Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.)

Planta ◽  
1980 ◽  
Vol 148 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Ronald R. D. Croy ◽  
John A. Gatehouse ◽  
I. Marta Evans ◽  
Donald Boulter
Planta ◽  
1980 ◽  
Vol 148 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Ronald R. D. Croy ◽  
John A. Gatehouse ◽  
I. Marta Evans ◽  
Donald Boulter

Planta ◽  
1979 ◽  
Vol 144 (5) ◽  
pp. 455-462 ◽  
Author(s):  
I. Marta Evans ◽  
Ronald R. D. Croy ◽  
Philippa Hutchinson ◽  
Donald Boulter ◽  
Peter I. Payne ◽  
...  

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.


2016 ◽  
Vol 68 (7-8) ◽  
pp. 762-770 ◽  
Author(s):  
Miaomiao Shi ◽  
Kai Wang ◽  
Shujuan Yu ◽  
Robert G. Gilbert ◽  
Qunyu Gao

Heredity ◽  
1984 ◽  
Vol 53 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Sayed H Mahmoud ◽  
John A Gatehouse

2020 ◽  
Vol 72 (9-10) ◽  
pp. 1900228 ◽  
Author(s):  
Manolo Gonzalez ◽  
Jose Alvarez‐Ramirez ◽  
E. Jaime Vernon‐Carter ◽  
Isabel Reyes ◽  
Lurdes Alvarez‐Poblano

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