Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae

1992 ◽  
Vol 21 (4-5) ◽  
pp. 319-324 ◽  
Author(s):  
Eckard Haase ◽  
J�rg Servos ◽  
Martin Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of omnipotent suppressors in the yeast Saccharomyces cerevisiae.

Genetics ◽  
1990 ◽  
Vol 124 (3) ◽  
pp. 515-522
Author(s):  
L P Wakem ◽  
F Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Analysis ◽  
Low Temperatures ◽  
Carbon Sources ◽  
Yeast Saccharomyces Cerevisiae ◽  
Hypertonic Medium ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Chromosome Ii

Abstract Approximately 290 omnipotent suppressors, which enhance translational misreading, were isolated in strains of the yeast Saccharomyces cerevisiae containing the psi+ extrachromosomal determinant. The suppressors could be assigned to 8 classes by their pattern of suppression of five nutritional markers. The suppressors were further distinguished by differences in growth on paromomycin medium, hypertonic medium, low temperatures (10 degrees), nonfermentable carbon sources, alpha-aminoadipic acid medium, and by their dominance and recessiveness. Genetic analysis of 12 representative suppressors resulted in the assignment of these suppressors to 6 different loci, including the three previously described loci SUP35 (chromosome IV), SUP45 (chromosome II) and SUP46 (chromosome II), as well as three new loci SUP42 (chromosome IV), SUP43 (chromosome XV) and SUP44 (chromosome VII). Suppressors belonging to the same locus had a wide range of different phenotypes. Differences between alleles of the same locus and similarities between alleles of different loci suggest that the omnipotent suppressors encode proteins that effect different functions and that altered forms of each of the proteins can effect the same function.

Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae

1989 ◽  
Vol 9 (9) ◽  
pp. 3638-3646
Author(s):  
C M Nicolet ◽  
E A Craig
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Two Dimensional Gel Electrophoresis ◽  
Reading Frame ◽  
Hsp70 Family ◽  
Isolation And Characterization ◽  
Isoelectric Points ◽  
Amino Acid Analog ◽  
Inducible Gene

We have isolated a gene from the yeast Saccharomyces cerevisiae that encodes a 2.0-kilobase heat-inducible mRNA. This gene, which we have designated STI1, for stress inducible, was also induced by the amino acid analog canavanine and showed a slight increase in expression as cells moved into stationary phase. The STI1 gene encodes a 66-kilodalton protein, as determined from the sequence of the longest open reading frame. The putative STI1 protein, as identified by two-dimensional gel electrophoresis, migrated in the region of 73 to 75 kilodaltons as a series of four isoforms with different isoelectric points. STI1 is not homologous to the other conserved HSP70 family members in yeasts, despite similarities in size and regulation. Cells carrying a disruption mutation of the STI1 gene grew normally at 30 degrees C but showed impaired growth at higher and lower temperatures. Overexpression of the STI1 gene resulted in substantial trans-activation of SSA4 promoter-reporter gene fusions, indicating that STI1 may play a role in mediating the heat shock response of some HSP70 genes.

scholarly journals Isolation and characterization of actin-like protein from yeast Saccharomyces Cerevisiae

FEBS Letters ◽  
1979 ◽  
Vol 102 (1) ◽  
pp. 55-58 ◽  
Author(s):  
V.E. Koteliansky ◽  
M.A. Glukhova ◽  
M.V. Bejanian ◽  
A.P. Surguchov ◽  
V.N. Smirnov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Isolation And Characterization

Isolation and Characterization of WHI3, a Size-Control Gene of Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1469-1480 ◽  
Author(s):  
R S Nash ◽  
T Volpe ◽  
B Futcher
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Binding ◽  
Size Control ◽  
Binding Domain ◽  
Small Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Isolation And Characterization ◽  
Rna Binding Domain ◽  
Mating Factor

Abstract WHI3 is a gene affecting size control and cell cycle in the yeast Saccharomyces cerevisiae. The whi3 mutant has small cells, while extra doses of WHI3 produce large cells, and a large excess of WHI3 produces a lethal arrest in G1 phase. WHI3 seems to be a dose-dependent inhibitor of Start. Whi3 and its partially redundant homolog Whi4 have an RNA-binding domain, and mutagenesis experiments indicate that this RNA-binding domain is essential for Whi3 function. CLN3-1 whi3 cells are extremely small, nearly sterile, and largely nonresponsive to mating factor. Fertility is restored by deletion of CLN2, suggesting that whi3 cells may have abnormally high levels of CLN2 function.

Sign in / Sign up

Export Citation Format

Share Document