Context-dependent transcriptional regulations of YAP/TAZ in stem cell and differentiation

Hippo pathway is initially identified as a master regulator for cell proliferation and organ size control, and the subsequent researches show this pathway is also involved in development, tissue regeneration and homeostasis, inflammation, immunity and cancer. YAP/TAZ, the downstream effectors of Hippo pathway, usually act as coactivators and are dependent on other transcription factors to mediate their transcriptional outputs. In this review, we will first provide an overview on the core components and regulations of Hippo pathway in mammals, and then systematically summarize the identified transcriptional factors or partners that are responsible for the transcriptional output of YAP/TAZ in stem cell and differentiation. More than that, we will discuss the potential applications and future directions based on these findings.

Adaptive time step algorithms for the simulation of marine ecosystem models using the transport matrix method implementation Metos3D (v0.5.0)

The reduction of the computational effort is desirable for the simulation of marine ecosystem models. Using a marine ecosystem model, the assessment and the validation of annual periodic solutions (i.e., steady annual cycles) against observational data are crucial to identify biogeochemical processes, which, for example, influence the global carbon cycle. For marine ecosystem models, the transport matrix method (TMM) already lowers the runtime of the simulation significantly and enables the application of larger time steps straightforwardly. However, the selection of an appropriate time step is a challenging compromise between accuracy and shortening the runtime. Using an automatic time step adjustment during the computation of a steady annual cycle with the TMM, we present in this paper different algorithms applying either an adaptive step size control or decreasing time steps in order to use the time step always as large as possible without any manual selection. For these methods and a variety of marine ecosystem models of different complexity, the accuracy of the computed steady annual cycle achieved the same accuracy as solutions obtained with a fixed time step. Depending on the complexity of the marine ecosystem model, the application of the methods shortened the runtime significantly. Due to the certain overhead of the adaptive method, the computational effort may be higher in special cases using the adaptive step size control. The presented methods represent computational efficient methods for the simulation of marine ecosystem models using the TMM but without any manual selection of the time step.

Branching processes with resetting as a model for cell division

We study the Stochastic Thermodynamics of cell growth and division using a theoretical framework based on branching processes with resetting. Cell division may be split into two sub-processes: branching, by which a given cell gives birth to an identical copy of itself, and resetting, by which some properties of the daughter cells (such as their size or age) are reset to new values following division. We derive the first and second laws of Stochastic Thermodynamics for this process, and identify separate contributions due to branching and resetting. We apply our framework to well-known models of cell size control, such as the sizer, the timer, and the adder. We show that the entropy production of resetting is negative and that of branching is positive for these models in the regime of exponential growth of the colony. This property suggests an analogy between our model for cell growth and division and heat engines, and the introduction of a thermodynamic efficiency, which quantifies the conversion of one form of entropy production to another.

Effect of Grain Size on Change in Surface Roughness of Carbon Steels Through Polishing Processes

A mirror-like reflecting surface is an important characteristic in many industrial metallic parts. Polishing is done to form a mirror surface on metals. However, the effect of the grain size of metals on surface roughness through polishing processes is not clear. Specifically, mirror surface formation of ultrafine grained materials is still unknown. Ultrafine grained steels and coarse grained steels with 0.02, 0.10, and 0.60 wt% carbon contents were prepared by warm caliber rolling and annealing. Average grain sizes were 1–2 μm and 4–40 μm. The changes in surface roughness, Sa, were measured with an atomic force microscope (AFM) via eight polishing steps, using emery papers of type #600, #1000, #1500, #2000, #2500, #4000, and free abrasive grains of 3 μm and 1 μm diamond. As the polishing process progressed, the surface unevenness was removed and the surface roughness, Sa, decreased in all steels. The differences of Sa at each polishing step were analyzed from the point of carbon content, Vickers hardness, and grain size. Carbon contents and Vickers hardness have little effect on Sa. However, grain size has a considerable effect on Sa in all steels. Ultrafine grained steels have smaller Sa in all polishing steps in all steels. This is because ultrafine grained steels have very small work hardening rate. After final polishing, Sa is 2.5–3.6 nm in coarse grained steels and 2.0–2.6 nm in ultrafine grained steels. To obtain a mirror surface with smaller Sa, grain size control is important.

Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division

Fission yeast cells prevent mitotic entry until a threshold cell surface area is reached. The protein kinase Cdr2 contributes to this size control system by forming multiprotein nodes that inhibit Wee1 at the medial cell cortex. Cdr2 node anchoring at the cell cortex is not fully understood. Through a genomic screen, we identified the conserved GTPase Arf6 as a component of Cdr2 signaling. Cells lacking Arf6 failed to divide at a threshold surface area and instead shifted to volume-based divisions at increased overall size. Arf6 stably localized to Cdr2 nodes in its GTP-bound but not GDP-bound state, and its guanine nucleotide exchange factor (GEF), Syt22, was required for both Arf6 node localization and proper size at division. In arf6Δ mutants, Cdr2 nodes detached from the membrane and exhibited increased dynamics. These defects were enhanced when arf6Δ was combined with other node mutants. Our work identifies a regulated anchor for Cdr2 nodes that is required for cells to sense surface area.

Realizing Shape and Size Control for the Synthesis of Coordination Polymer Nanoparticles templated by Diblock Copolymer Micelles

The combination of polymers with nanoparticles offers the possibility to obtain customizable composite materials with additional properties such as sensing or bistability provided by a switchable spin crossover (SCO) core. For all applications, a precise control over size and shape of the nanomaterial is highly important as it will significantly influence its final properties. By confined synthesis of iron(II) SCO coordination polymers within the P4VP cores of polystyrene-block-poly-4-vinylpyridine (PS-b-P4VP) micelles in THF we are able to control the size and also the shape of the resulting SCO nanocomposite particles by the composition of the PS-b-P4VP diblock copolymers (dBCPs) and the amount of complex employed. For the nanocomposite samples with the highest P4VP content, a morphological transition from spherical nanoparticle to worm-like structures was observed with increasing coordination polymer content, which can be explained with the impact of complex coordination on the self-assembly of the dBCP. Furthermore, the SCO nanocomposites showed transition temperatures of T1/2 = 217 K, up to 27 K wide hysteresis loops and a decrease of the residual high-spin fraction down to γHS = 14% in the worm-like structures, as determined by magnetic susceptibility measurements and Mössbauer spectroscopy. Thus, SCO properties close or even better (hysteresis) to those of the bulk material can be obtained and furthermore tuned through size and shape control realized by tailoring the block length ratio of the PS-b-P4VP dBCPs.