fungus ◽  
Microbotryum Violaceum ◽  
Anther Smut

Fimbrial-dependent mating inMicrobotryum violaceuminvolves a mannose–lectin interaction

1996 ◽  
Vol 42 (5) ◽  
pp. 461-466 ◽  
Author(s):  
Alan J. Castle ◽  
Nadia Stocco ◽  
Robert Boulianne
Keyword(s):  
Cell Adhesion ◽  
Concanavalin A ◽  
Smut Fungus ◽  
Mating Types ◽  
Carbohydrate Component ◽  
Microbotryum Violaceum ◽  
Anther Smut ◽  
Cell To Cell Adhesion ◽  
Fimbrial Protein ◽  
Dependent Mechanism

Fimbriae of the anther smut fungus, Microbotryum violaceum are polymers of six 74-kDa glycoprotein isoforms. Digestion of fimbrial monomers with α-mannosidase yielded two polypeptides with masses of 70 and 48 kDa. The 70-kDa polypeptide is probably a product of incomplete digestion and the 48-kDa polypeptide is the aglycone. Thus, most of the carbohydrate component of fimbrial protein is mannose. Previous observations have suggested that fimbriae are necessary for mating in M. violaceum. Further evidence for this role was obtained in the present study by showing that mating is inhibited by an anti-fimbrial protein antiserum, by mannose and related sugars glucose and arabinose, and by the lectin concanavalin A. Since inhibition was not complete, however, two mechanisms for adhesion between compatible cells were proposed, one fimbrial dependent and one independent. Lastly, fimbrial protein from a1but not a2mating types bound to a mannose–agarose column, suggesting a lectin-like capability. The fimbrial dependent mechanism of cell-to-cell adhesion may involve binding of the mannose residues of the fimbriae of a2cells by the fimbriae of a1cells.Key words: mating, Microbotryum violaceum, lectin, fimbriae.

Conjugation in Ustilago violacea. I. Morphology

1974 ◽  
Vol 20 (2) ◽  
pp. 187-191 ◽  
Author(s):  
N. H. Poon ◽  
J. Martin ◽  
A. W. Day
Keyword(s):  
Cell Cycle ◽  
Mating Type ◽  
Electron Micrographs ◽  
Cell Walls ◽  
Plasma Membranes ◽  
Smut Fungus ◽  
Type Ii ◽  
Opposite Mating Type ◽  
Ustilago Violacea ◽  
Anther Smut

Conjugation in the anther smut fungus, Ustilago violacea, is described and five stages are characterized viz. (i) intimate pairing of cells of opposite mating type; (ii) development of bumps from each cell at the point of pairing. The cell walls of opposing pegs are fused, but the plasma membranes are not yet affected; (iii) elongation of the bumps into pegs; (iv) dissolution of the walls and plasma membranes of opposing pegs at the point of contact, and the formation of a tube; (v) elongation of the tube to the mature mating configuration (about 5 μm). Electron micrographs and Nomarski interference contrast micrographs of this sequence are illustrated. The assembly of the conjugation tube begins as early as 1.5 h after the cells are mixed on mating medium and is completed in about 45 min. Even in asynchronous populations there is a burst of synchronous mating, followed by later asynchronous mating. Observations on the ability to mate of unbudded and budded cells support the evidence from cell cycle work that allele a1 mates only in the G1 phase (unbudded) while allele a2 is competent to mate during all phases.

Sign in / Sign up

Export Citation Format

Share Document