Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors

1988 ◽  
Vol 1 (4) ◽  
pp. 319-329 ◽  
Author(s):  
Volker J�ger ◽  
J�rgen Lehmann ◽  
Peter Friedl
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Wide Spectrum ◽  
Serum Free ◽  
Free Growth ◽  
Stirred Bioreactors

Serum-Free Growth Medium Sustains Commitment of Human Articular Chondrocyte through Maintenance of Sox9 Expression

2004 ◽  
Vol 10 (1-2) ◽  
pp. 145-155 ◽  
Author(s):  
Mara Malpeli ◽  
Nadia Randazzo ◽  
Ranieri Cancedda ◽  
Beatrice Dozin
Keyword(s):  
Growth Medium ◽  
Articular Chondrocyte ◽  
Sox9 Expression ◽  
Serum Free ◽  
Free Growth

Chicken egg yolk-supplemented medium and the serum-free growth of normal mammalian cells

In Vitro ◽  
1983 ◽  
Vol 19 (11) ◽  
pp. 811-817 ◽  
Author(s):  
D. K. Fujii ◽  
D. Gospodarowicz
Keyword(s):  
Mammalian Cells ◽  
Egg Yolk ◽  
Serum Free ◽  
Chicken Egg ◽  
Free Growth ◽  
Chicken Egg Yolk

Ganglioside alterations in YAC-1 cells cultivated in serum-supplemented and serum-free growth medium

1992 ◽  
Vol 9 (5) ◽  
pp. 265-273 ◽  
Author(s):  
Johannes M�thing ◽  
Antje P�rtner ◽  
Volker J�ger
Keyword(s):  
Growth Medium ◽  
Serum Free ◽  
Free Growth

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

scholarly journals Primary Cilium in Cancer Hallmarks

2019 ◽  
Vol 20 (6) ◽  
pp. 1336 ◽  
Author(s):  
Lucilla Fabbri ◽  
Frédéric Bost ◽  
Nathalie Mazure
Keyword(s):  
Cancer Progression ◽  
Primary Cilium ◽  
Mammalian Cells ◽  
Current Knowledge ◽  
Wide Spectrum ◽  
Cancer Hallmarks ◽  
Mutual Regulation ◽  
Ultrastructure And Function ◽  
And Function ◽  
External Stimuli

The primary cilium is a solitary, nonmotile and transitory appendage that is present in virtually all mammalian cells. Our knowledge of its ultrastructure and function is the result of more than fifty years of research that has dramatically changed our perspectives on the primary cilium. The mutual regulation between ciliogenesis and the cell cycle is now well-recognized, as well as the function of the primary cilium as a cellular “antenna” for perceiving external stimuli, such as light, odorants, and fluids. By displaying receptors and signaling molecules, the primary cilium is also a key coordinator of signaling pathways that converts extracellular cues into cellular responses. Given its critical tasks, any defects in primary cilium formation or function lead to a wide spectrum of diseases collectively called “ciliopathies”. An emerging role of primary cilium is in the regulation of cancer development. In this review, we seek to describe the current knowledge about the influence of the primary cilium in cancer progression, with a focus on some of the events that cancers need to face to sustain survival and growth in hypoxic microenvironment: the cancer hallmarks.

A Serum-Free, Transient Transfection System for Enhancing Production of Recombinant Antibodies in Mammalian Cells

2010 ◽  
pp. 229-232
Author(s):  
Gaurav Backliwal ◽  
Sarah Wulhfard ◽  
Fanny Delegrange ◽  
Lucia Baldi ◽  
Maria de Jesus ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Recombinant Antibodies ◽  
Transient Transfection ◽  
Serum Free ◽  
Enhancing Production
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