scholarly journals EFFECTS OF ALLOXAN AND REDUCED FOOD INTAKE ON LIVER DEOXYRIBOSE NUCLEIC ACID OF THE RAT

1959 ◽  
Vol 7 (2) ◽  
pp. 83-85 ◽  
Author(s):  
HENRY S. DI STEFANO ◽  
HAROLD F. DIERMEIER
Keyword(s):  
Nucleic Acid ◽  
Food Intake ◽  
Rat Liver ◽  
Liver Damage ◽  
Dna Content ◽  
Diabetic Rats ◽  
Deoxyribose Nucleic Acid ◽  
Liver Nuclei ◽  
Dna 2 ◽  
Complete Starvation

Effects of alloxan, reduced food intake, starvation and biopsy procedure on the deoxyribose nucleic acid (DNA) content of rat liver tetraploid nuclei have been investigated. It was found that: (1) Alloxan treatment resulted in an increased amount of DNA. (2) Reduced food intake of treated animals Played no part in this increase. (3) Complete starvation for a period of six days did not effect the amount of DNA in these nuclei. (4) An increased amount of DNA was found in tetraploid nuclei of rat liver 13 days after removal of a small biopsy sample. The increased DNA in tetraploid liver nuclei of alloxan diabetic rats may be explained on the basis of regeneration of liver tissue due to some submicroscopic liver damage caused by the drug.

STUDY OF SOME FACTORS THAT AFFECT THE DISTRIBUTION OF NUCLEAR VOLUMES IN PREPARATIONS OF RAT LIVER NUCLEI

1967 ◽  
Vol 45 (7) ◽  
pp. 1175-1183 ◽  
Author(s):  
Roberto Umaña
Keyword(s):  
Rat Liver ◽  
Dna Content ◽  
Direct Relationship ◽  
Magnesium Ion ◽  
Homogenization Procedure ◽  
Ion Concentrations ◽  
Ph Values ◽  
Sucrose Solutions ◽  
Liver Nuclei ◽  
Calcium And Magnesium

The effect of the homogenization procedure, the centrifugation scheme, and the composition of the suspension medium on the distribution of nuclear volumes has been studied.It has been shown that the Waring Blendor not only destroys a greater number of the nuclei during homogenization, but also that this destruction is a selective one. At neutral pH values, no direct relationship appears to exist between the DNA content of the nuclei and their density. For this reason, purification in concentrated sucrose solutions produces a selective loss of the lighter nuclei, which includes small diploid stromal nuclei and some of the larger polyploid type of parenchymal nuclei.The study of the effect of increasing the calcium and magnesium ion concentrations (from 0.001 to 0.005 M) on the nuclear distribution showed that these ions produce a selective shrinkage and condensation of the nuclei, probably through different mechanisms.

scholarly journals DENSITY GRADIENT ISOLATION OF RAT LIVER NUCLEI WITH HIGH DNA CONTENT

1963 ◽  
Vol 17 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Richard F. Fisher ◽  
David J. Holbrook ◽  
J. Logan Irvin
Keyword(s):  
Density Gradient ◽  
Rat Liver ◽  
Dna Content ◽  
Phosphorus Content ◽  
Sucrose Solution ◽  
Normal Liver ◽  
Sucrose Density Gradient ◽  
Pellet Fraction ◽  
Rat Liver Nuclei ◽  
Liver Nuclei

Rat liver nuclei, after preliminary isolation in 2.2 molar sucrose solution, were separated into density classes by centrifugation at 95,000 g for 45 to 85 minutes in a sucrose density gradient (density range, 1.28 to 1.33). Nuclei from normal liver separated into three bands with average DNA phosphorus content per nucleus of 0.67, 0.84, and 0.93 picogram for top, middle, and bottom bands, respectively. Nuclei from regenerating liver (26 hours after one-third hepatectomy) yielded three bands and a pellet fraction with average DNA phosphorus content per nucleus of 0.76, 1.02, 1.38, and 1.51 picograms (top to bottom of tube). This method appears capable of yielding nuclei which have increased their DNA content prior to mitosis, and this procedure should be valuable in studies of biochemical changes which occur in nuclei preparing for mitosis.

Age And Polyploidy Of Rat Liver NUCLEI As Measured By Volume And DNA Content

1959 ◽  
Vol 14 (1) ◽  
pp. 2-8 ◽  
Author(s):  
J. A. Falzone ◽  
C. H. Barrows ◽  
N. W. Shock
Keyword(s):  
Rat Liver ◽  
Dna Content ◽  
Rat Liver Nuclei ◽  
Liver Nuclei
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