Cellular inactivation and mitotic recombination induced by ultraviolet radiation in aneuploid and euploid strains of Candida albicans

1984 ◽  
Vol 87 (1-2) ◽  
pp. 35-41 ◽  
Author(s):  
Douglas D. Rhoads ◽  
Alvin Sarachek
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation ◽  
Mitotic Recombination

Effect of Ultraviolet Radiation on Candida albicans Biofilm on Poly(methylmethacrylate) Resin

2020 ◽  
Vol 29 (8) ◽  
pp. 686-692
Author(s):  
Randold Binns ◽  
Wei Li ◽  
Christine D. Wu ◽  
Stephen Campbell ◽  
Kent Knoernschild ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation

scholarly journals Partner Choice in Spontaneous Mitotic Recombination in Wild Type and Homologous Recombination Mutants of Candida albicans

2019 ◽  
Vol 9 (11) ◽  
pp. 3631-3644
Author(s):  
Alberto Bellido ◽  
Toni Ciudad ◽  
Belén Hermosa ◽  
Encarnación Andaluz ◽  
Anja Forche ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Homologous Recombination ◽  
Mitotic Recombination ◽  
Partner Choice ◽  
Wild Type

Differential effects of growth temperatures on inactivation and mutation of Candida albicans by ultraviolet radiation

1969 ◽  
Vol 67 (2) ◽  
pp. 189-198 ◽  
Author(s):  
A. Sarachek ◽  
R. V. Goering ◽  
J. T. Bish
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation ◽  
Differential Effects

scholarly journals Prevention of ultraviolet radiation-induced immunosuppression by sunscreen in Candida albicans-induced delayed-type hypersensitivity

2016 ◽  
Vol 14 (1) ◽  
pp. 202-208 ◽  
Author(s):  
QUAN CHEN ◽  
RUNXIANG LI ◽  
XIAOXIA ZHAO ◽  
BIHUA LIANG ◽  
SHAOYIN MA ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation ◽  
Delayed Type Hypersensitivity ◽  
Radiation Induced

Relative susceptibilities of caffeine-sensitive and caffeine-resistant strains of Candida albicans to inactivation and mutation by ultraviolet radiation

1970 ◽  
Vol 74 (3) ◽  
pp. 244-257 ◽  
Author(s):  
A. Sarachek ◽  
J. T. Bish ◽  
Rosalee Ireland
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation ◽  
Resistant Strains

Enhancement of Candida albicans killing activity of separated human epidermal cells by ultraviolet radiation

1987 ◽  
Vol 116 (4) ◽  
pp. 469-475 ◽  
Author(s):  
M. CSATO ◽  
ANNA SZ. KENDERESSY ◽  
A. DOBOZY
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation ◽  
Epidermal Cells ◽  
Killing Activity ◽  
Human Epidermal Cells

Inactivation of Candida albicans by ultraviolet radiation

1969 ◽  
Vol 64 (4) ◽  
pp. 289-314 ◽  
Author(s):  
David L. Busbee ◽  
Alvin Sarachek
Keyword(s):  
Candida Albicans ◽  
Ultraviolet Radiation

scholarly journals Stress Alters Rates and Types of Loss of Heterozygosity in Candida albicans

mBio ◽  
2011 ◽  
Vol 2 (4) ◽  
Author(s):  
A. Forche ◽  
D. Abbey ◽  
T. Pisithkul ◽  
M. A. Weinzierl ◽  
T. Ringstrom ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Genetic Diversity ◽  
Candida Albicans ◽  
Loss Of Heterozygosity ◽  
Mitotic Recombination ◽  
Stress Conditions ◽  
Dependent Manner ◽  
Content Type ◽  
Different Types ◽  
Stress Dependent

ABSTRACTGenetic diversity is often generated during adaptation to stress, and in eukaryotes some of this diversity is thought to arise via recombination and reassortment of alleles during meiosis.Candida albicans, the most prevalent pathogen of humans, has no known meiotic cycle, and yet it is a heterozygous diploid that undergoes mitotic recombination during somatic growth. It has been shown that clinical isolates as well as strains passaged once through a mammalian host undergo increased levels of recombination. Here, we tested the hypothesis that stress conditions increase rates of mitotic recombination inC. albicans, which is measured as loss of heterozygosity (LOH) at specific loci. We show that LOH rates are elevated duringin vitroexposure to oxidative stress, heat stress, and antifungal drugs. In addition, an increase in stress severity correlated well with increased LOH rates. LOH events can arise through local recombination, through homozygosis of longer tracts of chromosome arms, or by whole-chromosome homozygosis. Chromosome arm homozygosis was most prevalent in cultures grown under conventional lab conditions. Importantly, exposure to different stress conditions affected the levels of different types of LOH events, with oxidative stress causing increased recombination, while fluconazole and high temperature caused increases in events involving whole chromosomes. Thus,C. albicansgenerates increased amounts and different types of genetic diversity in response to a range of stress conditions, a process that we term “stress-induced LOH” that arises either by elevating rates of recombination and/or by increasing rates of chromosome missegregation.IMPORTANCEStress-induced mutagenesis fuels the evolution of bacterial pathogens and is mainly driven by genetic changes via mitotic recombination. Little is known about this process in other organisms.Candida albicans, an opportunistic fungal pathogen, causes infections that require adaptation to different host environmental niches. We measured the rates of LOH and the types of LOH events that appeared in the absence and in the presence of physiologically relevant stresses and found that stress causes a significant increase in the rates of LOH and that this increase is proportional to the degree of stress. Furthermore, the types of LOH events that arose differed in a stress-dependent manner, indicating that eukaryotic cells generate increased genetic diversity in response to a range of stress conditions. We propose that this “stress-induced LOH” facilitates the rapid adaptation ofC. albicans, which does not undergo meiosis, to changing environments within the host.

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