Desoxyribose nucleic acid (DNA) content and size of rat liver nuclei during thioac etamide intoxication and recovery

Chromosoma ◽  
1955 ◽  
Vol 7 (1) ◽  
pp. 281-327 ◽  
Author(s):  
Pauline Heizer
Keyword(s):  
Nucleic Acid ◽  
Rat Liver ◽  
Dna Content ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

scholarly journals DENSITY GRADIENT ISOLATION OF RAT LIVER NUCLEI WITH HIGH DNA CONTENT

1963 ◽  
Vol 17 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Richard F. Fisher ◽  
David J. Holbrook ◽  
J. Logan Irvin
Keyword(s):  
Density Gradient ◽  
Rat Liver ◽  
Dna Content ◽  
Phosphorus Content ◽  
Sucrose Solution ◽  
Normal Liver ◽  
Sucrose Density Gradient ◽  
Pellet Fraction ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

Rat liver nuclei, after preliminary isolation in 2.2 molar sucrose solution, were separated into density classes by centrifugation at 95,000 g for 45 to 85 minutes in a sucrose density gradient (density range, 1.28 to 1.33). Nuclei from normal liver separated into three bands with average DNA phosphorus content per nucleus of 0.67, 0.84, and 0.93 picogram for top, middle, and bottom bands, respectively. Nuclei from regenerating liver (26 hours after one-third hepatectomy) yielded three bands and a pellet fraction with average DNA phosphorus content per nucleus of 0.76, 1.02, 1.38, and 1.51 picograms (top to bottom of tube). This method appears capable of yielding nuclei which have increased their DNA content prior to mitosis, and this procedure should be valuable in studies of biochemical changes which occur in nuclei preparing for mitosis.

scholarly journals EFFECTS OF ALLOXAN AND REDUCED FOOD INTAKE ON LIVER DEOXYRIBOSE NUCLEIC ACID OF THE RAT

1959 ◽  
Vol 7 (2) ◽  
pp. 83-85 ◽  
Author(s):  
HENRY S. DI STEFANO ◽  
HAROLD F. DIERMEIER
Keyword(s):  
Nucleic Acid ◽  
Food Intake ◽  
Rat Liver ◽  
Liver Damage ◽  
Dna Content ◽  
Diabetic Rats ◽  
Deoxyribose Nucleic Acid ◽  
Liver Nuclei ◽  
Dna 2 ◽  
Complete Starvation

Effects of alloxan, reduced food intake, starvation and biopsy procedure on the deoxyribose nucleic acid (DNA) content of rat liver tetraploid nuclei have been investigated. It was found that: (1) Alloxan treatment resulted in an increased amount of DNA. (2) Reduced food intake of treated animals Played no part in this increase. (3) Complete starvation for a period of six days did not effect the amount of DNA in these nuclei. (4) An increased amount of DNA was found in tetraploid nuclei of rat liver 13 days after removal of a small biopsy sample. The increased DNA in tetraploid liver nuclei of alloxan diabetic rats may be explained on the basis of regeneration of liver tissue due to some submicroscopic liver damage caused by the drug.

Age And Polyploidy Of Rat Liver NUCLEI As Measured By Volume And DNA Content

1959 ◽  
Vol 14 (1) ◽  
pp. 2-8 ◽  
Author(s):  
J. A. Falzone ◽  
C. H. Barrows ◽  
N. W. Shock
Keyword(s):  
Rat Liver ◽  
Dna Content ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

scholarly journals Modulation by Exogenous Histones of Phosphorylation of Non-Histone Nuclear Proteins in Isolated Rat Liver Nuclei

1973 ◽  
Vol 248 (21) ◽  
pp. 7595-7600
Author(s):  
Edward M. Johnson ◽  
Giorgio Vidali ◽  
Virginia C. Littau ◽  
Vincent G. Allfrey
Keyword(s):  
Rat Liver ◽  
Nuclear Proteins ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat
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