Plasmid transformation in Bacillus subtilis: Effects of insertion of Bacillus subtilis DNA into plasmid pC194

1981 ◽  
Vol 181 (4) ◽  
pp. 434-440 ◽  
Author(s):  
U. Canosi ◽  
A. Iglesias ◽  
T. A. Trautner





2018 ◽  
Vol 9 ◽  
Author(s):  
Ester Serrano ◽  
Begoña Carrasco ◽  
Jamie L. Gilmore ◽  
Kunio Takeyasu ◽  
Juan C. Alonso


1985 ◽  
Vol 198 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Juan Carlos Alonso ◽  
Thomas A. Trautner


2001 ◽  
Vol 183 (19) ◽  
pp. 5772-5777 ◽  
Author(s):  
Begoña Carrasco ◽  
Silvia Fernández ◽  
Marie-Agnes Petit ◽  
Juan C. Alonso

ABSTRACT The B. subtilis ΔhelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec+ strain. The ΔhelD allele was introduced into rec-deficient strains representative of the α (recF strain), β (addA addB), γ (recH), ɛ (ΔrecU), and ζ (ΔrecS) epistatic groups. The ΔhelDmutation increased the sensitivity to DNA-damaging agents ofaddAB, ΔrecU, and ΔrecS cells, did not affect the survival ofrecH cells, and decreased the sensitivity ofrecF cells. ΔhelD also partially suppressed the DNA repair phenotype of other mutations classified within the α epistatic group, namely the recL, ΔrecO, and recR mutations. The ΔhelD allele marginally reduced plasmid transformation (three- to sevenfold) of mutations classified within the α, β, and γ epistatic groups. Altogether, these data indicate that the loss of helicase IV might stabilize recombination repair intermediates formed in the absence of recFLOR and renderrecFLOR, addAB, andrecH cells impaired in plasmid transformation.



1987 ◽  
Vol 210 (3) ◽  
pp. 476-484 ◽  
Author(s):  
Juan C. Alonso ◽  
Ravindra H. Tailor


1983 ◽  
Vol 192 (1-2) ◽  
pp. 149-154 ◽  
Author(s):  
Antonio Iglesias ◽  
Piotr Ceglowski ◽  
Thomas A. Trautner


1990 ◽  
Vol 67 (1-2) ◽  
pp. 227-230 ◽  
Author(s):  
Yuko Matsuno ◽  
Hideji Hiraoka ◽  
Takashi Ano ◽  
Makoto Shoda


1981 ◽  
Vol 184 (3) ◽  
pp. 400-404 ◽  
Author(s):  
G. Bensi ◽  
A. Iglesias ◽  
U. Canosi ◽  
T. A. Trautner


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