Grafting in vitro Regenerated Pea (Pisum sativum L.) Shoots as a Method for Obtaining Mature Plants

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

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Structural characterizations and in vitro digestibility of acid-treated wrinkled and smooth pea starch (Pisum sativum L.)

Starch - Stärke ◽

10.1002/star.201500260 ◽

2016 ◽

Vol 68 (7-8) ◽

pp. 762-770 ◽

Cited By ~ 7

Author(s):

Miaomiao Shi ◽

Kai Wang ◽

Shujuan Yu ◽

Robert G. Gilbert ◽

Qunyu Gao

Keyword(s):

Pisum Sativum ◽

In Vitro Digestibility ◽

Pisum Sativum L ◽

Pea Starch ◽

Structural Characterizations

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In-Vitro Translation and Processing of Compartment-Specific Glucan Phosphorylase Isozymes from Pisum Sativum L.

Current Research in Photosynthesis ◽

10.1007/978-94-009-0511-5_567 ◽

1990 ◽

pp. 2511-2514

Author(s):

Martin Steup ◽

Jochen van Berkel ◽

Jutta Conrads-Strauch

Keyword(s):

Pisum Sativum ◽

In Vitro Translation ◽

Pisum Sativum L ◽

Glucan Phosphorylase

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Effect of the Drying Temperature on Color, Antioxidant Activity and In Vitro Digestibility of Green Pea ( Pisum sativum L.) Flour

Starch - Stärke ◽

10.1002/star.201900228 ◽

2020 ◽

Vol 72 (9-10) ◽

pp. 1900228 ◽

Cited By ~ 1

Author(s):

Manolo Gonzalez ◽

Jose Alvarez‐Ramirez ◽

E. Jaime Vernon‐Carter ◽

Isabel Reyes ◽

Lurdes Alvarez‐Poblano

Keyword(s):

Antioxidant Activity ◽

Pisum Sativum ◽

In Vitro Digestibility ◽

Drying Temperature ◽

Pisum Sativum L ◽

Green Pea

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Physicochemical properties and in vitro digestibility of flour and starch from pea (Pisum sativum L.) cultivars

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2011.10.004 ◽

2012 ◽

Vol 50 (1) ◽

pp. 131-137 ◽

Cited By ~ 31

Author(s):

Hyun-Jung Chung ◽

Qiang Liu

Keyword(s):

Pisum Sativum ◽

Physicochemical Properties ◽

In Vitro Digestibility ◽

Pisum Sativum L

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Morphogenesis of the compound leaf in three genotypes of the pea, Pisum sativum

Canadian Journal of Botany ◽

10.1139/b86-175 ◽

1986 ◽

Vol 64 (6) ◽

pp. 1268-1276 ◽

Cited By ~ 25

Author(s):

K. S. Gould ◽

Elizabeth G. Cutter ◽

J. P. W. Young

Keyword(s):

Pisum Sativum ◽

Leaf Development ◽

Culture System ◽

Algebraic Model ◽

Leaf Primordium ◽

Axillary Shoots ◽

Compound Leaf ◽

Pisum Sativum L ◽

Leaflet Anatomy

Leaf anatomy, ontogeny, and morphology were described and compared in a pea line (Pisum sativum L.) with conventional leaves and in isogenic lines carrying the mutations af (afila) or tl (tendril-less or acacia). The anatomy of stem, petiole, and rachis is not modified by these mutations. The tendrils, which in af replace leaflets, have normal tendril anatomy, and the terminal leaflets of the tl form have normal leaflet anatomy. The shoot apical dome has the same size and shape in the three genotypes, as does the leaf primordium up to the stage of initiation of the first laterals. The mature morphology of leaves varies with node of insertion. Some leaves, especially at nodes 3 and 4, have structures that are not typical of their genotype. An in vitro culture system is described for axillary shoots. Such shoots recapitulate most of the foliar features of seedling plants, but leaf morphology is on average more complex, and aberrant structures are more frequent. All these observations are discussed in relation to Young's algebraic model for compound leaf development.

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