pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors

1982 ◽  
Vol 185 (2) ◽  
pp. 223-238 ◽  
Author(s):  
Tobias Kieser ◽  
David A. Hopwood ◽  
Helen M. Wright ◽  
Charles J. Thompson
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Broad Host Range ◽  
Cloning Vectors ◽  
Dna Cloning

Broad-host-range cloning vectors derived from the W-plasmid Sa

Gene ◽  
1982 ◽  
Vol 19 (3) ◽  
pp. 361-364 ◽  
Author(s):  
J. Leemans ◽  
J. Langenakens ◽  
H. De Greve ◽  
R. Deblaere ◽  
M. Van Montagu ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Cloning Vectors

A broad host range food-grade cloning vector for lactic acid bacteria

Biologia ◽  
2016 ◽  
Vol 71 (5) ◽  
Author(s):  
Parichat Phumkhachorn ◽  
Pongsak Rattanachaikunsopon
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Host Range ◽  
Genetic Modification ◽  
Cloning Vector ◽  
Broad Host Range ◽  
Cloning Vectors ◽  
Food Grade ◽  
Food Industries

AbstractThe genetic modification of lactic acid bacteria being used in medicine and food industries has been limited due to the scarcity of food-grade cloning vectors for the bacteria. The 4.46-kb food-grade cloning vector pUBU constructed in this study consisted of 3 major components from food-approved organisms, the theta-type replicon from pUCL287 of

Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria

Gene ◽  
1988 ◽  
Vol 70 (1) ◽  
pp. 191-197 ◽  
Author(s):  
N.T. Keen ◽  
S. Tamaki ◽  
D. Kobayashi ◽  
D. Trollinger
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Dna Cloning ◽  
Gram Negative ◽  
Broad Host Range Plasmids

scholarly journals Functional Analysis of Three Plasmids from Lactobacillus plantarum

2005 ◽  
Vol 71 (3) ◽  
pp. 1223-1230 ◽  
Author(s):  
Richard van Kranenburg ◽  
Natasa Golic ◽  
Roger Bongers ◽  
Rob J. Leer ◽  
Willem M. de Vos ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Bacillus Subtilis ◽  
Host Range ◽  
Lactobacillus Plantarum ◽  
Unknown Function ◽  
Conjugative Plasmid ◽  
Rolling Circle Replication ◽  
Cloning Vectors ◽  
Rolling Circle ◽  
Arsenite Resistance

ABSTRACT Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.

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