Plasmid transformation in Bacillus subtilis

G. Bensi; A. Iglesias; U. Canosi; T. A. Trautner

doi:10.1007/bf00352512

Plasmid transformation in Bacillus subtilis

MGG Molecular & General Genetics ◽

10.1007/bf00352512 ◽

1981 ◽

Vol 184 (3) ◽

pp. 400-404 ◽

Cited By ~ 18

Author(s):

G. Bensi ◽

A. Iglesias ◽

U. Canosi ◽

T. A. Trautner

Keyword(s):

Bacillus Subtilis ◽

Plasmid Transformation

Restriction and modification in Bacillus subtilis Marburg 168: Target sites and effects on plasmid transformation

MGG Molecular & General Genetics ◽

10.1007/bf00338412 ◽

1988 ◽

Vol 211 (1) ◽

pp. 186-189 ◽

Cited By ~ 16

Author(s):

S. Bron ◽

L. Jannière ◽

S. D. Ehrlich

Keyword(s):

Bacillus Subtilis ◽

Plasmid Transformation ◽

Target Sites

Intramolecular recombination during plasmid transformation of Bacillus subtilis competent cells.

The EMBO Journal ◽

10.1002/j.1460-2075.1982.tb01356.x ◽

1982 ◽

Vol 1 (12) ◽

pp. 1565-1571 ◽

Cited By ~ 18

Author(s):

B. Michel ◽

B. Niaudet ◽

S.D. Ehrlich

Keyword(s):

Bacillus Subtilis ◽

Plasmid Transformation ◽

Competent Cells ◽

Intramolecular Recombination

RecA Regulation by RecU and DprA During Bacillus subtilis Natural Plasmid Transformation

Frontiers in Microbiology ◽

10.3389/fmicb.2018.01514 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 8

Author(s):

Ester Serrano ◽

Begoña Carrasco ◽

Jamie L. Gilmore ◽

Kunio Takeyasu ◽

Juan C. Alonso

Keyword(s):

Bacillus Subtilis ◽

Plasmid Transformation

Genetic Recombination in Bacillus subtilis 168: Effect of ΔhelD on DNA Repair and Homologous Recombination

Journal of Bacteriology ◽

10.1128/jb.183.19.5772-5777.2001 ◽

2001 ◽

Vol 183 (19) ◽

pp. 5772-5777 ◽

Cited By ~ 14

Author(s):

Begoña Carrasco ◽

Silvia Fernández ◽

Marie-Agnes Petit ◽

Juan C. Alonso

Keyword(s):

Dna Repair ◽

Bacillus Subtilis ◽

Homologous Recombination ◽

Genetic Recombination ◽

Methyl Methanesulfonate ◽

Plasmid Transformation ◽

Dna Damaging Agents ◽

Bacillus Subtilis 168 ◽

Recombination Repair ◽

Helicase Iv

ABSTRACT The B. subtilis ΔhelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec+ strain. The ΔhelD allele was introduced into rec-deficient strains representative of the α (recF strain), β (addA addB), γ (recH), ɛ (ΔrecU), and ζ (ΔrecS) epistatic groups. The ΔhelDmutation increased the sensitivity to DNA-damaging agents ofaddAB, ΔrecU, and ΔrecS cells, did not affect the survival ofrecH cells, and decreased the sensitivity ofrecF cells. ΔhelD also partially suppressed the DNA repair phenotype of other mutations classified within the α epistatic group, namely the recL, ΔrecO, and recR mutations. The ΔhelD allele marginally reduced plasmid transformation (three- to sevenfold) of mutations classified within the α, β, and γ epistatic groups. Altogether, these data indicate that the loss of helicase IV might stabilize recombination repair intermediates formed in the absence of recFLOR and renderrecFLOR, addAB, andrecH cells impaired in plasmid transformation.