The soybean chloroplast genome: Construction of a circular restriction site map and location of DNA regions encoding the genes for rRNAs, the large subunit of the ribulose-1,5-bisphosphate carboxylase and the 32 KD protein of the photosystem II reaction center

1983 ◽  
Vol 190 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Albert Spielmann ◽  
William Ortiz ◽  
Erhard Stutz
Keyword(s):  
Photosystem Ii ◽  
Chloroplast Genome ◽  
Reaction Center ◽  
Restriction Site ◽  
Large Subunit ◽  
Bisphosphate Carboxylase ◽  
Restriction Site Map

scholarly journals Chloroplast Genome Evolution in the Genus Avena

Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 613-621
Author(s):  
Koji Murai ◽  
Koichiro Tsunewaki
Keyword(s):  
Chloroplast Genome ◽  
Restriction Site ◽  
Physical Map ◽  
Fragment Size ◽  
Large Subunit ◽  
Single Copy ◽  
Rbcl Gene ◽  
Type I ◽  
Bisphosphate Carboxylase ◽  
Chloroplast Genomes

ABSTRACT The genus Avena contains five different chloroplast genomes, I-V. A physical map of chloroplast (ct) DNA of Avena sativa (type I chloroplast genome) was constructed using three restriction endonucleases, PstI, SalI and SmaI. This genome is ca. 135.5 kbp in size, and contains two inverted repeats of ca. 22.5 kbp each, separated by a large (ca. 79.0 kbp) and small (ca. 12.5 kbp) single copy region. The rbcL gene which codes for the large subunit of ribulose 1,5-bisphosphate carboxylase, was located in the map. Restriction fragment patterns of all five chloroplast genomes were compared, and among them five fragment size and five restriction site mutations were disclosed. Four site mutations were found in two or more chloroplast genomes, the other site and five fragment size mutations were specific to one or another of the chloroplast genomes. A dendrogram showing phylogenetic relationships among the five chloroplast genomes, based on the distribution of the common and specific mutations among them, indicates that chloroplast genome divergence characterized by three restriction site mutations occurred first between two diploid groups, each carrying A and C genome (nuclear), respectively, followed by further speciation in each group.

scholarly journals A new photosystem II reaction center component (4.8 kDa protein) encoded by chloroplast genome

FEBS Letters ◽  
1988 ◽  
Vol 241 (1-2) ◽  
pp. 99-104 ◽  
Author(s):  
Masahiko Ikeuchi ◽  
Yorinao Inoue
Keyword(s):  
Photosystem Ii ◽  
Chloroplast Genome ◽  
Reaction Center

Somatic hybridization between Nicotiana rustica and N. tabacum. IV. Analysis of nuclear and chloroplast genome expression in somatic hybrids

1981 ◽  
Vol 59 (8) ◽  
pp. 1509-1513 ◽  
Author(s):  
G. C. Douglas ◽  
L. R. Wetter ◽  
W. A. Keller ◽  
G. Setterfield
Keyword(s):  
Chloroplast Genome ◽  
Banding Pattern ◽  
Parental Species ◽  
Somatic Hybrids ◽  
Large Subunit ◽  
Small Subunit ◽  
Bisphosphate Carboxylase ◽  
Nicotiana Rustica ◽  
Genome Expression ◽  
Hybrid Plants

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RUBPCase) from 14 Nicotiana rustica + N. tabacum somatic hybrid plants was isolated and subjected to isoelectric focussing after carboxymethylation. The resulting polypeptide banding pattern for the nuclear-encoded small subunit of the enzyme showed bands derived from both parental species whereas the banding pattern for the plastid-encoded large subunit showed bands characteristic of N. rustica or N. tabacum but not both species. Eleven plants expressed the chloroplast genome of N. rustica and three expressed N. tabacum chloroplast genes.

scholarly journals Regulation of genes encoding the large subunit of ribulose-1,5-bisphosphate carboxylase and the photosystem II polypeptides D-1 and D-2 during the cell cycle of Chlamydomonas reinhardtii.

1986 ◽  
Vol 103 (5) ◽  
pp. 1837-1845 ◽  
Author(s):  
D L Herrin ◽  
A S Michaels ◽  
A L Paul
Keyword(s):  
Cell Cycle ◽  
Photosystem Ii ◽  
Dark Period ◽  
Large Subunit ◽  
Light Period ◽  
Mrna Levels ◽  
Bisphosphate Carboxylase ◽  
Polypeptide Synthesis ◽  
Translational Level

Synthesis of the major chloroplast proteins is temporally regulated in light-dark-synchronized Chlamydomonas cells. We have used cloned chloroplast DNA probes, and in vitro and in vivo protein synthesis to examine the cell cycle regulation of photosystem II polypeptides D-1 and D-2, and the large subunit of ribulose-1,5-bisphosphate carboxylase (RuBPCase LS). Synthesis and accumulation of D-1 and D-2 mRNAs occurs during the first half of the light period (G1), correlating with increasing synthesis of the polypeptides. Rifampicin, added immediately before the light period, inhibited the normal increase in D-1, D-2 polypeptide synthesis. During the dark period D-1, D-2 mRNAs persist at high levels despite reduced rates of mRNA synthesis and translation during this period. Cell-free translation analyses indicate that the D-1 mRNA present during the dark period is efficient at directing synthesis of the D-1 precursor in vitro. We conclude that expression of the psbA (D-1) and psbD (D-2) genes are regulated primarily at the transcriptional level during the light-induction period but at the translational level for the remainder of the cell cycle. Transcripts of the RuBPCase LS gene (rbcL) are also found at high levels during the light and dark periods but, unlike D-1 and D-2, LS mRNA levels do not increase until the last half of the light period and measurable synthesis and accumulation of this mRNA occurs during the dark. Furthermore, induction of LS polypeptide synthesis during the light period is insensitive to rifampicin. We conclude that LS production is regulated primarily at the translational level during the cell cycle.

scholarly journals Endosymbiotic chloroplasts in molluscan cells contain proteins synthesized after plastid capture

1996 ◽  
Vol 199 (10) ◽  
pp. 2323-2330 ◽  
Author(s):  
S Pierce ◽  
R Biron ◽  
M Rumpho
Keyword(s):  
Protein Synthesis ◽  
Photosystem Ii ◽  
Host Cell ◽  
Protein Turnover ◽  
Large Subunit ◽  
Protein Synthesis Inhibitors ◽  
Bisphosphate Carboxylase ◽  
Elysia Chlorotica ◽  
Associated Proteins ◽  
Plastid Protein

Endosymbiotic chloroplasts within the cells of the ascoglossan slug Elysia chlorotica synthesize a variety of proteins including the large subunit of ribulose-1,5-bisphosphate-carboxylase oxygenase (RuBisCO) and the photosystem II protein D1. In addition, the effects of protein synthesis inhibitors suggest that some chloroplast-associated proteins are synthesized in the animal cytosol and subsequently translocated into the chloroplasts. Thus, the plastids not only synthesize proteins during this long-lived association, but the host cell seems to play a role in plastid protein turnover.

Sign in / Sign up

Export Citation Format

Share Document